Ultrasound-Assisted Microencapsulation of Soybean Oil and Vitamin D Using Bare Glycogen Nanoparticles

Ultrasonically synthesized core-shell microcapsules can be made of synthetic polymers or natural biopolymers, such as proteins and polysaccharides, and have found applications in food, drug delivery and cosmetics. This study reports on the ultrasonic synthesis of microcapsules using unmodified (natural) and biodegradable glycogen nanoparticles derived from various sources, such as rabbit and bovine liver, oyster and sweet corn, for the encapsulation of soybean oil and vitamin D. Depending on their source, glycogen nanoparticles exhibited differences in size and ‘bound’ proteins. We optimized various synthetic parameters, such as ultrasonic power, time and concentration of glycogens and the oil phase to obtain stable core-shell microcapsules. Particularly, under ultrasound-induced emulsification conditions (sonication time 45 s and sonication power 160 W), native glycogens formed microcapsules with diameter between 0.3 μm and 8 μm. It was found that the size of glycogen as well as the protein component play an important role in stabilizing the Pickering emulsion and the microcapsules shell. This study highlights that native glycogen nanoparticles without any further tedious chemical modification steps can be successfully used for the encapsulation of nutrients.

[1]  S. Shaarani,et al.  Nutritional values and microencapsulation techniques of fish oil from different sources: A mini review , 2021 .

[2]  Yangchao Luo,et al.  Properties and applications of natural dendritic nanostructures: Phytoglycogen and its derivatives , 2020 .

[3]  M. Ashokkumar,et al.  Ultrasonic emulsification: An overview on the preparation of different emulsifiers-stabilized emulsions , 2020 .

[4]  Li Li,et al.  Recent advances in polysaccharides stabilized emulsions for encapsulation and delivery of bioactive food ingredients: A review. , 2020, Carbohydrate polymers.

[5]  F. Caruso,et al.  The Protein Component of Oyster Glycogen Nanoparticles: An Anchor Point for Functionalization. , 2020, ACS applied materials & interfaces.

[6]  T. Mehmood,et al.  Tween 80 and Soya Lecithin Based Food Grade Nanoemulsions for the Effective Delivery of Vitamin D. , 2020, Langmuir : the ACS journal of surfaces and colloids.

[7]  Vaibhav Kumar Maurya,et al.  Vitamin D microencapsulation and fortification: Trends and technologies , 2020, The Journal of Steroid Biochemistry and Molecular Biology.

[8]  D. Mcclements,et al.  Encapsulation of Vitamin D3 in Pickering Emulsion Stabilized by Nanofibrillated Mangosteen Cellulose: Effect of Environmental Stresses. , 2019, Journal of food science.

[9]  F. Caruso,et al.  Glycogen as a Building Block for Advanced Biological Materials , 2019, Advanced materials.

[10]  T. Alkie,et al.  Enhancing innate antiviral immune responses in rainbow trout by double stranded RNA delivered with cationic phytoglycogen nanoparticles , 2019, Scientific Reports.

[11]  M. Suphantharika,et al.  Design and synthesis of modified and resistant starch-based oil-in-water emulsions , 2019, Food Hydrocolloids.

[12]  F. Caruso,et al.  Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids. , 2018, Biomaterials.

[13]  B. Schulz,et al.  Proteomic Investigation of the Binding Agent between Liver Glycogen β Particles , 2018, ACS omega.

[14]  Crispin R Dass,et al.  Microparticles, microcapsules and microspheres: A review of recent developments and prospects for oral delivery of insulin. , 2018, International journal of pharmaceutics.

[15]  M. Ashokkumar,et al.  Ultrasonic encapsulation - A review. , 2017, Ultrasonics sonochemistry.

[16]  Y. Yao,et al.  Alpha-D-glucan nanoparticulate adjuvant induces a transient inflammatory response at the injection site and targets antigen to migratory dendritic cells , 2017, npj Vaccines.

