A one-step construction of adenovirus (OSCA) system using the Gibson DNA Assembly technology

[1]  Jian Yang,et al.  Argonaute (AGO) proteins play an essential role in mediating BMP9-induced osteogenic signaling in mesenchymal stem cells (MSCs) , 2021, Genes & diseases.

[2]  T. He,et al.  BMP9‐initiated osteogenic/odontogenic differentiation of mouse tooth germ mesenchymal cells (TGMCS) requires Wnt/β‐catenin signalling activity , 2021, Journal of cellular and molecular medicine.

[3]  T. He,et al.  Long non-coding RNA (LncRNA) HOTAIR regulates BMP9-induced osteogenic differentiation by targeting the proliferation of mesenchymal stem cells (MSCs) , 2021, Aging.

[4]  T. He,et al.  Modeling colorectal tumorigenesis using the organoids derived from conditionally immortalized mouse intestinal crypt cells (ciMICs) , 2021, Genes & diseases.

[5]  T. He,et al.  FAMSi: A Synthetic Biology Approach to the Fast Assembly of Multiplex siRNAs for Silencing Gene Expression in Mammalian Cells , 2020, Molecular therapy. Nucleic acids.

[6]  Daniel A. Hu,et al.  SATB2: A versatile transcriptional regulator of craniofacial and skeleton development, neurogenesis and tumorigenesis, and its applications in regenerative medicine , 2020, Genes & diseases.

[7]  T. He,et al.  Blockade of IGF/IGF-1R signaling axis with soluble IGF-1R mutants suppresses the cell proliferation and tumor growth of human osteosarcoma. , 2020, American journal of cancer research.

[8]  T. He,et al.  Development of a simplified and inexpensive RNA depletion method for plasmid DNA purification using size selection magnetic beads (SSMBs) , 2020, Genes & diseases.

[9]  T. He,et al.  Leptin Potentiates BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells Through the Activation of JAK/STAT Signaling. , 2020, Stem cells and development.

[10]  T. He,et al.  lncRNA Rmst acts as an important mediator of BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs) by antagonizing Notch-targeting microRNAs , 2019, Aging.

[11]  T. He,et al.  Highly expressed BMP9/GDF2 in postnatal mouse liver and lungs may account for its pleiotropic effects on stem cell differentiation, angiogenesis, tumor growth and metabolism , 2019, Genes & diseases.

[12]  Elliot S. Bishop,et al.  The wonders of BMP9: From mesenchymal stem cell differentiation, angiogenesis, neurogenesis, tumorigenesis, and metabolism to regenerative medicine , 2019, Genes & diseases.

[13]  B. Liu,et al.  A simplified system for the effective expression and delivery of functional mature microRNAs in mammalian cells , 2019, Cancer Gene Therapy.

[14]  B. Liu,et al.  Transcriptomic landscape regulated by the 14 types of bone morphogenetic proteins (BMPs) in lineage commitment and differentiation of mesenchymal stem cells (MSCs) , 2019, Genes & diseases.

[15]  A. M. Ismail,et al.  Rapid Construction of a Replication-Competent Infectious Clone of Human Adenovirus Type 14 by Gibson Assembly , 2018, Viruses.

[16]  Yang Yu,et al.  ☆DNA assembly technique simplifies the construction of infectious clone of fowl adenovirus. , 2018, Journal of virological methods.

[17]  Bo Zhang,et al.  Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference , 2018, Genes & diseases.

[18]  T. He,et al.  CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs) , 2017, Oncotarget.

[19]  Wenli Zhang,et al.  Recent Advances in Preclinical Developments Using Adenovirus Hybrid Vectors. , 2017, Human gene therapy.

[20]  L. Seymour,et al.  Oncolytic adenovirus expressing bispecific antibody targets T‐cell cytotoxicity in cancer biopsies , 2017, EMBO molecular medicine.

[21]  T. He,et al.  BMP9 induces osteogenesis and adipogenesis in the immortalized human cranial suture progenitors from the patent sutures of craniosynostosis patients , 2017, Journal of cellular and molecular medicine.

