Anatomical substrates of cholinergic‐autonomic regulation in the rat

Acetylcholine (ACh) plays a major role in central autonomic regulation, including the control of arterial blood pressure (AP). Previously unknown neuroanatomic substrates of cholinergic ‐ autonomic control were mapped in this study. Cholinergic perikarya and bouton‐like varicosities were localized by an immunocytochemical method empolying a monoclonal antiserum against choline acetyltransferase (ChAT), the enzyme synthesizing ACh.

[1]  J. A. Ricardo,et al.  Anatomical evidence of direct projections from the nucleus of the solitary tract to the hypothalamus, amygdala, and other forebrain structures in the rat , 1978, Brain Research.

[2]  D. Reis,et al.  Distribution of dopamine‐, noradrenaline‐, and adrenaline‐containing cell bodies in the rat medulla oblongata: Demonstrated by the immunocytochemical localization of catecholamine biosynthetic enzymes , 1982, The Journal of comparative neurology.

[3]  Tohru Hiura.  Salivatory neurons innervate the submandibular and sublingual glands in the rat: horseradish peroxidase study , 1977, Brain Research.

[4]  D. Jacobowitz,et al.  Choline acetyltransferase activity in discrete brain nuclei of DOCA-salt hypertensive rats , 1980, Brain Research.

[5]  M. Palkovits,et al.  Acetylcholine content in different regions of the rat brain , 1978, Brain Research Bulletin.

[6]  D. Reis,et al.  Tonic vasomotor control by the rostral ventrolateral medulla: effect of electrical or chemical stimulation of the area containing C1 adrenaline neurons on arterial pressure, heart rate, and plasma catecholamines and vasopressin , 1984, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[7]  D. Reis,et al.  Organization of central adrenergic pathways: I. Relationships of ventrolateral medullary projections to the hypothalamus and spinal cord , 1987, The Journal of comparative neurology.

[8]  T. Metoki.  Antidromically identified neurohypophysial neurosecretory neurons: effects of hemorrhage, retransfusion and hyperosmolal stimulation on unit activity in the rat , 1976, Brain Research.

[9]  W. Hoffman,et al.  Central Cholinergic Receptors in Cardiovascular and Antidiuretic Effects in Rats , 1979, Clinical and experimental pharmacology & physiology.

[10]  C. Feuerstein,et al.  Immunohistochemical evidence for the coexistence of cholinergic and catecholaminergic phenotypes in neurones of the vagal motor nucleus in the adult rat , 1987, Neuroscience Letters.

[11]  W. Hoffman,et al.  Antidiuretic hormone release and the pressor response to central angiotensin II and cholinergic stimulation , 1977, Neuropharmacology.

[12]  M. Kalia,et al.  Brainstem projections of sensory and motor components of the vagus nerve in the rat , 1982, The Journal of comparative neurology.

[13]  T. Hökfelt,et al.  Evidence for enkephalin immunoreactive neurons in the medulla oblongata projecting to the spinal cord , 1979, Neuroscience Letters.

[14]  G. Paxinos,et al.  The Rat Brain in Stereotaxic Coordinates , 1983 .

[15]  M. Mesulam,et al.  Brain stem projections of sensory and motor components of the vagus complex in the cat: II. Laryngeal, tracheobronchial, pulmonary, cardiac, and gastrointestinal branches , 1980, The Journal of comparative neurology.

[16]  H. Brezenoff.  Cardiovascular responses to intrahypothalamic injections of carbachol and certain cholinesterase inhibitors. , 1972, Neuropharmacology.

[17]  R. Quirion.  Characterization and autoradiographic distribution of hemicholinium‐3 high‐affinity choline uptake sites in mammalian brain , 1987, Synapse.

[18]  W. T. Nickell,et al.  The brain nucleus locus coeruleus: restricted afferent control of a broad efferent network. , 1986, Science.

[19]  A. Levey,et al.  Distribution of cholinergic neurons in rat brain: Demonstrated by the immunocytochemical localization of choline acetyltransferase , 1983, The Journal of comparative neurology.

[20]  A. Goodchild,et al.  Role of ventrolateral medulla in vasomotor regulation: a correlative anatomical and physiological study , 1982, Brain Research.

[21]  A. McDonald,et al.  Cytoarchitecture of the central amygdaloid nucleus of the rat , 1982, The Journal of comparative neurology.

[22]  R. Pásaro,et al.  A morphological study of ambiguus nucleus motoneurons innervating the laryngeal muscles in the rat and cat , 1981, Neuroscience Letters.

