Phylogenetic Analysis of Phanerochaete chrysosporium ITB Isolate Using Internal Transcribed Spacer (ITS) Sequence

The aim of this research is to conduct phylogenetic analyzes of Phanerochaete chrysosporium ITB isolate using DNA sequences of the internal transcribed spacer (ITS) region. The rDNA ITS sequence was developed by using universal ITS5/ITS4 primer pairs. The results showed that the DNA sequence of the ITS region of 700 bp length of Phanerochaete chrysosporium ITB has the highest similarity of 99.6% with PhanerochaetechrysosporiumBKM-F-1767, RP78, PV1, KCTC 6728, SF-4, ATCC MYA- 476, FCL208, FCL236, and Gold-9-419-4. Phanerochaete chrysosporium ITB isolate suspected as new strain of Phanerochaete chrysosporium. Keyword: Phanerochaete chrysosporium, ITS, phylogenetic.

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