[22]  T. He,et al.  Engineering the Rapid Adenovirus Production and Amplification (RAPA) Cell Line to Expedite the Generation of Recombinant Adenoviruses , 2017, Cellular Physiology and Biochemistry.

[23]  Chao Yang,et al.  Adenovirus-mediated gene delivery: Potential applications for gene and cell-based therapies in the new era of personalized medicine , 2017, Genes & diseases.

[24]  T. He,et al.  Noncanonical Wnt signaling plays an important role in modulating canonical Wnt-regulated stemness, proliferation and terminal differentiation of hepatic progenitors , 2017, Oncotarget.

[25]  Maryam K. Mohammed,et al.  A thermoresponsive polydiolcitrate-gelatin scaffold and delivery system mediates effective bone formation from BMP9-transduced mesenchymal stem cells , 2016, Biomedical materials.

[26]  V. Mouly,et al.  Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in DMD muscle cell populations , 2016, Nucleic acids research.

[27]  T. He,et al.  A Simplified and Versatile System for the Simultaneous Expression of Multiple siRNAs in Mammalian Cells Using Gibson DNA Assembly , 2014, PloS one.

[28]  X. Chen,et al.  Overexpression of Ad5 precursor terminal protein accelerates recombinant adenovirus packaging and amplification in HEK-293 packaging cells , 2014, Gene Therapy.

[29]  T. He,et al.  Adenovirus-Mediated Gene Transfer in Mesenchymal Stem Cells Can Be Significantly Enhanced by the Cationic Polymer Polybrene , 2014, PloS one.

[30]  T. He,et al.  Targeting BMP9-promoted human osteosarcoma growth by inactivation of notch signaling. , 2014, Current cancer drug targets.

[31]  F. Lienert,et al.  Synthetic biology in mammalian cells: next generation research tools and therapeutics , 2014, Nature Reviews Molecular Cell Biology.

[32]  D. G. Gibson,et al.  Enzymatic Assembly of Overlapping DNA Fragments , 2011, Methods in Enzymology.

[33]  T. He,et al.  Insulin-like Growth Factor 2 (IGF-2) Potentiates BMP-9-Induced Osteogenic Differentiation and Bone Formation , 2010, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[34]  D. G. Gibson,et al.  Enzymatic assembly of DNA molecules up to several hundred kilobases , 2009, Nature Methods.

[35]  K. Kinzler,et al.  A protocol for rapid generation of recombinant adenoviruses using the AdEasy system , 2007, Nature Protocols.

[36]  J. Szatkowski,et al.  Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery , 2004, Gene Therapy.

[37]  T. He,et al.  Adenoviral vector-mediated gene transfer for human gene therapy. , 2001, Current gene therapy.

[38]  F. Graham,et al.  High-efficiency Cre/loxp based system for construction of adenovirus vectors , 1999 .

[39]  M. Kay,et al.  Efficient construction of a recombinant adenovirus vector by an improved in vitro ligation method. , 1998, Human gene therapy.

[40]  M. Kitamura,et al.  Construction of adenovirus vectors through Cre-lox recombination , 1997, Journal of virology.

[41]  M. Mehtali,et al.  Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli , 1996, Journal of virology.

[42]  F. Graham,et al.  Methods for construction of adenovirus vectors , 1995, Molecular biotechnology.

[43]  F. Spencer,et al.  Efficient manipulation of the human adenovirus genome as an infectious yeast artificial chromosome clone. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[44]  T. He,et al.  Canonical Wnt signaling acts synergistically on BMP9-induced osteo/odontoblastic differentiation of stem cells of dental apical papilla (SCAPs). , 2015, Biomaterials.

[45]  R. Crystal.  Adenovirus: the first effective in vivo gene delivery vector. , 2014, Human gene therapy.

[46]  J. Szatkowski,et al.  Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). , 2003, The Journal of bone and joint surgery. American volume.

[47]  K. Kinzler,et al.  A simplified system for generating recombinant adenoviruses. , 1998, Proceedings of the National Academy of Sciences of the United States of America.