Isolation of Primary and Immortalized CD34− Hematopoietic and Mesenchymal Stem Cells from Various Sources

Based on historical radiation experiments in rodents, the hematopoietic stem cell was defined by its biological properties and later by the expression of certain surface antigens (e.g., CD34), as well as the absence of lineage‐specific markers (e.g., DR). Quite recently it was shown that hematopoietic reconstitution can also be achieved by CD34− stem cells, which can be isolated from the bone marrow, peripheral blood and cord blood cells. CD34− stem cells are considered to be predominately part of the quiescent stem cell pool of hematopoietic and mesenchymal stem cells. Due to novel techniques, CD34− stem cells can be expanded on the level of a true stem cell but also directed towards their differentiation into specified tissues or organ systems. This requires the establishment of primary fibroblast‐like CD34− stem cells in vitro and their possible reversible and transient immortalization with optimized vector systems.

[1]  K. Zsebo,et al.  Recombinant human stem cell factor synergises with GM-CSF, G-CSF, IL-3 and epo to stimulate human progenitor cells of the myeloid and erythroid lineages. , 1991, Experimental hematology.

[2]  A. Miller,et al.  Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection , 1990, Molecular and cellular biology.

[3]  I. Weissman,et al.  Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[4]  L. Terstappen,et al.  Lymphoid and myeloid differentiation of single human CD34+, HLA-DR+, CD38- hematopoietic stem cells. , 1994, Blood.

[5]  I. Weissman,et al.  Clonal analysis of hematopoietic stem-cell differentiation in vivo. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[6]  I. Bernstein,et al.  Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin- cells, and the generation of colony-forming cell progeny from CD34+lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor. , 1991, Blood.

[7]  L. Terstappen,et al.  Formation of haematopoietic microenvironment and haematopoietic stem cells from single human bone marrow stem cells , 1992, Nature.

[8]  H. Deeg,et al.  Stem cell factor-induced expression of p27kip-1 during hematopoietic differentiation. , 1995, Experimental and molecular pathology.

[9]  C. March,et al.  Identification of a ligand for the c-kit proto-oncogene , 1990, Cell.

[10]  P. Lansdorp,et al.  Characterization and partial purification of human marrow cells capable of initiating long-term hematopoiesis in vitro. , 1989, Blood.

[11]  L. To,et al.  Circulating autologous stem cells collected in very early remission from acute non‐lymphoblastic leukaemia produce prompt but incomplete haemopoietic reconstitution after high dose melphalan or supralethal chemoradiotherapy , 1985, British journal of haematology.

[12]  S. Nishikawa,et al.  Enrichment and characterization of murine hematopoietic stem cells that express c-kit molecule. , 1991, Blood.

[13]  H. Nakauchi,et al.  Comparison of Hematopoietic Activities of Human Bone Marrow and Umbilical Cord Blood CD34 Positive and Negative Cells , 1999, Stem cells.

[14]  I. Bernstein,et al.  A c-kit ligand, recombinant human stem cell factor, mediates reversible expansion of multiple CD34+ colony-forming cell types in blood and marrow of baboons. , 1992, Blood.

[15]  P. Heinrich,et al.  Quiescence of CD34-negative haematopoietic stem cells is mediated by downregulation of Cyclin B and no stat activation. , 2000, Cytokine.

[16]  V. Chapman,et al.  The proto-oncogene c-kit encoding a transmembrane tyrosine kinase receptor maps to the mouse W locus , 1988, Nature.

[17]  C. Peschle,et al.  KDR receptor: a key marker defining hematopoietic stem cells. , 1999, Science.

[18]  F. Brodsky,et al.  Class II molecules of the major histocompatibility complex considered as differentiation markers. , 1986, Human immunology.

[19]  M. Ogawa,et al.  Reversible expression of CD34 by murine hematopoietic stem cells. , 1999, Blood.

[20]  G. Striker,et al.  Donor origin of the in vitro haematopoietic microenvironment after marrow transplantation in man , 1982, Nature.

