Isolation and structure of somatostatin from porcine hypothalami.

The isolation and structure of somatostatin (GH-RIH) from pig hypothalami are described. This hormone was purified by preparative gel filtration, solvent extraction, countercurrent distribution in two solvent systems, ion-exchange and partition chromatography, and analytical gel filtration. The somatostatin activity was followed by in vitro bioassays and a radioimmunoassay. The isolated product was homogeneous chromatographically and had biological and immunological properties similar to synthetic somatostatin corresponding to the ovine hormone. The primary structure of porcine somatostatin was shown to be H-Ala-Gly-cyclo-(Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys)-OH. Other immunologically and biologically active form(s) of somatostatin were also detected.

[1]  A. Schally,et al.  Somatostatin: abundance of immunoreactive hormone in rat stomach and pancreas. , 1975, Science.

[2]  A. Schally,et al.  Radioimmunoassay for GH-Release Inhibiting Hormone 1 , 1975, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[3]  R. Unger,et al.  Somatostatin-induced changes in insulin and glucagon secretion in normal and diabetic dogs. , 1974, The Journal of clinical investigation.

[4]  R. Guillemin,et al.  Isolation of somatostatin (a somatotropin release inhibiting factor) of ovine hypothalamic origin. , 1974, Canadian journal of biochemistry.

[5]  W. Ganong,et al.  Effect of synthetic somatotropin release inhibiting factor on the increase in plasma growth hormone elicited by L-dopa in the dog. , 1974, Endocrinology.

[6]  K. Sestanj,et al.  Synthesis of Somatostatin. Preliminary communication , 1974 .

[7]  C. J. Goodner,et al.  Somatostatin: Hypothalamic Inhibitor of the Endocrine Pancreas , 1974, Science.

[8]  S. Yen,et al.  Effect of somatostatin in patients with acromegaly: suppression of growth hormone, prolactin, insulin and glucose levels. , 1974, The New England journal of medicine.

[9]  R. Sauer,et al.  The amino acid sequence of porcine parathyroid hormone. , 1974, Biochemistry.

[10]  A. Schally,et al.  Solid phase synthesis of growth hormone-release inhibiting factor. , 1973, Biochemical and biophysical research communications.

[11]  R. Guillemin,et al.  Inhibition of growth hormone release in humans by somatostatin. , 1973, The Journal of clinical endocrinology and metabolism.

[12]  D. Yamashiro,et al.  Synthesis of a peptide with full somatostatin activity. , 1973, Biochemical and biophysical research communications.

[13]  A. Schally,et al.  Influence of previous cooling of rat pituitaries on the release of GH, LH and prolactin in vitro. , 1973, Neuroendocrinology.

[14]  N. Ling,et al.  Primary structure of somatostatin, a hypothalamic peptide that inhibits the secretion of pituitary growth hormone. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[15]  N. Ling,et al.  Hypothalamic Polypeptide That Inhibits the Secretion of Immunoreactive Pituitary Growth Hormone , 1973, Science.

[16]  R. Spalding,et al.  Uranium in runoff. , 1972, Science.

[17]  R. Guillemin,et al.  Culture of Enzymatically Dispersed Anterior Pituitary Cells: Functional Validation of a Method , 1972 .

[18]  A. Schally,et al.  Isolation of the luteinizing hormone and follicle-stimulating hormone-releasing hormone from porcine hypothalami. , 1971, The Journal of biological chemistry.

[19]  T. J. Bronzert,et al.  Analysis of amino acid phenylthiohydantoins by gas chromatography. , 1969, The Journal of biological chemistry.

[20]  A. Schally,et al.  Isolation and properties of porcine thyrotropin-releasing hormone. , 1969, The Journal of biological chemistry.

[21]  D. Schalch,et al.  Plasma growth hormone concentration in the rat determined by radioimmunoassay: influence of sex, pregnancy, lactation, anesthesia, hypophysectomy and extrasellar pituitary transplants. , 1966, Endocrinology.

[22]  J. Porath,et al.  Gel filtration of posterior pituitary hormones. , 1962, Endocrinology.

[23]  P. Edman.  PHENYLTHIOHYDANTOINS IN PROTEIN ANALYSIS , 1960, Annals of the New York Academy of Sciences.