Reconstruction of an in vitro cornea and its use for drug permeation studies from different formulations containing pilocarpine hydrochloride.

The aim of the present contribution was to develop a functional three-dimensional tissue construct to study ocular permeation of pilocarpine hydrochloride from different formulations. The in vitro model was compared to excised bovine cornea. Modified Franz cells were used to study the transcorneal permeability. Analysis was performed by reversed-phase high-performance liquid chromatography. Comparisons of the permeation rates through excised bovine cornea and the in vitro model show the same rank order for the different formulations. The permeation coefficient, K(P), obtained with the cornea construct, is about 2-4-fold higher than that from excised bovine cornea. It is possible to reconstruct bovine cornea as an organotypic culture and also to use this construct as a substitute for excised bovine cornea in drug permeation studies in vitro.

[1]  A. I. Schneider,et al.  The use of an in vitro cornea for predicting ocular toxicity , 1997 .

[2]  S. Keipert,et al.  Influence of alpha-cyclodextrin and hydroxyalkylated beta-cyclodextrin derivatives on the in vitro corneal uptake and permeation of aqueous pilocarpine-HCl solutions. , 1997, Journal of pharmaceutical sciences.

[3]  D. Tang-Liu,et al.  A corneal perfusion device for estimating ocular bioavailability in vitro. , 1990, Journal of Pharmacy and Science.

[4]  J. Zieske,et al.  Basement membrane assembly and differentiation of cultured corneal cells: importance of culture environment and endothelial cell interaction. , 1994, Experimental cell research.

[5]  S. Ledbetter,et al.  Bovine corneal endothelium in vitro. Elaboration and organization and of a basement membrane. , 1982, Experimental cell research.

[6]  B. Yue,et al.  Mass culture of human corneal endothelial cells. , 1979, Archives of ophthalmology.

[7]  C. Kahn,et al.  Human corneal epithelial primary cultures and cell lines with extended life span: in vitro model for ocular studies. , 1993, Investigative ophthalmology & visual science.

[8]  J. Friend,et al.  Comparison of limbal and peripheral human corneal epithelium in tissue culture. , 1988, Investigative ophthalmology & visual science.

[9]  P. Friedl,et al.  Isolation and long-term cultivation of human corneal endothelial cells. , 1988, Investigative ophthalmology & visual science.

[10]  H. Sugihara,et al.  Reconstruction of cornea in three-dimensional collagen gel matrix culture. , 1993, Investigative ophthalmology & visual science.

[11]  M. Conconi,et al.  Bovine corneal stroma and epithelium reconstructed in vitro: Characterisation and response to surfactants , 1998, Eye.

[12]  A. Urtti,et al.  Permeability of pilocarpic acid diesters across albino rabbit cornea in vitro , 1991 .

[13]  E Bell,et al.  Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. , 1981, Science.

[14]  N. Georgiade,et al.  A tissue culture technique for growing corneal epithelial, stromal, and endothelial tissues separately. , 1958, American journal of ophthalmology.

[15]  C. Müller-Goymann,et al.  Comparative investigations to evaluate the use of organotypic cultures of transformed and native dermal and epidermal cells for permeation studies. , 1998, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.