[23]  D. Warburton,et al.  Neurochemistry of behaviour. , 1981, British medical bulletin.

[24]  M. Kuhar,et al.  Distribution of muscarinic cholinergic high and low affinity agonist binding sites: A light microscopic autoradiographic study , 1984, Brain Research Bulletin.

[25]  M. Effenberger,et al.  Mapping of cholinoceptive(nicotinoceptive)neurons in the lower brainstem: with special reference to the ventral surface of the medulla. , 1985, Journal of the autonomic nervous system.

[26]  J. Buccafusco,et al.  Cholinergic neurons and the cardiovascular response produced by central injection of substance P in the normotensive rat. , 1986, Life sciences.

[27]  D. Reis,et al.  Projections from the nucleus tractus solitarii to the rostral ventrolateral medulla , 1985, The Journal of comparative neurology.

[28]  D. McCormick,et al.  Two types of muscarinic response to acetylcholine in mammalian cortical neurons. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[29]  T. Gray,et al.  Galanin-like immunoreactivity within amygdaloid and hypothalamic neurons that project to the midbrain central grey in rat , 1987, Neuroscience Letters.

[30]  B. E. Jones,et al.  Atlas of catecholamine perikarya, varicosities and pathways in the brainstem of the cat , 1983, The Journal of comparative neurology.

[31]  J. Buccafusco,et al.  Potentiation of the carotid artery occlusion reflex by a cholinergic system in the posterior hypothalamic nucleus. , 1982, Life sciences.

[32]  D. D. Avery,et al.  Effects of intrahypothalamic injections of adrenergic and cholinergic substances on behavioral thermoregulation and associated skin temperature levels in rats , 1973 .

[33]  G. P. Smith,et al.  Central distribution of subdiaphragmatic vagal branches in the rat , 1988, The Journal of comparative neurology.

[34]  D. Reis,et al.  Anatomical evidence for interactions between catecholamine- and adrenocorticotropin-containing neurons , 1986, Neuroscience.

[35]  C. Saper,et al.  Efferent connections of the parabrachial nucleus in the rat , 1980, Brain Research.

[36]  G. Kreutzberg.  Neuronal dynamics and axonal flow. IV. Blockage of intra-axonal enzyme transport by colchicine. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[37]  I. Koplovitz,et al.  Gastric secretory response of the anesthetized dog after direct chemical stimulation of the supraoptic region , 1977, Experimental Neurology.

[38]  M. Fujiwara,et al.  Possible roles of central cholinergic nicotinic mechanisms in regulation of gastric functions. , 1982, European journal of pharmacology.

[39]  H. Brezenoff,et al.  Cardiovascular control by cholinergic mechanisms in the central nervous system. , 1982, Annual review of pharmacology and toxicology.

[40]  W. Young,et al.  Autoradiographic localization of muscarinic cholinergic receptors in rat brainstem , 1981, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[41]  M. Herkenham,et al.  Mismatches between neurotransmitter and receptor localizations in brain: observations and implications , 1987, Neuroscience.

[42]  A. Levey,et al.  Cholinergic innervation of cortex by the basal forebrain: Cytochemistry and cortical connections of the septal area, diagonal band nuclei, nucleus basalis (Substantia innominata), and hypothalamus in the rhesus monkey , 1983, The Journal of comparative neurology.

[43]  P. Shiromani,et al.  Pontine neuronal response to local cholinergic infusion: Relation to REM sleep , 1986, Brain Research.

[44]  A. Beaudet,et al.  Distribution of acetylcholine and catecholamine neurons in the cat brainstem: A choline acetyltransferase and tyrosine hydroxylase immunohistochemical study , 1987, The Journal of comparative neurology.

[45]  D. Reis,et al.  The role of the solitary and paramedian reticular nuclei in mediating cardiovascular reflex responses from carotid baro‐ and chemoreceptors , 1972, The Journal of physiology.

[46]  A. Goodchild,et al.  Afferent connections and spinal projections of the pressor region in the rostral ventrolateral medulla of the cat. , 1987, Journal of the autonomic nervous system.

[47]  W. Hoffman,et al.  Central cholinergic and noradrenergic stimulation in spontaneously hypertensive rats. , 1978, Journal of Pharmacology and Experimental Therapeutics.

[48]  A. Loewy,et al.  Serotonergic projections from the ventral medulla to the intermediolateral cell column in the rat , 1981, Brain Research.

[49]  C. Saper,et al.  Convergence of autonomic and limbic connections in the insular cortex of the rat , 1982, The Journal of comparative neurology.