[21]  M. Tavassoli,et al.  Hemopoietic stromal microenvironment , 1983, American journal of hematology.

[22]  I. Weissman,et al.  Isolation of a candidate human hematopoietic stem-cell population. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[23]  I. Bernstein,et al.  Precursors of colony-forming cells in humans can be distinguished from colony-forming cells by expression of the CD33 and CD34 antigens and light scatter properties , 1989, The Journal of experimental medicine.

[24]  R Storb,et al.  Bone-marrow transplantation (first of two parts). , 1975, The New England journal of medicine.

[25]  S. Orkin,et al.  Retrovirus-mediated transfer of human adenosine deaminase gene sequences into cells in culture and into murine hematopoietic cells in vivo. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[26]  D. A. Quarles,et al.  Progress and problems , 1953, Electrical Engineering.

[27]  J. Ritz,et al.  Monoclonal Antibody-Purged Autologous Bone Marrow Transplantation Therapy for Multiple Myeloma , 1991 .

[28]  G. Spangrude.  Hematopoietic stem-cell differentiation. , 1991, Current opinion in immunology.

[29]  J. Dick,et al.  A newly discovered class of human hematopoietic cells with SCID-repopulating activity , 1998, Nature Medicine.

[30]  A. Ullrich,et al.  Human proto‐oncogene c‐kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. , 1987, The EMBO journal.

[31]  A. Galy,et al.  Equal distribution of competitive long-term repopulating stem cells in the CD34+ and CD34- fractions of Thy-1lowLin-/lowSca-1+ bone marrow cells. , 1998, Experimental hematology.

[32]  R. Oostendorp,et al.  Constitutive and modulated cytokine expression in two permanent human bone marrow stromal cell lines. , 1996, Experimental hematology.

[33]  R. Samulski,et al.  AAV as a viral vector for human gene therapy , 1995, Molecular biotechnology.

[34]  D. Harris,et al.  Collection, separation and cryopreservation of umbilical cord blood for use in transplantation. , 1994, Bone marrow transplantation.

[35]  H. Broxmeyer,et al.  Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[36]  J. Adamson,et al.  Effects of recombinant human stem cell factor (SCF) on the growth of human progenitor cells in vitro , 1991, Journal of cellular physiology.

[37]  M. Greaves,et al.  Expression of the CD34 gene in vascular endothelial cells. , 1990, Blood.

[38]  E. Cronkite,et al.  Post-Radiation Parabiosis and Survival in Rats.∗ , 1951, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[39]  A. Gown,et al.  Evidence for a stem cell common to hematopoiesis and its in vitro microenvironment: studies of patients with clonal hematopoietic neoplasia. , 1984, Leukemia research.

[40]  P. Quesenberry,et al.  Detection of a human CFC with a high proliferative potential. , 1989, Blood.

[41]  Mary Anne Wheeler,et al.  Stem , 1985 .

[42]  N. Russell,et al.  Comparative study of CD34-positive cells and subpopulations in human umbilical cord blood and bone marrow. , 1993, Bone marrow transplantation.

[43]  A. Miller,et al.  Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production , 1986, Molecular and cellular biology.

[44]  L. Lasky,et al.  Global vascular expression of murine CD34, a sialomucin-like endothelial ligand for L-selectin , 1994 .

[45]  R. Hoffman,et al.  Human CD34+ HLA-DR- bone marrow cells contain progenitor cells capable of self-renewal, multilineage differentiation, and long-term in vitro hematopoiesis. , 1991, Blood cells.

[46]  David A. Williams,et al.  Stem cell factor is encoded at the SI locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor , 1990, Cell.

[47]  J. Till,et al.  Cytological Demonstration of the Clonal Nature of Spleen Colonies Derived from Transplanted Mouse Marrow Cells , 1963, Nature.

[48]  W. Lee,et al.  Phenotypic analysis and characterization of CD34+ cells from normal human bone marrow, cord blood, peripheral blood, and mobilized peripheral blood from patients undergoing autologous stem cell transplantation. , 1994, Clinical immunology and immunopathology.

