Characterization of the heterogeneous binding site affinity distributions in molecularly imprinted polymers.

Molecularly imprinted polymers (MIPs) are polymers that can be tailored with affinity and selectivity for a molecule of interest. Offsetting the low cost and ease of preparation of MIPs is the presence of binding sites that vary widely in affinity and selectivity. Presented is a review of methods that take into account binding site heterogeneity when calculating the binding properties of MIPs. These include the bi-Langmuir, Freundlich, and Langmuir-Freundlich binding models. These methods yield a measure of heterogeneity in the form of binding site affinity distributions and the heterogeneity index. Recent developments have made these methods surprisingly easy to use while also yielding more accurate measures of the binding properties of MIPs. These have allowed for easier comparison and optimization of MIPs. Heterogeneous binding models have also led to a better understanding of the imprinting process and of the advantages and limitations of MIPs in chromatographic and sensor applications.

[1]  Klaus Mosbach,et al.  Drug assay using antibody mimics made by molecular imprinting , 1993, Nature.

[2]  M. Borkovec,et al.  Regularized least-squares methods for the calculation of discrete and continuous affinity distributions for heterogeneous sorbents. , 1995, Environmental science & technology.

[3]  R. Sips,et al.  On the Structure of a Catalyst Surface , 1948 .

[4]  Wen‐Chien Lee,et al.  Chromatographic characteristics of cholesterol-imprinted polymers prepared by covalent and non-covalent imprinting methods. , 2002, Journal of chromatography. A.

[5]  Börje Sellergren,et al.  Molecularly imprinted polymers : man-made mimics of antibodies and their applications in analytical chemistry , 2001 .

[6]  G. Wulff,et al.  Stoichiometric noncovalent interaction in molecular imprinting , 2001, Bioseparation.

[7]  G. Wulff.  Molecular Imprinting in Cross‐Linked Materials with the Aid of Molecular Templates— A Way towards Artificial Antibodies , 1995 .

[8]  G. Wulff,et al.  Enzyme‐analogue built polymers, 4. On the synthesis of polymers containing chiral cavities and their use for the resolution of racemates , 1977 .

[9]  D. Spivak,et al.  Systematic study of steric and spatial contributions to molecular recognition by non-covalent imprinted polymers. , 2001, The Analyst.

[10]  G. Wulff,et al.  Enzyme-analogue built polymers, 5. On the specificity distribution of chiral cavities prepared in synthetic polymers† , 1977 .

[11]  B. Sellergren,et al.  Influence of Thermal Annealing on the Thermodynamic and Mass-Transfer Kinetic Properties of d- and l-Phenylalanine Anilide on Imprinted Polymeric Stationary Phases. , 1999, Analytical chemistry.

[12]  Toshifumi Takeuchi,et al.  A molecularly imprinted synthetic polymer receptor selective for atrazine , 1995 .

[13]  D. Rodbard,et al.  Characterization of ligand-binding systems by continuous affinity distributions of arbitrary shape. , 1980, Analytical biochemistry.

[14]  Mizuo Maeda,et al.  Molecular and ionic recognition with imprinted polymers , 1998 .

[15]  R. J. Umpleby,et al.  Measurement of the continuous distribution of binding sites in molecularly imprinted polymers , 2000 .

[16]  R. N. Shah,et al.  Characterization of molecularly imprinted polymers with the Langmuir-Freundlich isotherm. , 2001, Analytical chemistry.

[17]  Sarah C. Baxter,et al.  Application of the Freundlich adsorption isotherm in the characterization of molecularly imprinted polymers , 2001 .

[18]  K. Mosbach,et al.  Binding studies on substrate- and enantio-selective molecularly imprinted polymers , 1991 .

[19]  Kenneth J. Shea,et al.  Polymer complements to nucleotide bases. Selective binding of adenine derivatives to imprinted polymers , 1993 .

[20]  A. M. Puziy,et al.  Use of CONTIN for Calculation of Adsorption Energy Distribution , 1999 .

[21]  Börje Sellergren,et al.  Origin of peak asymmetry and the effect of temperature on solute retention in enantiomer separations on imprinted chiral stationary phases , 1995 .