Dietary Iron Deficiency Decreases Serum Osteocalcin Concentration and Bone Mineral Density in Rats

We investigated the effects of dietary iron deficiency on bone metabolism by measuring markers of bone turnover in rats. Twelve 3-week-old male Wistar-strain rats were fed a control diet or an iron-deficient diet for 4 weeks. Dietary iron deficiency decreased hemoglobin concentration and increased heart weight. Serum osteocalcin concentration, bone mineral content, bone mineral density, and mechanical strength of the femur were significantly lower in the iron-deficient group than in the control group. These results suggested that dietary iron deficiency affected bone, which might have been due to a decrease in bone formation in rats.

[1]  L. Houtkooper,et al.  Dietary iron positively influences bone mineral density in postmenopausal women on hormone replacement therapy. , 2005, The Journal of nutrition.

[2]  M. Haub,et al.  Iron deficiency negatively affects vertebrae and femurs of rats independently of energy intake and body weight. , 2004, The Journal of nutrition.

[3]  L. Houtkooper,et al.  Dietary iron is associated with bone mineral density in healthy postmenopausal women. , 2003, The Journal of nutrition.

[4]  D. Jennings,et al.  Bone morphology, strength and density are compromised in iron-deficient rats and exacerbated by calcium restriction. , 2002, The Journal of nutrition.

[5]  M. Uehara,et al.  Dose-responsive alteration in hepatic lipid peroxidation and retinol metabolism with increasing dietary beta-carotene in iron deficient rats. , 2002, International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition.

[6]  Y. Kanke,et al.  Time Course of Change in Hepatic Lipid Peroxide Level in Iron-Deficient Rats. , 1998 .

[7]  M. Uehara,et al.  Induction of increased phosphatidylcholine hydroperoxide by an iron-deficient diet in rats , 1997 .

[8]  V. Matkovic,et al.  Femurs from rats fed diets deficient in copper or iron have decreased mechanical strength and altered mineral composition , 1997 .

[9]  C. Christiansen,et al.  Immunoassay for quantifying type I collagen degradation products in urine evaluated. , 1994, Clinical chemistry.

[10]  M. Serio,et al.  Comparison of immuno- and HPLC-assays for the measurement of urinary collagen cross-links , 1994, Journal of endocrinological investigation.

[11]  P. G. Reeves,et al.  AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. , 1993, The Journal of nutrition.

[12]  P. Price,et al.  Bone Gla protein messenger ribonucleic acid is regulated by both 1,25-dihydroxyvitamin D3 and 3',5'-cyclic adenosine monophosphate in rat osteosarcoma cells. , 1989, Molecular endocrinology.

[13]  G. Rodan,et al.  Characterization of the rat osteocalcin gene: stimulation of promoter activity by 1,25-dihydroxyvitamin D3. , 1988, Biochemistry.

[14]  P. Delmas,et al.  SERUM BONE GLA-PROTEIN: A SPECIFIC MARKER FOR BONE FORMATION IN POSTMENOPAUSAL OSTEOPOROSIS , 1984, The Lancet.

[15]  K. Kivirikko,et al.  Mechanism of the Prolyl Hydroxylase Reaction , 1982 .

[16]  K. Kivirikko,et al.  Mechanism of the prolyl hydroxylase reaction. 1. Role of co-substrates. , 1977, European journal of biochemistry.

[17]  H. DeLuca,et al.  Metabolism of vitamin D: current status. , 1976, The American journal of clinical nutrition.

[18]  J. Winberg,et al.  Does breast milk protect against septicaemia in the newborn? , 1971, Lancet.