ANTIBODY RESPONSE IN PATIENTS WITH PSEUDOMONAS AERUGINOSA INFECTION TO A ‘COMMON ANTIGEN’ FROM P. AERUGINOSA ANALYSED BY MEANS OF QUANTITATIVE IMMUNOELECTROPHORETIC METHODS

381 sera from 101 patients suffering from Pseudomonas aeruginosa infection have been investigated for antibodies against a ‘common antigen’ (CA) and other antigens from P. aeruginosa by means of crossed immunoelectrophoresis with intermediate gel, using a polyspecific P. aeruginosa antigen/antibody reference system. The earliest antibody response during the P. aeruginosa infections included increased CA antibodies in only 38% of the patients examined. The peak level of the antibody response included increased CA antibodies in 71% of the patients. By means of ‘absorption of antibodies in situ’, it was found that the antibody response to CA was directed against both the common cross‐reactive part of the CA molecule and the Pseudomonas‐specific part, with individual variations. During infections caused by other bacteria containing CA, one‐third of the patients developed antibodies against CA. The diagnostic implications of the results are discussed.

[1]  N. Høiby,et al.  AN ANTIGEN COMMON TO A WIDE RANGE OF BACTERIA , 1980, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology.

[2]  N. HØiby,et al.  Cross‐Reactions Between Haemophilus Influenzae and Nineteen Other Bacterial Species , 1979, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology.

[3]  N. HØiby,et al.  CROSS‐REACTIONS BETWEEN NEISSERIA MENINGITIDIS AND TWENTY‐SEVEN OTHER BACTERIAL SPECIES , 1978, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology.

[4]  L. Jacobsen,et al.  Pseudomonas aeruginosa infection in cystic fibrosis. Diagnostic and prognostic significance of Pseudomonas aeruginosa precipitins determined by means of crossed immunoelectrophoresis. , 1977, Scandinavian journal of respiratory diseases.

[5]  N. Høiby.  Pseudomonas aeruginosa infection in cystic fibrosis. Diagnostic and prognostic significance of pseudomonas aeruginosa precipitins determined by means of crossed immunoelectrophoresis. A survey. , 1977, Acta pathologica et microbiologica Scandinavica. Supplement.

[6]  N. Høiby,et al.  Cross-reactions between Bordetella pertussis and twenty-eight other bacterial species. , 2009, Acta pathologica et microbiologica Scandinavica. Section B, Microbiology.

[7]  N. Høiby.  Cross‐Reactions between Pseudomonas aeruginosa and Thirty‐Six Other Bacterial Species , 1975 .

[8]  N. Høiby.  Normally Occurring Precipitating Antibodies against Pseudomonas aeruginosa , 1975 .

[9]  B. Kaijser.  Immunological studies of an antigen common to many gram-negative bacteria with special reference to E. coli. Characterization and biological significance. , 1975, International archives of allergy and applied immunology.

[10]  N. Høiby,et al.  Identification and quantitation of precipitins against Pseudomonas aeruginosa in patients with cystic fibrosis by means of crossed immunoelectrophoresis with intermediate gel. , 2009, Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology.