Muscle-powered Cantilever for Microtweezers with an Artificial Micro Skeleton and Rat Primary Myotubes

A technique to attach muscle cells to artificial micro mechanical systems was studied using dog body hair and rat primary skeletal muscle. Muscle-powered cantilever for microtweezers were fabricated which consisted of a single strand of hair for the skeleton and differentiated myotubes for the actuator. The three-dimensional mechanical part of the microtweezers was fabricated using a focused ion beam-induced ion milling technique. The micro hair skeleton was used as a scaffold for the muscular cells and the mechanical structure. Electrical stimulation induced related contraction of the myotubes and displacement of the muscle-powered cantilever of the microtweezers, although the displacement was not yet enough for useful microtools.

[1]  J. Xi,et al.  Self-assembled microdevices driven by muscle , 2005, Nature materials.

[2]  N Imbert,et al.  Appearance and evolution of calcium currents and contraction during the early post-fusional stages of rat skeletal muscle cells developing in primary culture. , 1993, Development.

[3]  S Sugiura,et al.  A novel method to study contraction characteristics of a single cardiac myocyte using carbon fibers. , 2001, American journal of physiology. Heart and circulatory physiology.

[4]  Joan E Sanders,et al.  Tissue engineering of skeletal muscle using polymer fiber arrays. , 2003, Tissue engineering.

[5]  G. Whitesides,et al.  Muscular Thin Films for Building Actuators and Powering Devices , 2007, Science.

[6]  Byungkyu Kim,et al.  Establishment of a fabrication method for a long-term actuated hybrid cell robot. , 2007, Lab on a chip.

[7]  W. Zimmermann,et al.  Tissue Engineering of a Differentiated Cardiac Muscle Construct , 2002, Circulation research.

[8]  Keith Baar,et al.  Rapid formation of functional muscle in vitro using fibrin gels. , 2005, Journal of applied physiology.

[9]  Takehiko Kitamori,et al.  Demonstration of a PDMS-based bio-microactuator using cultured cardiomyocytes to drive polymer micropillars. , 2006, Lab on a chip.

[10]  Bharat Bhushan,et al.  Nanotribological and nanomechanical characterization of human hair using a nanoscratch technique. , 2006, Ultramicroscopy.

[11]  Takehiko Kitamori,et al.  An actuated pump on-chip powered by cultured cardiomyocytes. , 2006, Lab on a chip.

[12]  K. Wilson,et al.  A Defined System to Allow Skeletal Muscle Differentiation and Subsequent Integration with Silicon Microstructures , 1999 .

[13]  Robert G. Dennis,et al.  Excitability and isometric contractile properties of mammalian skeletal muscle constructs engineered in vitro , 2000, In Vitro Cellular & Developmental Biology - Animal.

[14]  R G Dennis,et al.  Excitability and contractility of skeletal muscle engineered from primary cultures and cell lines. , 2001, American journal of physiology. Cell physiology.

[15]  Kunihiko Mabuchi,et al.  Development of a regeneration-type neural interface : A microtube guide for axon growth of neuronal cells fabricated using focused-ion-beam chemical vapor deposition , 2006 .

[16]  R. Bonser,et al.  The Young's modulus of feather keratin , 1995, The Journal of experimental biology.

[17]  G. Julius Vancso,et al.  Hydrophobic recovery of UV/ozone treated poly(dimethylsiloxane): adhesion studies by contact mechanics and mechanism of surface modification , 2005 .