Biological effects of narrow band pulsed electric fields

This paper describes the process of narrow band pulsed electric fields (NPEFs) and its effect on mammalian cells. The NPEF consists of a pulse modulated sinusoidal wave (PMSW), which allows delivery of well-defined electric fields in terms of frequency, field strength and deposition energy to the biological systems. 100 mus long sinusoidal electric fields with a frequency of 0.02, 2 or 50 MHz and field strengths of up to 2 kV/cm are applied to CHO cells with variation in the DNA density in the cells investigated by means of Acridine Orange assay. The experiments indicate that 50 MHz fields cause DNA degradation without cell membrane defects, while 0.02 MHz fields lead to an increase in membrane permeability which is similar to the effect known as electroporation. The intermediate frequency of 2 MHz influences both the membrane and DNA. It is demonstrated that the MHz range narrowband electric fields with the amplitude level of 1 kV/cm cause intracellular effects in mammalian cells.

[1]  Michael R. Murphy,et al.  Low-intensity millimeter waves as a novel therapeutic modality , 1999 .

[2]  K. Schoenbach,et al.  Differential effects in cells exposed to ultra-short, high intensity electric fields: cell survival, DNA damage, and cell cycle analysis. , 2003, Mutation research.

[3]  Laura Marcu,et al.  Calcium bursts induced by nanosecond electric pulses. , 2003, Biochemical and biophysical research communications.

[4]  Kenneth R Foster,et al.  Modeling thermal responses in human subjects following extended exposure to radiofrequency energy , 2004, Biomedical engineering online.

[5]  Juergen F Kolb,et al.  Leukemic cell intracellular responses to nanosecond electric fields. , 2004, Biochemical and biophysical research communications.

[6]  K. Schoenbach,et al.  Bioelectrics-new applications for pulsed power technology , 2001, PPPS-2001 Pulsed Power Plasma Science 2001. 28th IEEE International Conference on Plasma Science and 13th IEEE International Pulsed Power Conference. Digest of Papers (Cat. No.01CH37251).

[7]  K. H. Schoenbach,et al.  Effects of submicrosecond, high intensity pulsed electric fields on living cells - intracellular electromanipulation , 2003 .

[8]  P. Gessner,et al.  Electromanipulation of mammalian cells: fundamentals and application , 2000 .

[9]  Ulrich Zimmermann,et al.  Dielectric Breakdown of Cell Membranes , 1974 .

[10]  K. Schoenbach,et al.  Intracellular effect of ultrashort electrical pulses , 2001, Bioelectromagnetics.

[11]  K. Schoenbach,et al.  Nanosecond pulsed electric field (nsPEF) effects on cells and tissues: apoptosis induction and tumor growth inhibition , 2001, PPPS-2001 Pulsed Power Plasma Science 2001. 28th IEEE International Conference on Plasma Science and 13th IEEE International Pulsed Power Conference. Digest of Papers (Cat. No.01CH37251).

[12]  W. J. Dower,et al.  High efficiency transformation of E. coli by high voltage electroporation , 1988, Nucleic Acids Res..

[13]  Kenneth R. Foster,et al.  Thermal and nonthermal mechanisms of interaction of radio-frequency energy with biological systems , 2000 .

[14]  U. Pliquett,et al.  Nanosecond pulsed electric fields cause melanomas to self-destruct , 2006, Conference Record of the 2006 Twenty-Seventh International Power Modulator Symposium.

[15]  P. A. Mason,et al.  Pulsed microwave induced bioeffects , 2000 .

[16]  B E Stuck,et al.  Comparative effects of extremely high power microwave pulses and a brief CW irradiation on pacemaker function in isolated frog heart slices. , 2000, Bioelectromagnetics.

[17]  E. Neumann,et al.  Permeability changes induced by electric impulses in vesicular membranes , 1972, The Journal of Membrane Biology.

[18]  U. Zimmermann,et al.  Dielectric breakdown of cell membranes , 1974, Biophysics of structure and mechanism.

[19]  Laura Marcu,et al.  Ultrashort pulsed electric fields induce membrane phospholipid translocation and caspase activation: differential sensitivities of Jurkat T lymphoblasts and rat glioma C6 cells , 2003 .

[20]  K. Foster.  Comment: "Microwave irradiation influences on the state of human cell nuclei" [Y.G. Shckorbatov Et al., 19:414-419 (1998)]. , 2000, Bioelectromagnetics.

[21]  Microwave irradiation influences on the state of human cell nuclei. , 1998, Bioelectromagnetics.

[22]  G. A. Hofmann,et al.  Medical applications of electroporation , 2000 .