Yeast Whi2 and Psr1‐phosphatase form a complex and regulate STRE‐mediated gene expression

Background:  In response to various stressful situations, including diauxic conditions, the Msn2 and Msn4 transcription factors induce STRE‐mediated gene expression of many stress responsive genes in Saccharomyces cerevisiae. This is called the general stress response. The whi2 cells in the stationary phase are smaller than wild‐type cells.

[1]  F. Estruch.  Stress-controlled transcription factors, stress-induced genes and stress tolerance in budding yeast. , 2000, FEMS microbiology reviews.

[2]  A. Fujita,et al.  The MCS1/SSD1/SRK1/SSL1 gene is involved in stable maintenance of the chromosome in yeast. , 1994, Gene.

[3]  Jonathan A. Cooper,et al.  Mammalian Ras interacts directly with the serine/threonine kinase raf , 1993, Cell.

[4]  M. Sekiguchi.  Genes to cells: edited by Jun-ichi Tomizawa, Blackwell Science Ltd. Institutional: £218.00 (Europe), £242.00 (Rest of World), US$382.00 (USA and Canada). Individual: £65.00 (Europe), £72.00 (Rest of World), US$114.00 (USA and Canada) ISSN 1356 9597 , 1997 .

[5]  Nihon Hassei Seibutsu Gakkai,et al.  Genes to cells , 1996 .

[6]  A. Marchler-Bauer,et al.  The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). , 1996, The EMBO journal.

[7]  Michael R. Green,et al.  Dissecting the Regulatory Circuitry of a Eukaryotic Genome , 1998, Cell.

[8]  M. Jacquet,et al.  Hyperphosphorylation of Msn2p and Msn4p in response to heat shock and the diauxic shift is inhibited by cAMP in Saccharomyces cerevisiae. , 2000, Microbiology.

[9]  M. Ward,et al.  Yeast PKA represses Msn2p/Msn4p‐dependent gene expression to regulate growth, stress response and glycogen accumulation , 1998, The EMBO journal.

[10]  M. Carlson,et al.  Two homologous zinc finger genes identified by multicopy suppression in a SNF1 protein kinase mutant of Saccharomyces cerevisiae , 1993, Molecular and cellular biology.

[11]  Michael N. Hall,et al.  The TOR signalling pathway controls nuclear localization of nutrient-regulated transcription factors , 1999, Nature.

[12]  R. D. Gietz,et al.  New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. , 1988, Gene.

[13]  B Hamilton,et al.  Nuclear localization of the C2H2 zinc finger protein Msn2p is regulated by stress and protein kinase A activity. , 1998, Genes & development.

[14]  K. Köhrer,et al.  Preparation of high molecular weight RNA. , 1991, Methods in enzymology.

[15]  Hilla Peretz,et al.  Ju n 20 03 Schrödinger ’ s Cat : The rules of engagement , 2003 .

[16]  T. Sasaki,et al.  Extragenic suppressors that rescue defects in the heat stress response of the budding yeast mutant tom1 , 2000, Molecular and General Genetics MGG.

[17]  G. Fink,et al.  Methods in yeast genetics , 1979 .

[18]  Y. Kikuchi,et al.  Yeast Krr1p Physically and Functionally Interacts with a Novel Essential Kri1p, and Both Proteins Are Required for 40S Ribosome Biogenesis in the Nucleolus , 2000, Molecular and Cellular Biology.

[19]  B. Carter,et al.  Genes which control cell proliferation in the yeast Saccharomyces cerevisiae , 1980, Nature.

[20]  R. Young,et al.  Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase. , 2001, Genes & development.

[21]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[22]  B. Cairns,et al.  Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase. , 1992, Genes & development.

[23]  M. Tyers,et al.  Deregulation of CLN1 and CLN2 in the Saccharomyces cerevisiae whi2 Mutant , 1997, Yeast.

[24]  H. Yashiroda,et al.  Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae , 1996, Molecular and Cellular Biology.

[25]  G. Adam,et al.  A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. , 1993, The EMBO journal.

[26]  L. Prakash,et al.  Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers , 1990, Yeast.

[27]  H. Pelham,et al.  Psr1p/Psr2p, Two Plasma Membrane Phosphatases with an Essential DXDX(T/V) Motif Required for Sodium Stress Response in Yeast* , 2000, The Journal of Biological Chemistry.

[28]  A. Schmitt,et al.  Msn2p, a zinc finger DNA-binding protein, is the transcriptional activator of the multistress response in Saccharomyces cerevisiae. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[29]  S. Fields,et al.  The two-hybrid system: an assay for protein-protein interactions. , 1994, Trends in genetics : TIG.

[30]  I. Yamashita,et al.  A transcriptional autoregulatory loop for KIN28–CCL1 and SRB10–SRB11, each encoding RNA polymerase II CTD kinase–cyclin pair, stimulates the meiotic development of S. cerevisiae , 2000, Yeast.

[31]  P. Radcliffe,et al.  The yeast PRS3 gene is required for cell integrity, cell cycle arrest upon nutrient deprivation, ion homeostasis and the proper organization of the actin cytoskeleton , 1999, Yeast.