Isolation of motile spermatozoa from semen samples using microfluidics.

[1]  G. Whitesides,et al.  Soft lithography in biology and biochemistry. , 2001, Annual review of biomedical engineering.

[2]  D. Liu,et al.  Comparison of Percoll, mini-Percoll and swim-up methods for sperm preparation from abnormal semen samples. , 1992, Human reproduction.

[3]  A Zini,et al.  Influence of semen processing technique on human sperm DNA integrity. , 2000, Urology.

[4]  R. Ericsson Sex selection via albumin columns: 20 years of results. , 1994, Human reproduction.

[5]  Shuichi Takayama,et al.  Passively driven integrated microfluidic system for separation of motile sperm. , 2003, Analytical chemistry.

[6]  S. Pang,et al.  Effects of pentoxifylline on sperm motility and hyperactivation in normozoospermic and normokinetic semen. , 1993, Fertility and sterility.

[7]  Y. Englert,et al.  Comparative auto-controlled study between swim-up and Percoll preparation of fresh semen samples for in-vitro fertilization. , 1991, Human reproduction.

[8]  George M. Whitesides,et al.  Laminar flows: Subcellular positioning of small molecules , 2001, Nature.

[9]  P. Yager,et al.  Diffusion-based extraction in a microfabricated device , 1997 .

[10]  D. Mortimer,et al.  Sperm preparation techniques and iatrogenic failures of in-vitro fertilization. , 1991, Human reproduction.

[11]  L. Scott,et al.  Use of postseparation sperm parameters to determine the method of choice for sperm preparation for assisted reproductive technology. , 1995, Fertility and sterility.

[12]  J. C. Andrews,et al.  A rapid sperm motility bioassay procedure for quality-control testing of water and culture media , 1988, Journal of in Vitro Fertilization and Embryo Transfer.

[13]  R. Aitken,et al.  Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques. , 1988, Journal of andrology.

[14]  P. Yager,et al.  Microfluidic Diffusion-Based Separation and Detection , 1999, Science.

[15]  G. Seidel,et al.  Sexing mammalian sperm--overview. , 1999, Theriogenology.

[16]  B. T. Storey,et al.  Centrifugation of human spermatozoa induces sublethal damage; separation of human spermatozoa from seminal plasma by a dextran swim-up procedure without centrifugation extends their motile lifetime. , 1993, Human reproduction.

[17]  J. Antoine,et al.  The management of multiple pregnancies after induction for superovulation. , 1988, Human reproduction.

[18]  J. D. Neill,et al.  The Physiology of reproduction , 1988 .

[19]  D. Beebe,et al.  Microfluidic technology for assisted reproduction. , 2002, Theriogenology.

[20]  D. Sklan,et al.  Effect of caffeine on increasing the motility of frozen human sperm. , 1977, Fertility and sterility.

[21]  G. Whitesides,et al.  Microfabrication inside capillaries using multiphase laminar flow patterning , 1999, Science.

[22]  I. Schiff,et al.  Preparation by differential gradient centrifugation is better than swim-up in selecting sperm with normal morphology (strict criteria). , 1998, Fertility and sterility.

[23]  B. Bavister,et al.  Deterioration of stored culture media as monitored by a sperm motility bioassay , 1988, Journal of in Vitro Fertilization and Embryo Transfer.

[24]  L. Geerts,et al.  Sperm morphology and IVF pregnancy rate: comparison between Percoll gradient centrifugation and swim-up procedures. , 1991, Human reproduction.

[25]  G. Whitesides,et al.  Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). , 1998, Analytical chemistry.

[26]  B. Bavister,et al.  Stimulation of rhesus monkey sperm capacitation by cyclic nucleotide mediators. , 1984, Journal of reproduction and fertility.

[27]  E. Nieschlag,et al.  Improvement in quality of cryopreserved human spermatozoa by swim-up before freezing. , 1994, International journal of andrology.

[28]  D. Katz,et al.  Differences in the movement of morphologically normal and abnormal human seminal spermatozoa. , 1982, Biology of reproduction.

[29]  M. Mandrioli,et al.  The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies. , 2000, Human reproduction.