[49]  I. Bernstein,et al.  Monoclonal antibody 12-8 recognizes a 115-kd molecule present on both unipotent and multipotent hematopoietic colony-forming cells and their precursors , 1986 .

[50]  M. Greaves,et al.  Identification of a membrane glycoprotein associated with haemopoietic progenitor cells. , 1985, Leukemia research.

[51]  B. Dupont,et al.  Graft rejection in recipients of T-cell-depleted HLA-nonidentical marrow transplants for leukemia. Identification of host-derived antidonor allocytotoxic T lymphocytes. , 1987, Transplantation.

[52]  P. Burke,et al.  Successful engraftment of blood derived normal hemopoietic stem cells in chronic myelogenous leukemia. , 1981, Experimental hematology.

[53]  B. Singh,et al.  T cells that recognize peptide sequences of self MHC class II molecules exist in syngeneic mice. , 1991, Journal of immunology.

[54]  C. Dunbar,et al.  Gene transfer into hematopoietic progenitor and stem cells: Progress and problems , 1994, Stem cells.

[55]  J. Till,et al.  A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. , 1961, Radiation research.

[56]  E. Shelton,et al.  Modification of irradiation injury in mice and guinea pigs by bone marrow injections. , 1951, Journal of the National Cancer Institute.

[57]  I. Weissman,et al.  Steel factor influences the distribution and activity of murine hematopoietic stem cells in vivo. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[58]  C. Lange,et al.  Hematopoietic reconstitution of syngeneic mice with a peripheral blood-derived, monoclonal CD34-, Sca-1+, Thy-1(low), c-kit+ stem cell line. , 1999, Journal of hematotherapy & stem cell research.

[59]  P. Simmons,et al.  CD34 expression by stromal precursors in normal human adult bone marrow. , 1991, Blood.

[60]  J. Till,et al.  THE DISTRIBUTION OF COLONY-FORMING CELLS AMONG SPLEEN COLONIES. , 1963, Journal of cellular and comparative physiology.

[61]  M. Fackler,et al.  Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. , 1984, Journal of immunology.

[62]  L. Jacobson,et al.  EFFECT OF SPLEEN PROTECTION ON MORTALITY FOLLOWING X-IRRADIATION , 1949 .

[63]  J. Abkowitz,et al.  Studies in feline long-term marrow culture: hematopoiesis on normal and feline leukemia virus infected stromal cells. , 1992, Blood.

[64]  A. Morley,et al.  OSCILLATORY NATURE OF HEMOPOIESIS. , 1970 .

[65]  N. Russell,et al.  Peripheral blood stem cells as an alternative to marrow for allogeneic transplantation , 1993, The Lancet.

[66]  I. Weissman,et al.  Searching for hematopoietic stem cells: evidence that Thy-1.1lo Lin- Sca-1+ cells are the only stem cells in C57BL/Ka-Thy-1.1 bone marrow , 1992, The Journal of experimental medicine.

[67]  J. Eliason,et al.  Isolation , 2024, Encyclopedia of Database Systems.

[68]  M. Goodell.  CD34+ or CD34−: Does it Really Matter? , 1999 .

[69]  R. Huss.  CD34- stem cells as the earliest precursors of hematopoietic progeny. , 1998, Experimental hematology.

[70]  M. Ogawa,et al.  Human bone marrow CD34- cells engraft in vivo and undergo multilineage expression that includes giving rise to CD34+ cells. , 1998, Experimental hematology.

[71]  Hiromitsu Nakauchi,et al.  Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell , 1996, Science.

[72]  S. Karlsson,et al.  Transduction of nondividing cells using pseudotyped defective high-titer HIV type 1 particles. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[73]  M. W.M,et al.  Lymphoid and Myeloid Differentiation of Single Human CD 34 + , HLA-DR + , CD 38-Hematopoietic Stem Cells , 2002 .

