Laminar and cellular distribution of AMPA, kainate, and NMDA receptor subunits in monkey sensory–motor cortex

In situ hybridization histochemistry and immunocytochemistry were used to examine lamina‐ and cell‐specific expression of glutamate receptor (GluR) mRNAs and polypeptide subunits in motor and somatosensory cortex of macaque monkeys. Radioactive complementary RNA (cRNA) probes were prepared from cDNAs specific for α‐amino‐3‐hydroxy‐5‐methyl‐isoxozolepropionate (AMPA)/kainate (GluR1–GluR4), kainate (GluR5–GluR7), and N‐methyl‐D‐aspartate (NMDA; NR1, NR2A–NR2D) receptor subunits. AMPA/kainate and NR1, NR2A, and NR2B receptor transcripts show higher expression than other transcripts. All transcripts show lamina‐specific patterns of distribution. GluR2 and GluR4 mRNAs show higher expression than do GluR1 and GluR3 mRNAs. GluR6 transcript expression is higher than that of GluR5 and GluR7. NR1 mRNA expression is much higher than that of NR2 mRNAs. NR2C subunit expression is very low except for a very distinct band of high expression in layer IV of area 3b.

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