Dynamic metabolic changes during fruit maturation in Jatropha curcas L.

To understand how metabolism changes during fruit ripening in Jatropha curcas L., we performed a non-targeted analysis of metabolites in fruit (the pericarp and developing young seeds) from each maturation stage by means of liquid chromatography-Orbitrap-mass spectrometry, which provides m/z data with approximately 2 ppm precision. The chromatographic data were processed using bioinformatics tools. The total number of metabolites detected decreased substantially with fruit maturation. Self-organizing map (SOM) analysis and metabolite annotation of the ions detected suggested that dynamic metabolic changes occur during fruit maturation. All chromatographic data were deposited in databases accessible by the public.

[1]  D. Goodenowe,et al.  Nontargeted metabolome analysis by use of Fourier Transform Ion Cyclotron Mass Spectrometry. , 2002, Omics : a journal of integrative biology.

[2]  K. Becker,et al.  Chemical composition, toxic/antimetabolic constituents, and effects of different treatments on their levels, in four provenances of Jatropha curcas L. from Mexico , 2006 .

[3]  K. Becker,et al.  Phorbol Esters: Structure, Biological Activity, and Toxicity in Animals , 2007, International journal of toxicology.

[4]  S. Kanaya,et al.  Analysis of codon usage diversity of bacterial genes with a self-organizing map (SOM): characterization of horizontally transferred genes with emphasis on the E. coli O157 genome. , 2001, Gene.

[5]  K. Becker,et al.  Nutritional, biochemical, and pharmaceutical potential of proteins and peptides from jatropha: review. , 2010, Journal of agricultural and food chemistry.

[6]  S. Kanaya,et al.  Summary , 1940, Intellectual Property in the Conflict of Laws.

[7]  Ashwani Kumar,et al.  An evaluation of multipurpose oil seed crop for industrial uses (Jatropha curcas L.): A review , 2008 .

[8]  Nozomu Sakurai,et al.  MatchedIonsFinder: A software tool for revising alignment matrices of spectrograms from liquid chromatography-mass spectrometry , 2012 .

[9]  Robert D. Hall,et al.  Plant Metabolomics in a nutshell: Potential and Future Challenges , 2011 .

[10]  M. Hirai,et al.  Integration of transcriptomics and metabolomics for understanding of global responses to nutritional stresses in Arabidopsis thaliana. , 2004, Proceedings of the National Academy of Sciences of the United States of America.