Role of CCAA Nucleotide Repeats in Regulation of Hemoglobin and Hemoglobin-Haptoglobin Binding Protein Genes ofHaemophilus influenzae

ABSTRACT Haemophilus influenzae utilizes hemoglobin and hemoglobin-haptoglobin as heme sources. The H. influenzaehemoglobin- and hemoglobin-haptoglobin binding protein genes,hgpA, hgpB, and hgpC, contain lengths of tetrameric CCAA repeats. Using an hgpA-lacZtranslational gene fusion, we demonstrate phase-variable expression oflacZ associated with alteration in the length of the CCAA repeat region.

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