Relationship between serum levels of miR-204 and clinical features of patients with lumbar disc herniation - an analysis based on 1,589 cases

This study aims to investigate the relationship between serum miR-204 and clinical features of patients with lumbar disc herniation (LDH). Clinical data of 1589 LDH patients were retrospectively analyzed. There were 953 patients (group A) with a duration of disease shorter than 3 months, and another 636 patients (group B) with a duration of disease longer than 3 months. A total of 300 healthy volunteers were also selected in the same period. Results showed that there was no significant difference in miR-204 expression between males and females (p>0.05). No significant differences in miR-204 expression level were found among different ages (p>0.05). miR-204 in patients with LHD at or below the fourth lumbar vertebrae was significantly higher than those in patients with LHD above the fourth lumbar vertebrae (p<0.05). miR-204 in 1485 patients with abnormal lumbar spine activity was higher than those in healthy controls (p<0.05). miR-204 expression levels was higher in patients with low back pain than those in patients without low back pain. Among patients with low back pain, miR-204 level in patients with spasm-like waist pain was significantly higher than that in patients with persistent dull low back pain (p<0.05). miR-204 increased with the increase in degree of limited walking (p<0.05). There was no significant difference in miR-204 expression level between patients with bilateral lower limb symptoms and patients with unilateral lower limb symptoms (p>0.05), while miR-204 was significantly higher in patients with lower limb symptoms than that in patients without the symptoms (p<0.05). miR-204 was correlated with knee tendon reflex, heel tendon reflex and degree of straight leg raise abnormality. miR-204 was significantly decreased in patients with improvement after treatment. miR-204 level was closely related to the clinical characteristics of LDH. miR-204 may potentially serve as a prognostic marker for patients with LDH.

[1]  Xueqiang Wang,et al.  Surgical versus non-operative treatment for lumbar disc herniation: a systematic review and meta-analysis , 2018, Clinical rehabilitation.

[2]  J. Ouyang,et al.  SNP rs3202538 in 3′UTR region of ErbB3 regulated by miR-204 and miR-211 promote gastric cancer development in Chinese population , 2017, Cancer Cell International.

[3]  Hong Lu,et al.  Value of a new pathological classification of lumbar intervertebral disc herniation based on transforaminal endoscopic observations , 2017, Experimental and therapeutic medicine.

[4]  C. Shih,et al.  The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death , 2016, Neuropharmacology.

[5]  S. Ohtori,et al.  Diffusion tensor imaging of radiculopathy in patients with lumbar disc herniation: preliminary results. , 2016, The bone & joint journal.

[6]  A. Dower,et al.  Surgical management of recurrent lumbar disc herniation and the role of fusion , 2016, Journal of Clinical Neuroscience.

[7]  Wanli Jing,et al.  MicroRNA‐93 regulates collagen loss by targeting MMP3 in human nucleus pulposus cells , 2015, Cell proliferation.

[8]  Lianyong Liu,et al.  MiR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma. , 2015, Biochemical and biophysical research communications.

[9]  D. Hooper,et al.  Five and Ten Year Follow-up on Intradiscal Ozone Injection for Disc Herniation , 2014, International Journal of Spine Surgery.

[10]  D. Plas,et al.  TRPM3 and miR-204 establish a regulatory circuit that controls oncogenic autophagy in clear cell renal cell carcinoma. , 2014, Cancer cell.

[11]  J. Lurie,et al.  Surgical versus nonoperative treatment: how do we choose the right approach to lumbar disk herniation? , 2014, Pain management.

[12]  B. Liu,et al.  Comparative analysis of serum proteomes: Identification of proteins associated with sciatica due to lumbar intervertebral disc herniation. , 2014, Biomedical reports.

[13]  Minhua Wu,et al.  Down‐regulation of miRNA‐204 by LMP‐1 enhances CDC42 activity and facilitates invasion of EBV‐associated nasopharyngeal carcinoma cells , 2014, FEBS letters.

[14]  W. Wu,et al.  MicroRNA-10b Promotes Nucleus Pulposus Cell Proliferation through RhoC-Akt Pathway by Targeting HOXD10 in Intervetebral Disc Degeneration , 2013, PloS one.

[15]  S. Rajasekaran,et al.  ISSLS Prize Winner: The Anatomy of Failure in Lumbar Disc Herniation An In Vivo, Multimodal, Prospective Study of 181 Subjects , 2013, Spine.

[16]  Lihua Zhang,et al.  MiR-204 down regulates SIRT1 and reverts SIRT1-induced epithelial-mesenchymal transition, anoikis resistance and invasion in gastric cancer cells , 2013, BMC Cancer.

[17]  M. Adams,et al.  Intervertebral disc degeneration: evidence for two distinct phenotypes , 2012, Journal of anatomy.

[18]  R. Stallings,et al.  MicroRNA-204 increases sensitivity of neuroblastoma cells to cisplatin and is associated with a favourable clinical outcome , 2012, British Journal of Cancer.

[19]  Lawrence A. Delasotta,et al.  The impact of epidural steroid injections on the outcomes of patients treated for lumbar disc herniation: a subgroup analysis of the SPORT trial. , 2012, The Journal of bone and joint surgery. American volume.

[20]  L. Manchikanti,et al.  Effect of fluoroscopically guided caudal epidural steroid or local anesthetic injections in the treatment of lumbar disc herniation and radiculitis: a randomized, controlled, double blind trial with a two-year follow-up. , 2012, Pain physician.

[21]  James N Weinstein,et al.  Surgical Versus Nonoperative Treatment for Lumbar Disc Herniation: Eight-Year Results for the Spine Patient Outcomes Research Trial , 2008, Spine.

[22]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[23]  Charles H. Cho,et al.  An evidence-based clinical guideline for the diagnosis and treatment of lumbar disc herniation with radiculopathy. , 2014, The spine journal : official journal of the North American Spine Society.

[24]  I. Shapiro,et al.  Role of cytokines in intervertebral disc degeneration: pain and disc content , 2014, Nature Reviews Rheumatology.

[25]  Xiong Guo,et al.  A controlled case study of the relationship between environmental risk factors and apoptotic gene polymorphism and lumbar disc herniation. , 2013, The American journal of pathology.