[50]  A. Loewy,et al.  Efferent connections of the ventral medulla oblongata in the rat , 1981, Brain Research Reviews.

[51]  C. Saper,et al.  Colocalization of atriopeptin-like immunoreactivity with choline acetyltransferase-and substance P-like immunoreactivity in the pedunculopontine and laterodorsal tegmental nuclei in the rat , 1986, Brain Research.

[52]  J. Chalmers,et al.  Direct projection of catecholamine (presumably dopamine)-containing neurons from hypothalamus to spinal cord , 1979, Neuroscience Letters.

[53]  G. Aghajanian,et al.  Afferent projections to the rat locus coeruleus as determined by a retrograde tracing technique , 1978, The Journal of comparative neurology.

[54]  N. Mizuno,et al.  Parabrachial nucleus neurons projecting to the lower brain stem and the spinal cord. A study in the cat by the Fink-Heimer and the horseradish peroxidase methods , 1980, Experimental Neurology.

[55]  D. Cheney,et al.  Choline acetyltransferase activity and mass fragmentographic measurement of acetylcholine in specific nuclei and tracts of rat brain , 1975, Neuropharmacology.

[56]  Organization of central control of airways. , 1987, Annual review of physiology.

[57]  T. Lovick.  Projections from brainstem nuclei to the nucleus paragigantocellularis lateralis in the cat. , 1986, Journal of the autonomic nervous system.

[58]  J. Coote,et al.  The influence of bulbospinal monoaminergic pathways on sympathetic nerve activity , 1974, The Journal of physiology.

[59]  J. Gnadt,et al.  Cholinergic brainstem mechanisms of REM sleep in the rat , 1986, Brain Research.

[60]  David M. Jacobowitz,et al.  A mapping of the distribution of acetylcholine, choline acetyltransferase and acetylcholinesterase in discrete areas of rat brain , 1978, Brain Research.

[61]  J. Buccafusco,et al.  Hypertension following intrathecal injection of cholinergic agonists in conscious rats: role of endogenous acetylcholine. , 1988, Journal of the autonomic nervous system.

[62]  N. Mizuno,et al.  Localization of parabrachial nucleus neurons projecting to the thalamus or the amygdala in the cat using horseradish peroxidase , 1979, Experimental Neurology.

[63]  T. O'donohue,et al.  Neuropeptide Y innervation of amygdaloid and hypothalamic neurons that project to the dorsal vagal complex in rat , 1986, Peptides.

[64]  D. Reis,et al.  A high density of muscarinic receptors in the rostral ventrolateral medulla of the rat is revealed by correction for autoradiographic efficiency , 1988, Neuroscience Letters.

[65]  J. Ternaux,et al.  Cholinergic neurons in the rat nodose ganglia , 1987, Neuroscience Letters.

[66]  D. Reis,et al.  Reevaluation of projections from the mesencephalic trigeminal nucleus to the medulla and spinal cord: New projections. A combined retrograde and anterograde horseradish peroxidase study , 1982, The Journal of comparative neurology.

[67]  A. Loewy.  Raphe pallidus and raphe obscurus projections to the intermediolateral cell column in the rat , 1981, Brain Research.

[68]  D. Reis,et al.  Phenylethanolamine N-methyltransferase-containing neurons in the rostral ventrolateral medulla. II. Synaptic relationships with GABAergic terminals , 1987, Brain Research.

[69]  H. Yamamura,et al.  Quantitative light microscopic autoradiography of [3H]hemicholinium-3 binding sites in the rat central nervous system: a novel biochemical marker for mapping the distribution of cholinergic nerve terminals , 1985, Brain Research.

[70]  G. Aghajanian,et al.  ACh, substance P and met-enkephalin in the locus coeruleus: pharmacological evidence for independent sites of action. , 1979, European journal of pharmacology.

[71]  D. Reis,et al.  Distribution of neurons containing phenylethanolamine N‐methyltransferase in medulla and hypothalamus of rat , 1985, The Journal of comparative neurology.

[72]  D. Reis,et al.  Projections to the spinal cord from neurons close to the ventral surface of the hindbrain in the rat , 1981, Neuroscience Letters.

[73]  C. Saper,et al.  Pedunculopontine tegmental nucleus of the rat: Cytoarchitecture, cytochemistry, and some extrapyramidal connections of the mesopontine tegmentum , 1987, The Journal of comparative neurology.

