The present study describes the application of sodium dodecyl sulfate micellar electrokinetic chromatography (SDS MEKC) for the fast analysis of TNT biotransformation products in the aqueous phase of anaerobic sludge cultures. SDS MEKC was performed using a Hewlett-Packard HP 3D CE capillary electrophoresis system with photodiode array detection. In a single sample injection the SDS MEKC method detected 2,4,6-trinitrotoluene (TNT), 2-hydroxylamino-4,6-dinitrotoluene (2-HADNT), 4-hydroxylamino-2,6-dinitrotoluene (4-HADNT), 2-amino-4,6-dinitrotoluene (2-ADNT), 4-amino-2,6-dinitrotoluene (4-ADNT), 2,4-diaminonitrotoluene (2,4-DANT), 2,6-diaminonitrotoluene (2,6-DANT), and 2,4,6-triaminotoluene (TAT). Analyte peaks were verified using visible/ultraviolet photodiode array spectra from commercial standards and periodic analyses using electrospray ionization liquid chromatography mass spectrometry (ES-LC-MS). A time course study constructed from the SDS MEKC data provided supporting evidence for the stepwise red...