[74]  I. Weissman,et al.  Isolation of two early B lymphocyte progenitors from mouse marrow: A committed Pre-Pre-B cell and a clonogenic Thy-1lo hematopoietic stem cell , 1986, Cell.

[75]  I. Weissman,et al.  The in vitro response of phenotypically defined mouse stem cells and myeloerythroid progenitors to single or multiple growth factors. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[76]  M. Pittenger,et al.  Multilineage potential of adult human mesenchymal stem cells. , 1999, Science.

[77]  I. Bernstein,et al.  Isolation and characterization of a monoclonal antibody that recognizes the human c-kit receptor. , 1992, Blood.

[78]  S. Heimfeld,et al.  Ex vivo expansion of enriched peripheral blood CD34+ progenitor cells by stem cell factor, interleukin-1 beta (IL-1 beta), IL-6, IL-3, interferon-gamma, and erythropoietin , 1993 .

[79]  R. Storb,et al.  Marrow repopulating ability of peripheral blood cells compared to thoracic duct cells. , 1968, Blood.

[80]  Darwin J. Prockop,et al.  Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta , 1999, Nature Medicine.

[81]  I. Weissman,et al.  Proliferation and differentiation of highly enriched mouse hematopoietic stem cells and progenitor cells in response to defined growth factors , 1988, The Journal of experimental medicine.

[82]  H. Deeg,et al.  Differentiation of canine bone marrow cells with hemopoietic characteristics from an adherent stromal cell precursor. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[83]  K. Dorshkind,et al.  Regulation of hemopoiesis by bone marrow stromal cells and their products. , 1990, Annual review of immunology.

[84]  H. Deeg,et al.  Ultrastructural localization of stem cell factor in canine marrow-derived stromal cells. , 1995, Experimental hematology.

[85]  J. Visser,et al.  Purification of pluripotent hemopoietic stem cells: past and present. , 1990, Experimental hematology.

[86]  W. C. Forrester,et al.  High-level beta-globin expression after retroviral transfer of locus activation region-containing human beta-globin gene derivatives into murine erythroleukemia cells. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[87]  R. Nuscher,et al.  Bone marrow transplantation. , 1977, The American journal of nursing.

[88]  I. Weissman,et al.  Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus , 1988, The Journal of experimental medicine.

[89]  J. Kurtzberg,et al.  Hematopoietic reconstitution in a patient with Fanconi's anemia by means of umbilical-cord blood from an HLA-identical sibling. , 1989, The New England journal of medicine.

[90]  S. Ogawa,et al.  Cardiomyocytes can be generated from marrow stromal cells in vitro. , 1999, The Journal of clinical investigation.

[91]  H. Deeg,et al.  Contact- and growth factor-dependent survival in a canine marrow-derived stromal cell line. , 1995, Blood.

[92]  H. Deeg,et al.  Reviews, Notes, and Listings: Impotence: Diagnosis and Management of Male Erectile Dysfunction , 1993, Annals of Internal Medicine.

[93]  R Storb,et al.  Technique for human marrow grafting. , 1970, Blood.

[94]  G. Bonadonna,et al.  Circulation of CD34+ hematopoietic stem cells in the peripheral blood of high-dose cyclophosphamide-treated patients: enhancement by intravenous recombinant human granulocyte-macrophage colony-stimulating factor. , 1989, Blood.

[95]  R. Hoffman,et al.  Long-term generation and expansion of human primitive hematopoietic progenitor cells in vitro. , 1993, Blood.

[96]  J. Nemunaitis,et al.  Simian virus 40-transformed adherent cells from human long-term marrow cultures: cloned cell lines produce cells with stromal and hematopoietic characteristics. , 1987, Blood.

[97]  H. Deeg,et al.  A Guide to Bone Marrow Transplantation , 1988, Springer Berlin Heidelberg.

[98]  K. Foon,et al.  Immunologic classification of leukemia and lymphoma. , 1986, Blood.