[74]  J. E. Vaughn,et al.  Organization and morphological characteristics of cholonergic neurons: an immunocytochemical study with a monoclonal antibody to choline acetyltransferase , 1983, Brain Research.

[75]  D. Reis,et al.  Quantitative distribution of muscarinic receptors and choline acetyltransferase in rat medulla: examination of transmitter-receptor mismatch , 1988, Brain Research.

[76]  F. Eckenstein,et al.  Identification of central cholinergic neurons containing both choline acetyltransferase and acetylcholinesterase and of central neurons containing only acetylcholinesterase , 1983, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[77]  C. Saper,et al.  Specificity of spinal projections from hypothalamic and brainstem areas which innervate sympathetic preganglionic neurons , 1985, Brain Research.

[78]  N. Alessi,et al.  Stress induces supersensitivity of a cholinergic system in rats , 1986, Biological Psychiatry.

[79]  D. Jacobowitz,et al.  Topographic atlas of catecholamine and acetylcholinesterase‐containing neurons in the rat brain. I. Forebrain (telencephalon, diencephalon) , 1974, The Journal of comparative neurology.

[80]  K. Fuxe,et al.  Rat medulla oblongata. II. Dopaminergic, noradrenergic (A1 and A2) and adrenergic neurons, nerve fibers, and presumptive terminal processes , 1985, The Journal of comparative neurology.

[81]  L. Huey,et al.  Central and peripheral cholinesterase inhibition: effects on anterior pituitary and sympathomimetic function , 1986, Psychoneuroendocrinology.

[82]  S. Spencer,et al.  Central modulation of gastric pressure by substance P: a comparison with glutamate and acetylcholine , 1986, Brain Research.

[83]  D. Richter,et al.  Morphology of physiologically identified slowly adapting lung stretch receptor afferents stained with intra‐axonal horseradish peroxidase in the nucleus of the tractus solitarius of the cat. I. A light microscopic analysis , 1985, The Journal of comparative neurology.

[84]  T. Ichikawa,et al.  Organization of choline acetyltransferase-containing structures in the forebrain of the rat , 1986, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[85]  J. Price,et al.  The distribution of choline acetyltransferase in the rat amygdaloid complex and adjacent cortical areas, as determined by quantitative micro‐assay and immunohistochemistry , 1986, The Journal of comparative neurology.

[86]  S Tucek,et al.  Regulation of Acetylcholine Synthesis in the Brain , 1985, Journal of neurochemistry.

[87]  T. Hökfelt,et al.  Neuropeptide Y (NPY)- and FMRFamide neuropeptide-like immunoreactivities in catecholamine neurons of the rat medulla oblongata. , 1983, Acta physiologica Scandinavica.

[88]  H. Kimura,et al.  The central cholinergic system studied by choline acetyltransferase immunohistochemistry in the cat , 1981, The Journal of comparative neurology.

[89]  S. Eliash,et al.  The muscarinic cholinergic receptors in the posterior hypothalamus of hypertensive and normotensive rats. , 1982, European journal of pharmacology.

[90]  J. Axelrod,et al.  Changes in Central Catecholaminergic Neurons in the Spontaneously (Genetic) Hypertensive Rat , 1978, Circulation research.

[91]  R. Norgren,et al.  Central origins of cranial nerve parasympathetic neurons in the rat , 1980, The Journal of comparative neurology.

[92]  Y. Okuma,et al.  Central noradrenergic-cholinergic interaction in regulation of gastric acid secretion in rats. , 1983, Life sciences.

[93]  K. Nakamura.  Influence of sino-aortic baroreceptor denervation on catecholamines, catecholamine-synthesizing enzymes and choline acetyltransferase activity in the brainstem nuclei of the rat. , 1981, Japanese journal of pharmacology.

[94]  S. Brudzyński.  Carbachol-induced agonistic behavior in cats: aggressive or defensive response. , 1981, Acta neurobiologiae experimentalis.

[95]  D. Reis,et al.  Newly identified GABAergic neurons in regions of the ventrolateral medulla which regulate blood pressure , 1985, Brain Research.

[96]  R. Willette,et al.  Cardiovascular control by cholinergic mechanisms in the rostral ventrolateral medulla. , 1984, The Journal of pharmacology and experimental therapeutics.

[97]  H. Thoenen,et al.  Topography of choline acetyltransferase-containing neurons in the forebrain of the rat , 1982, Neuroscience Letters.

[98]  C. Saper,et al.  Direct projections from the A5 catecholamine cell group to the intermediolateral cell column , 1979, Brain Research.

