Fabrication of a Highly Sensitive Electrochemical Immunosensor for Human Epididymis Protein 4 (HE4) Detection

Diagnostics of ovarian cancer can be achieved by detecting the level of human epididymis protein 4 (HE4). The present study proposed the preparation of a biosensor based on a nitrogen-doped graphene/gold nanoparticle (AuNP)/fluorine-doped tin oxide (FTO) glass electrode modified with fructosyl amino-acid oxidase (FAO), i.e., the AuNPs/N-doped GNs/FTO. The proposed electrode was found to be highly sensitive and selective towards the biosensing of HE4, with a the limit of detection (LOD) calculated at 1.21 μg/mL and a linear detection range of 10 to 65 μg/mL.