[17]  Qian Liu,et al.  Improvement of the emulsifying and oxidative stability of myofibrillar protein prepared oil-in-water emulsions by addition of zein hydrolysates , 2017 .

[18]  E. Chan,et al.  Effects of environmental factors on the physical stability of pickering-emulsions stabilized by chitosan particles , 2016 .

[19]  G. Garcia-Garcia,et al.  Pickering Particles Prepared from Food Waste , 2016, Materials.

[20]  Youngsoo Lee,et al.  Surface modification of zein colloidal particles with sodium caseinate to stabilize oil-in-water pickering emulsion , 2016 .

[21]  G. Ehlers,et al.  Structure and Hydration of Highly-Branched, Monodisperse Phytoglycogen Nanoparticles. , 2016, Biomacromolecules.

[22]  Marco Caggioni,et al.  Encapsulation and Enhanced Retention of Fragrance in Polymer Microcapsules. , 2016, ACS applied materials & interfaces.

[23]  K. Tam,et al.  Stimuli-responsive Pickering emulsions: recent advances and potential applications. , 2015, Soft matter.

[24]  Y. Yao,et al.  Dendrimer-like alpha-d-glucan nanoparticles activate dendritic cells and are effective vaccine adjuvants. , 2015, Journal of controlled release : official journal of the Controlled Release Society.

[25]  Xiaoyan Song,et al.  Preparation and characterizations of Pickering emulsions stabilized by hydrophobic starch particles , 2015 .

[26]  Kun Xu,et al.  Triglyceride-water emulsions stabilised by starch-based nanoparticles , 2014 .

[27]  안톤 코레네브스키,et al.  Monodisperse glycogen and phytoglycogen nanoparticles and use thereof as additives in cosmetics, pharmaceuticals, and food products , 2014 .

[28]  Jingnan Wang,et al.  An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives , 2014, International journal of nanomedicine.

[29]  Yunxing Li,et al.  Pickering emulsions stabilized by native starch granules , 2013 .

[30]  David Julian McClements,et al.  Encapsulation of functional lipophilic components in surfactant-based colloidal delivery systems: vitamin E, vitamin D, and lemon oil. , 2012, Food chemistry.

[31]  K. Velikov,et al.  Oil-in-water Pickering emulsions stabilized by colloidal particles from the water-insoluble protein zein , 2012 .

[32]  F. von Delft,et al.  Conformational plasticity of glycogenin and its maltosaccharide substrate during glycogen biogenesis , 2011, Proceedings of the National Academy of Sciences.

[33]  B. Bhandari,et al.  Influence of emulsion droplet size on antimicrobial properties , 2011 .

[34]  Y. Yao,et al.  In vitro digestibility and emulsification properties of phytoglycogen octenyl succinate. , 2010, Journal of agricultural and food chemistry.

[35]  J. Anzai,et al.  Preparation of insulin-containing microcapsules by a layer-by-layer deposition of concanavalin A and glycogen. , 2009, Journal of nanoscience and nanotechnology.

[36]  Muthupandian Ashokkumar,et al.  Ultrasonic synthesis of stable, functional lysozyme microbubbles. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[37]  A. Cucheval,et al.  A study on the emulsification of oil by power ultrasound. , 2008, Ultrasonics sonochemistry.

[38]  S. Sacanna,et al.  Thermodynamically stable pickering emulsions. , 2007, Physical review letters.

[39]  Bernard P. Binks,et al.  Emulsions stabilised solely by colloidal particles , 2003 .

[40]  T. Jesionowski.  Effect of surfactants on the size and morphology of the silica particles prepared by an emulsion technique , 2002 .

[41]  P. Roach,et al.  Crystal structure of the autocatalytic initiator of glycogen biosynthesis, glycogenin. , 2002, Journal of molecular biology.

[42]  J. A. Cifonelli,et al.  The Reaction between Concanavalin-A and Glycogen1 , 1956 .