[99]  D. Armstrong,et al.  Adrenaline neurons in the rostral ventrolateral medulla innervate thoracic spinal cord: A combined immunocytochemical and retrograde transport demonstration , 1981, Neuroscience Letters.

[100]  Kiyohisa Takahashi,et al.  Effect of light on the acetylcholine concentrations of the suprachiasmatic nucleus in the rat , 1984, Brain Research.

[101]  K. Soliman,et al.  Effect of stress on the acetylcholinesterase activity of the hypothalamus-pituitary-adrenal axis in the rat. , 1983, Hormone research.

[102]  J. Buccafusco,et al.  Role of Central Cholinergic Neurons in Experimental Hypertension , 1980, Journal of cardiovascular pharmacology.

[103]  D. Reis,et al.  Role of the nucleus parabrachialis in cardiovascular regulation in cat , 1982, Brain Research.

[104]  M. Kuru,et al.  Vesico-constrictor center and vesico-relaxer center in the medulla. , 1959, The Japanese journal of physiology.

[105]  C. Shute,et al.  The ascending cholinergic reticular system: neocortical, olfactory and subcortical projections. , 1967, Brain : a journal of neurology.

[106]  H. Fibiger,et al.  Cholinergic neurons of the laterodorsal tegmental nucleus: Efferent and afferent connections , 1986, The Journal of comparative neurology.

[107]  J. Siegel,et al.  Alterations in blood pressure and REM sleep after pontine carbachol microinfusion , 1986, Experimental Neurology.

[108]  Tetsuro Yamamoto,et al.  Localization of vagal cardioinhibitory preganglionic neurons within rat brain stem , 1979, The Journal of comparative neurology.

[109]  D. Reis,et al.  A role of insular cortex in cardiovascular function , 1987, The Journal of comparative neurology.

[110]  E. Raftery,et al.  Carvedilol for Systemic Hypertension* , 1987, The American journal of cardiology.

[111]  D. Reis,et al.  Adrenaline synthesizing neurons in the rostral ventrolateral medulla: a possible role in tonic vasomotor control , 1983, Brain Research.

[112]  R. Miselis,et al.  The central organization of the vagus nerve innervating the stomach of the rat , 1985, The Journal of comparative neurology.

[113]  H. Fibiger,et al.  A comparison of the distribution of central cholinergic neurons as demonstrated by acetylcholinesterase pharmacohistochemistry and choline acetyltransferase immunohistochemistry , 1983, Brain Research Bulletin.

[114]  L. Hersh,et al.  Distribution of choline acetyltransferase-containing neurons of the hypothalamus , 1987, Brain Research.

[115]  G. Gilad,et al.  Stress-induced activation of the hippocampal cholinergic system and the pituitary-adrenocortical axis , 1985, Brain Research.

[116]  J. Price,et al.  A description of the amygdaloid complex in the rat and cat with observations on intra‐amygdaloid axonal connections , 1978, The Journal of comparative neurology.

[117]  A. Caputi,et al.  Modulatory effect of brain acetylcholine on reflex-induced bradycardia and tachycardia in conscious rats. , 1980, The Journal of pharmacology and experimental therapeutics.

[118]  G. Mogenson,et al.  Factors affecting cardiovascular responses to stimulation of hypothalamus in the rat , 1976, Experimental Neurology.

[119]  J. Rabey,et al.  Dopaminergic modulation of the septo-hippocampal cholinergic system activity under stress. , 1986, Life sciences.

[120]  B. Kapp,et al.  The origin and extent of direct amygdala projections to the region of the dorsal motor nucleus of the vagus and the nucleus of the solitary tract , 1980, Neuroscience Letters.

[121]  D. Reis,et al.  Different Forms of Adrenal Phenylethanolamine N‐Methyltransferase: Species‐Specific Posttranslational Modification , 1982, Journal of neurochemistry.

[122]  K. Kitahama,et al.  Immunohistochemical evidence for the adrenergic medullary longitudinal bundle as a major ascending pathway to the locus coeruleus , 1987, Neuroscience Letters.

[123]  D. Hopkins,et al.  Viscerotopic representation of the upper alimentary tract in the medulla oblongata in the rat: The nucleus ambiguus , 1987, The Journal of comparative neurology.

[124]  E. Kuhn.  Cholinergic and Adrenergic Release Mechanism for Vasopressin in the Male Rat: A Study with Injections of Neurotransmitters and Blocking Agents into the Third Ventricle , 1974 .

[125]  M. Mesulam,et al.  Central cholinergic pathways in the rat: An overview based on an alternative nomenclature (Ch1–Ch6) , 1983, Neuroscience.

