Rapid determination of paeoniflorin from Paeonia sinjiang K. Y. Pan. by rapid resolution liquid chromatography

A rapid, effective, binary reverse phase rapid resolution liquid chromatographic method has been developed for the determination of Paeoniflorin extracted from Paeonia sinjiang K. Y. Pan. with short run time. RRLC separation was achieved by using Agilent (Zorbas XDB-C18 4.6 mm × 50 mm, 1.8 μm) column with mobile phase complosed of methanol and 0.05 mol/l potassium phosphate monobasic. Flow rate was at 1.0 ml/min. Retention time was at about 1.16 min. Recovery rate was 99.8% and relative standard deviation of three replicate samples tested was 1.8%. RRLC method has been applied to both in vitro studies of paeoniflorin formulation and drug estimation in biological samples.

[1]  G. Chou,et al.  A new phenolic glycoside from the roots of Paeonia veitchii , 2006, Journal of Asian natural products research.

[2]  Xiao-chang Ma,et al.  [Preliminary study on change of serum proteome in noxious heat blood stasis syndrome treated by radix Paeoniae rubra]. , 2005, Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine.

[3]  Zheng-Tao Wang,et al.  [Quality evaluation of prepared slices of Paeonia lactiflon--determination of paeoniflorin by HPLC]. , 2004, Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica.

[4]  J. Wang,et al.  [Study on activating blood and eliminating stasis of total paeony glycoside(TPG)]. , 2000, Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials.

[5]  Wang Yu-qing Determination of Paeoniflorin in Radix Paeonia hybrida from Different Habitats , 2008 .

[6]  Wu Qiao-feng Determination of Peoniflorin and Benzoic Acid in Duration of Cultivation of Plant in Radix Paeoniae Rubra , 2008 .