Protein adsorption on functionalized multiwalled carbon nanotubes with amino‐cyclodextrin

A novel amino-cyclodextrin was synthesized, and it was covalently attached to multiwalled carbon nanotubes (MWNTs). The functionalized MWNTs (f-MWNTs) have very good aqueous dispersibility. Bovine serum albumin (BSA) was adsorbed onto f-MWNTs through noncovalent interactions, including the hydrophobic interaction of the residues of BSA with the wall of MWNT and the guest–host interaction of the residues with the cyclodextrin (CD) moieties of f-MWNTs. The ultraviolet–visible (UV–vis) absorption of the f-MWNT-BSA hybrid was measured with UV–vis spectrometer, and the absorbance can be described well with the Beer–Lambert law. The X-ray diffraction patterns have indicated that the crystalline form of BSA has been changed after the adsorption of BSA on f-MWNTs. The circular dichroism spectra have shown that a high percentage of a-helical content can be retained for BSA adsorbed on f-MWNTs. The results also indicate that the change of secondary structure of BSA is mainly due to the hydrophobic interaction of the residues of BSA with the wall of f-MWNT, whereas the secondary structure is much less affected by the interaction of the CD moieties with BSA. V C 2011 American Institute of Chemical Engineers AIChE J, 57: 3507–3513, 2011

[1]  D. Henthorn,et al.  Synthesis of PEGylated single wall carbon nanotubes by a photoinitiated graft from polymerization , 2010 .

[2]  R. Macgregor,et al.  Origins of pressure-induced protein transitions. , 2009, Journal of molecular biology.

[3]  Sea-Fue Wang,et al.  Adsorption of ciprofloxacin and its role for stabilizing multi-walled carbon nanotubes and characterization , 2009 .

[4]  Hongyu Zhou,et al.  Functionalized carbon nanotubes specifically bind to alpha-chymotrypsin's catalytic site and regulate its enzymatic function. , 2009, Nano letters.

[5]  P. Das,et al.  Superior activity of structurally deprived enzyme-carbon nanotube hybrids in cationic reverse micelles. , 2009, Langmuir : the ACS journal of surfaces and colloids.

[6]  K. Kuwata,et al.  Critical region for amyloid fibril formation of mouse prion protein: unusual amyloidogenic properties of the helix 2 peptide. , 2008, Biochemistry.

[7]  Yuan Gao,et al.  Covalent immobilization of proteins on carbon nanotubes using the cross-linker 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide--a critical assessment. , 2008, Bioconjugate chemistry.

[8]  Yongsheng Chen,et al.  Covalently β-cyclodextrin modified single-walled carbon nanotubes: a novel artificial receptor synthesized by ‘click’ chemistry , 2008 .

[9]  Wei Liu,et al.  Protein Binding by Functionalized Multiwalled Carbon Nanotubes Is Governed by the Surface Chemistry of Both Parties and the Nanotube Diameter , 2008 .

[10]  M. Yumura,et al.  Selectivity of water-soluble proteins in single-walled carbon nanotube dispersions , 2006 .

[11]  Y. Takashima,et al.  Self-threading of a poly(ethylene glycol) chain in a cyclodextrin-ring: control of the exchange dynamics by chain length. , 2006, Journal of the American Chemical Society.

[12]  Laura A. Sowards,et al.  Direct measurements of interactions between polypeptides and carbon nanotubes. , 2006, The journal of physical chemistry. B.

[13]  Ravi S Kane,et al.  Increasing protein stability through control of the nanoscale environment. , 2006, Langmuir : the ACS journal of surfaces and colloids.

[14]  M. Prato,et al.  Chemistry of carbon nanotubes. , 2006, Chemical reviews.

[15]  D. P. Anderson,et al.  Hierarchical morphology of carbon single-walled nanotubes during sonication in an aliphatic diamine , 2005 .

[16]  Alan Gelperin,et al.  DNA-decorated carbon nanotubes for chemical sensing , 2005, Nano letters.

[17]  J. Coleman,et al.  Biomolecules as selective dispersants for carbon nanotubes , 2005 .

[18]  Guodong Liu,et al.  Multiple enzyme layers on carbon nanotubes for electrochemical detection down to 80 DNA copies. , 2005, Analytical chemistry.

[19]  M. Romero,et al.  Immobilization of β-Glucosidase on carbon nanotubes , 2005 .

[20]  Ravi S Kane,et al.  Structure and function of enzymes adsorbed onto single-walled carbon nanotubes. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[21]  K. Murayama,et al.  Heat-induced secondary structure and conformation change of bovine serum albumin investigated by Fourier transform infrared spectroscopy. , 2004, Biochemistry.

[22]  D. Otzen,et al.  Structural background of cyclodextrin-protein interactions. , 2003, Protein engineering.

[23]  M. Shim,et al.  Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[24]  Haiqing Peng,et al.  Sidewall Amino-Functionalization of Single-Walled Carbon Nanotubes through Fluorination and Subsequent Reactions with Terminal Diamines , 2003 .

[25]  D. Otzen,et al.  Structural basis for cyclodextrins' suppression of human growth hormone aggregation , 2002, Protein science : a publication of the Protein Society.

[26]  M. Shim,et al.  Functionalization of Carbon Nanotubes for Biocompatibility and Biomolecular Recognition , 2002 .

[27]  J. Tour,et al.  Highly Functionalized Carbon Nanotubes Using in Situ Generated Diazonium Compounds , 2001 .

[28]  S. Ogawa,et al.  Cycloamylose as an efficient artificial chaperone for protein refolding , 2000, FEBS letters.

[29]  H. Wen,et al.  Comparison of Perturbed Hard-Sphere-Chain Theory with Statistical Associating Fluid Theory for Square-Well Fluids , 2000 .

[30]  T. Ebbesen,et al.  Helical Crystallization of Proteins on Carbon Nanotubes: A First Step towards the Development of New Biosensors. , 1999, Angewandte Chemie.

[31]  Robert Bittman,et al.  An improved synthesis of 6-O-monotosyl-6-deoxy-β-cyclodextrin , 1998 .

[32]  Alexander M. Klibanov,et al.  On Protein Denaturation in Aqueous−Organic Mixtures but Not in Pure Organic Solvents , 1996 .

[33]  A. Cooper,et al.  Energetics of protein-cyclodextrin interactions , 1996 .

[34]  A. Sharma,et al.  Cyclodextrins as protein folding aids. , 1995, Biochemical and biophysical research communications.