[126]  F. Eckenstein,et al.  Mapping of cholinergic neurons associated with rat supraoptic nucleus: Combined immunocytochemical and histochemical identification , 1983, Brain Research Bulletin.

[127]  A. Sved,et al.  Stimulation of the locus coeruleus decreases arterial pressure , 1987, Brain Research.

[128]  C. Saper,et al.  Co-Localization of substance P- and phenylethanolamine-N-methyltransferase-like immunoreactivity in neurons of ventrolateral medulla that project to the spinal cord: Potential role in control of vasomotor tone , 1985, Neuroscience Letters.

[129]  M Goldstein,et al.  Distribution of tyrosine‐hydroxylase‐immunoreactive neurons in the hypothalamus of rats , 1984, The Journal of comparative neurology.

[130]  T. Hökfelt,et al.  Distribution of neuropeptide immunoreactive nerve terminals within the subnuclei of the nucleus of the tractus solitarius of the rat , 1984, The Journal of comparative neurology.

[131]  D. Reis,et al.  Intrinsic γ-aminobutyric acid neurons in the nucleus of the solitary tract and the rostral ventrolateral medulla of the rat: An immunocytochemical and biochemical study , 1985, Neuroscience Letters.

[132]  R. Moore,et al.  Innervation of the thymus gland by brain stem and spinal cord in mouse and rat. , 1981, The American journal of anatomy.

[133]  E. Bystrzycka.  Afferent projections to the dorsal and ventral respiratory nuclei in the medulla oblongata of the cat studied by the horseradish peroxidase technique , 1980, Brain Research.

[134]  H. Yamamura,et al.  Muscarinic antagonist binding site heterogeneity as evidenced by autoradiography after direct labeling with [3H]-QNB and [3H]-pirenzepine. , 1984, Life sciences.

[135]  J. Ciriello,et al.  Lateral hypothalamic and peripheral cardiovascular afferent inputs to ventrolateral medullary neurons , 1985, Brain Research.

[136]  H. Thoenen,et al.  Production of specific antisera and monoclonal antibodies to choline acetyltransferase: characterization and use for identification of cholinergic neurons. , 1982, The EMBO journal.

[137]  B. Morley,et al.  Evidence that cell bodies in the arcuate nucleus of the hypothalamus are not cholinergic. , 1985, Neuroendocrinology.

[138]  D. Reis,et al.  Central control of the circulation by the rostral ventrolateral reticular nucleus: anatomical substrates. , 1989, Progress in brain research.

[139]  J. Ciriello.  Brainstem projections of aortic baroreceptor afferent fibers in the rat , 1983, Neuroscience Letters.

[140]  L. Swanson,et al.  Immunohistochemical localization of neuronal nicotinic receptors in the rodent central nervous system , 1987, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[141]  H. Burton,et al.  Nuclei of the solitary tract: Efferent projections to the lower brain stem and spinal cord of the cat , 1978, The Journal of comparative neurology.

[142]  Henry Lai,et al.  Sodium-dependent, high-affinity choline uptake in hippocampus and frontal cortex of the rat affected by acute restraint stress , 1986, Brain Research.

[143]  W. C. Groat.  Nervous control of the urinary bladder of the cat , 1975, Brain Research.

[144]  M. Palkovits,et al.  Regional distribution of muscarinic cholinergic receptors in rat brain , 1978, Brain Research.

[145]  J. C. Hancock,et al.  Effect of vagotomy on cholinergic parameters in nuclei of rat medulla oblongata , 1985, Brain Research Bulletin.

[146]  S. Ichida,et al.  Total acetylcholine content, and activities of choline acetyltransferase and acetylcholinesterase in brain and duodenum of SART-stressed (repeated cold-stressed) rat. , 1986, Japanese journal of pharmacology.

[147]  D. Beleslin,et al.  Salivation mediated by central M-2 muscarinic receptors in the cat , 1986, Brain Research Bulletin.

[148]  P. Maclean.  Handbook of Drug and Chemical Stimulation of the Brain , 1976 .

[149]  K. Spyer,et al.  Two types of vagal preganglionic motoneurones projecting to the heart and lungs , 1978, The Journal of physiology.

[150]  S. Hilton,et al.  Ventral medullary neurones excited from the hypothalamic and mid-brain defence areas. , 1984, Journal of the autonomic nervous system.

[151]  Cholinergic systems in mammalian brain identified with antibodies against choline acetyltransferase , 1984, Neurochemistry International.

