In vivo fluorescent imaging of the mouse retina using adaptive optics.

In vivo imaging of the mouse retina using visible and near infrared wavelengths does not achieve diffraction-limited resolution due to wavefront aberrations induced by the eye. Considering the pupil size and axial dimension of the eye, it is expected that unaberrated imaging of the retina would have a transverse resolution of 2 microm. Higher-order aberrations in retinal imaging of human can be compensated for by using adaptive optics. We demonstrate an adaptive optics system for in vivo imaging of fluorescent structures in the retina of a mouse, using a microelectromechanical system membrane mirror and a Shack-Hartmann wavefront sensor that detects fluorescent wavefront.

[1]  Geunyoung Yoon,et al.  Use of a microelectromechanical mirror for adaptive optics in the human eye. , 2002, Optics letters.

[2]  D R Williams,et al.  Supernormal vision and high-resolution retinal imaging through adaptive optics. , 1997, Journal of the Optical Society of America. A, Optics, image science, and vision.

[3]  Michel Paques,et al.  High resolution fundus imaging by confocal scanning laser ophthalmoscopy in the mouse , 2006, Vision Research.

[4]  Joseph W. Haus,et al.  OSA Trends in Optics and Photonics Series , 2003 .

[5]  David Williams,et al.  The arrangement of the three cone classes in the living human eye , 1999, Nature.

[6]  David H Sliney,et al.  Maximum permissible exposures for ocular safety (ANSI 2000), with emphasis on ophthalmic devices. , 2007, Journal of the Optical Society of America. A, Optics, image science, and vision.

[7]  Yaopeng Zhou,et al.  Stroke amplifier for deformable mirrors. , 2004, Applied optics.

[8]  Siddharth Poonja,et al.  MEMS-based adaptive optics scanning laser ophthalmoscopy , 2006 .

[9]  Susana Marcos,et al.  Optical aberrations in the mouse eye , 2006, Vision Research.

[10]  Kazuhisa Sugiyama,et al.  In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope. , 2006, Investigative ophthalmology & visual science.

[11]  Bernard P. Gee,et al.  In vivo fluorescence imaging of primate retinal ganglion cells and retinal pigment epithelial cells. , 2006, Optics express.

[12]  R. D. Ferguson,et al.  Adaptive optics scanning laser ophthalmoscope for stabilized retinal imaging. , 2006, Optics express.

[13]  R. Rosenfeld.  Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[14]  T. Hebert,et al.  Adaptive optics scanning laser ophthalmoscopy. , 2002, Optics express.