Human Blood Cell Sensing with Platinum Black Electroplated Impedance Sensor

AC impedance sensing is an important method for biological cell analysis in flow cytometry. For micro impedance cell sensors, downsizing electrodes increases the double layer impedance of the metal-electrolyte interface, thus leaves no sensing zone in frequency domain and reduces the sensitivity significantly. We proposed using platinum black electroplated electrodes to solve the problem. In this paper, using this technique we demonstrated human blood cell sensing with high signal to noise ratio.

[1]  T. Pajkossy,et al.  Tafel current at fractal electrodes: Connection with admittance spectra , 1990 .

[2]  H. Oka,et al.  A new planar multielectrode array for extracellular recording: application to hippocampal acute slice , 1999, Journal of Neuroscience Methods.

[3]  Honggu Chun,et al.  Cytometry and velocimetry on a microfluidic chip using polyelectrolytic salt bridges. , 2005, Analytical chemistry.

[4]  S. Gawad,et al.  Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing. , 2001, Lab on a chip.

[5]  Carl A. Marrese,et al.  Preparation of strongly adherent platinum black coatings , 1987 .

[6]  Pasqualina M. Sarro,et al.  Integrated Coulter counter based on 2-dimensional liquid aperture control , 2004 .

[7]  Y. Tai,et al.  The neurochip: a new multielectrode device for stimulating and recording from cultured neurons , 1999, Journal of Neuroscience Methods.

[8]  Gregory T. A. Kovacs,et al.  Cell-based sensor microelectrode array characterized by imaging x-ray photoelectron spectroscopy, scanning electron microscopy, impedance measurements, and extracellular recordings , 1998 .

[9]  Makoto Ishida,et al.  A sensor for blood cell counter using MEMS technology , 2002 .

[10]  Richard D. Rabbitt,et al.  Electric impedance spectroscopy using microchannels with integrated metal electrodes , 1999 .

[11]  Urban Seger,et al.  Dielectric spectroscopy in a micromachined flow cytometer: theoretical and practical considerations. , 2004, Lab on a chip.

[12]  S. B. Mckenzie,et al.  Clinical Laboratory Hematology , 2006 .

[13]  Hermann Sandmaier,et al.  Absorbing layers for thermal infrared detectors , 1992 .

[14]  Koji Asami,et al.  Characterization of heterogeneous systems by dielectric spectroscopy , 2002 .