[152]  J. Saavedra,et al.  Catecholamines and phenylethanolamine N-methyltransferase in selected brain nuclei and in the pineal gland of neurogenically hypertensive rats , 1983, Brain Research.

[153]  C. Pirola,et al.  Serotonin mediates cardiovascular responses to acetylcholine, bradykinin, angiotensin II and norepinephrine in the lateral septal area of the rat brain , 1987, Neuropharmacology.

[154]  D. Reis,et al.  Ultrastructural characterization of substance P‐like immunoreactive neurons in the rostral ventrolateral medulla in relation to neurons containing catecholamine‐synthesizing enzymes , 1988, The Journal of comparative neurology.

[155]  T. Hökfelt,et al.  Evidence for a dopaminergic pathway in the rat descending from the A11 cell group to the spinal cord. , 1979, Acta physiologica Scandinavica.

[156]  J. Zimmer,et al.  The architecture and some of the interconnections of the rat's amygdala and lateral periallocortex , 1984, The Journal of comparative neurology.

[157]  H. Ladinsky,et al.  Distribution of muscarinic receptor subtypes in rat brain as determined in binding studies with AF-DX 116 and pirenzepine. , 1987, Life sciences.

[158]  L. Swanson,et al.  Central noradrenergic pathways for the integration of hypothalamic neuroendocrine and autonomic responses. , 1981, Science.

[159]  D. Reis,et al.  Rostral ventrolateral medulla: Selective projections to the thoracic autonomic cell column from the region containing C1 adrenaline neurons , 1984, The Journal of comparative neurology.

[160]  W. Hoffman,et al.  A pressor response to intraventricular injections of carbachol , 1976, Brain Research.

[161]  D. Reis,et al.  Cholinergic regulation of arterial pressure by the C1 area of the rostral ventrolateral medulla , 1989, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[162]  Brudzyński Sm.  Growling component of vocalization as a quantitative index of carbachol-induced emotional-defensive response in cats. , 1981 .

[163]  F. Eldridge,et al.  Role of ventrolateral medulla in regulation of respiratory and cardiovascular systems. , 1986, Journal of applied physiology.

[164]  P. Hand,et al.  Subfornical organ efferents to neural systems for control of body water. , 1979, Science.

[165]  D. Beleslin,et al.  A dose response study of aggressive behavioral effects of intracerebroventricular injections of carbachol in cats , 1986, Physiology & Behavior.

[166]  N. W. Pedigo,et al.  Muscarinic cholinergic receptors in area postrema and brainstem areas regulating emesis , 1985, Brain Research Bulletin.

[167]  Péter Kása.  The cholinergic systems in brain and spinal cord , 1986, Progress in Neurobiology.

[168]  M. Tohyama,et al.  Spinal projections from the lower brain stem in the cat as demonstrated by the horseradish peroxidase technique. II. projections from the dorsolateral pontine tegmentum and raphe nuclei , 1979, Brain Research.

[169]  B. Wainer,et al.  Evidence for the coexistence of acetylcholine and enkephalin in the sympathetic preganglionic neurons of rats , 1985, Brain Research.

[170]  Teiji Yamamoto,et al.  Identification of the inferior salivatory nucleus in the cat as studied by HRP bathings of the transected glossopharyngeal nerve root , 1979, Neuroscience Letters.

[171]  M. Palkovits,et al.  CHOLINE ACETYLTRANSFERASE CONTENT IN DISCRETE REGIONS OF THE RAT BRAIN STEM , 1975, Journal of neurochemistry.

[172]  R. Nieuwenhuys,et al.  The medial forebrain bundle of the rat. I. General introduction , 1982, The Journal of comparative neurology.

[173]  W. Lovenberg,et al.  Effect of tissue norepinephrine depletion by 6-hydroxydopamine on blood pressure in spontaneously hypertensive rats. , 1972, European journal of pharmacology.

[174]  A. Dahlström.  Effect of colchicine on transport of amine storage granules in sympathetic nerves of rat. , 1968, European journal of pharmacology.

[175]  M. Mesulam,et al.  Atlas of cholinergic neurons in the forebrain and upper brainstem of the macaque based on monoclonal choline acetyltransferase immunohistochemistry and acetylcholinesterase histochemistry , 1984, Neuroscience.

[176]  W. Ganong,et al.  The role of catecholamines and acetylcholine in the regulation of endocrine function. , 1974, Life sciences.

[177]  J. Buccafusco,et al.  The hypertensive response to injection of physostigmine into the hypothalamus of the unanesthetized rat. , 1978, Clinical and experimental hypertension.

