INHIBITION OF RESPIRATION AND LOSS OF MEMBRANE INTEGRITY BY SINGLET OXYGEN GENERATED BY A PHOTOSENSITIZED REACTION IN NEUROSPORA CRASSA CONIDIA

Abstract— It was found that killing of Neurospora crassa conidia by singlet molecular oxygen generated by a photosensitized reaction in the presence of toluidine blue O, accompanied inhibition of respiration and uptake of the photosensitizing dye by conidia. Both the inactivation of respiration and the uptake of toluidine blue O were stimulated by deuterium oxide and suppressed by azide. Mitochondria of irradiated conidia were swollen and showed matrix of low density under an electron microscope.

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