论文信息 - Preparation of Large Calcified Bone Sections for Fluorescence Histomorphometry

Preparation of Large Calcified Bone Sections for Fluorescence Histomorphometry

Abstract Fluorescent bone markers provide a method for observing the dynamics of bone apposition and resorption. Such a tool should be useful in the evaluation of orthopedic surgical techniques; however, the processing of the large bone sections for analysis can be problematic. We have developed a method for labeling and processing large calcified bone sections. Using a triple fluorescent labeling protocol we are able to measure bone apposition rate between different surgical procedures. We are also able to qualitatively determine the time of re-vascularization within the site of grafted bone. This technique can supplement radiography and biomechanics in assessing orthopedic surgical approaches. (The J Histotechnol 22:93, 1999)

V. Kalscheur | T. Zdeblick | M. Cooke | David Wilson

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