[178]  H. Kimura,et al.  The cholinergic system of the human hindbrain studied by choline acetyltransferase immunohistochemistry and acetylcholinesterase histochemistry , 1986, Brain Research.

[179]  G. L. Gebber,et al.  Lateral tegmental field neurons of cat medulla: a potential source of basal sympathetic nerve discharge. , 1985, Journal of neurophysiology.

[180]  R. Willette,et al.  Activation of cholinergic mechanisms in the medulla oblongata reverse intravenous opioid-induced respiratory depression. , 1987, The Journal of pharmacology and experimental therapeutics.

[181]  E. Jones.  Distribution patterns of individual medial lemniscal axons in the ventrobasal complex of the monkey thalamus , 1983, The Journal of comparative neurology.

[182]  P. Clarke,et al.  Nicotinic binding in rat brain: autoradiographic comparison of [3H]acetylcholine, [3H]nicotine, and [125I]-alpha-bungarotoxin , 1985, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[183]  D. Jacobowitz,et al.  Changes in central cholinergic neurons in the spontaneously hypertensive rat , 1980, Brain Research.

[184]  R. Timms,et al.  Ventral medullary relay neurones in the pathway from the defence areas of the cat and their effect on blood pressure. , 1983, The Journal of physiology.

[185]  Clifford B. Saper,et al.  Subnuclear organization of the efferent connections of the parabrachial nucleus in the rat , 1984, Brain Research Reviews.

[186]  D. Reis,et al.  Ultrastructural localization of choline acetyltransferase in the rat rostral ventrolateral medulla: evidence for major synaptic relations with non-catecholaminergic neurons , 1989, Brain Research.

[187]  J. Feldman,et al.  Central coordination of respiratory and cardiovascular control in mammals. , 1988, Annual Review of Physiology.

[188]  G. Raisman,et al.  Muscarinic receptors in the central nervous system of the rat. II. Distribution of binding of [3H]propylbenzilylcholine mustard in the midbrain and hindbrain , 1979, Brain Research Reviews.

[189]  J. Buccafusco,et al.  Pharmacological study of a cholinergic mechanism within the rat posterior hypothalamic nucleus which mediates a hypertensive response , 1979, Brain Research.

[190]  M. Hirano,et al.  Location of motoneurons supplying the cricopharyngeal muscle in the cat studied by means of the horseradish peroxidase method , 1980, Neuroscience Letters.

[191]  J. Buccafusco,et al.  Spinal cholinergic neurons and the expression of morphine withdrawal symptoms in the rat , 1987, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[192]  R. Friedman,et al.  Participation of the Central Cholinergic System in Blood Pressure Regulation in the Dahl Rat Model of Essential Hypertension , 1983, Journal of Cardiovascular Pharmacology.

[193]  M. Tohyama,et al.  Descending projection of the nucleus tegmentalis latero-dorsalis to the spinal cord; studied by the horseradish peroxidase method following 6-hydroxy-DOPA administration , 1978, Neuroscience Letters.

[194]  A. Philippu.  Involvement of cholinergic systems of the brain in the central regulation of cardiovascular functions. , 1981, Journal of autonomic pharmacology.

[195]  Donald J. Reis,et al.  Quantitative immunocytochemistry of tyrosine hydroxylase in rat brain. II. Variations in the amount of tyrosine hydroxylase among individual neurons of the locus coeruleus in relationship to neuronal morphology and topography , 1982, Brain Research.

[196]  D. Reis,et al.  Aromaticl-amino acid decar☐ylase in the rat brain: Immunocytochemical localization in neurons of the brain stem , 1984, Neuroscience.

[197]  T. Gray,et al.  Evidence for corticotropin‐releasing factor, neurotensin, and somatostatin in the neural pathway from the central nucleus of the amygdala to the parabrachial nucleus , 1985, The Journal of comparative neurology.

[198]  C. Altar,et al.  [3H]vesamicol binding in brain: Autoradiographic distribution, pharmacology, and effects of cholinergic lesions , 1988, Synapse.

[199]  M. Er̀rington,et al.  Projections to the ventral surface of the cat brainstem demonstrated by horseradish peroxidase , 1979, Neuroscience Letters.

[200]  C. Saper,et al.  Descending projections from the pontine micturition center , 1979, Brain Research.

[201]  G. Handelmann,et al.  Choline acetyltransferase activity in the nucleus tractus solitarius: Regulation by the afferent vagus nerve , 1983, Brain Research Bulletin.