The biochemical mechanism of selective heat sensitivity of cancer cells. I. Studies on cellular respiration.

Resume On a etudie l'effet de la temperature, entre 38 et 43° C, sur l'incorporation des precurseurs marques dans le DNA, RNA et les proteines des cellules de l'hepatome de Novikoff et de l'hepatome 5123 de Morris. La temperature a provoque une inhibition marquee; celle-ci a ete observee dans les deux types de cellules cancereuses, alors que les cellules de foie regenerant ne sont pas affectees. Cet effet de la temperature a ete observe aussi dans les cellules d'un osteosarcome humain. Les preparations acellulaires sont insensibles a la temperature. Le d-glucose, ajoute au melange d'incubation, cause une inhibition tres evidente. La filipine et l'ethanol inhibent l'incorporation dans les cellules cancereuses a des concentrations qui n'ont pas d'effet appreciable sur les cellules du foie regenerant. Pour expliquer le mecanisme d'action de la chaleur sur les cellules cancereuses, on peut envisager des alterations des membranes.

[1]  L. Morasca,et al.  Effects of temperature on the kinetics of the mitotic cycle of mammalian cells in culture. , 1965, Experimental cell research.

[2]  D. T. Poole.  Intracellular pH of the Ehrlich ascites tumour cell as it is affected by sugars and sugar derivatives. , 1967, The Journal of biological chemistry.

[3]  J. Lloyd,et al.  Lysosomal Enzyme Inhibition by Trypan Blue: A Theory of Teratogenesis , 1967, Science.

[4]  P N RAO,et al.  HeLa Cells: Effects of Temperature on the Life Cycle , 1965, Science.

[5]  J. Goa.  A micro biuret method for protein determination; determination of total protein in cerebrospinal fluid. , 1953, Scandinavian journal of clinical and laboratory investigation.

[6]  B. Christophersen.  EFFECTS OF ETHANOL ON MITOCHONDRIAL OXIDATIONS. , 1964, Biochimica et biophysica acta.

[7]  S. Kinsky,et al.  Interaction of filipin and derivatives with erythrocyte membranes and lipid dispersions: electron microscopic observations. , 1967, Biochimica et biophysica acta.

[8]  O. Touster,et al.  Biochemical characterization of the lysosomes of ehrlich ascites tumor cells , 1967 .

[9]  C. Heidelberger,et al.  In vitro synthesis of acidsoluble thymine compounds by human neoplastic tissues. , 1963, Cancer research.

[10]  N. Westermark.  The Effect of Heat upon Rat-Tumors1 , 1927 .

[11]  William H. Summerson,et al.  The colorimetric determination of lactic acid in biological material. , 1941 .

[12]  G. Weissmann,et al.  Effect of polyene antibiotics of phospholipid spherules containing varying amounts of charged components. , 1967, Biochimica et biophysica acta.

[13]  A. Allison,et al.  Chromosome Damage in Human Diploid Cells following Activation of Lysosomal Enzymes , 1965, Nature.

[14]  R. Wattiaux,et al.  Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. , 1955, The Biochemical journal.

[15]  P. Plagemann.  Phenethyl alcohol: reversible inhibition of synthesis of macromolecules and disaggregation of polysomes in rat hepatoma cells. , 1968, Biochimica et biophysica acta.

[16]  H. Kalant,et al.  Effects of lower alcohols on potassium transport and microsomal adenosine-triphosphatase activity of rat cerebral cortex. , 1966, The Biochemical journal.

[17]  W. C. Schneider,et al.  Intracellular distribution of enzymes; the oxidation of octanoic acid by rat liver fractions. , 1948, The Journal of biological chemistry.

[18]  R. Porter,et al.  The isolation and properties of a proteolytic enzyme, cathepsin D, from bovine spleen. , 1960, The Biochemical journal.

[19]  W. C. Schneider,et al.  Phosphorus compounds in animal tissues; extraction and estimation of desoxypentose nucleic acid and of pentose nucleic acid. , 1945, The Journal of biological chemistry.

[20]  C. Heidelberger,et al.  Selective heat sensitivity of cancer cells. Biochemical and clinical studies , 1967 .

[21]  M. Hoagland,et al.  Inhibitors of protein synthesis in rat liver microsome fractions. , 1967, The Journal of biological chemistry.

[22]  S. Kinsky,et al.  Further studies on the hemolytic action of filipin and derivatives. , 1967, Biochimica et biophysica acta.

[23]  W. Wunner,et al.  Use of a single tissue extract to determine cellular protein and nucleic acid concentrations and rate of amino acid incorporation. , 1965, Analytical biochemistry.

[24]  G. Weissmann,et al.  The action of polyene antibiotics on phospholipid-cholesterol structures. , 1967, The Journal of biological chemistry.

[25]  G. Rotilio,et al.  The biochemical mechanism of selective heat sensitivity of cancer cells. II. Studies on nucleic acids and protein synthesis. , 1969, European journal of cancer.

[26]  G. Weissmann,et al.  Studies of lysosomes. 8. The effect of polyene antibiotics on lysosomes. , 1967, Biochemical pharmacology.

[27]  C. J. Bos,et al.  Studies on plasma membranes. VI. Differences in the effect of temperature on the ATPase and (Na+-K+)-ATPase activities of plasma membranes isolated from rat liver and hepatoma. , 1968, Biochimica et biophysica acta.

[28]  H. Lardy,et al.  Oxidative phosphorylations; rôle of inorganic phosphate and acceptor systems in control of metabolic rates. , 1952, The Journal of biological chemistry.

[29]  H. Munro,et al.  The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. , 1962, Biochimica et biophysica acta.

[30]  Y. Israel,et al.  Effect of Ethanol on the Transport of Sodium in Frog Skin , 1963, Nature.

[31]  W. A. Green.  Aspects of some co-factor requirements in tumour metabolism , 1966 .

[32]  C. Heidelberger,et al.  Intracellular distribution of radioactivity in nucleic acid nucleotides and proteins following simultaneous administration of P32 and glycine-2-C14. , 1953, Cancer research.

[33]  J. Tennant.  EVALUATION OF THE TRYPAN BLUE TECHNIQUE FOR DETERMINATION OF CELL VIABILITY , 1964, Transplantation.

[34]  H. Inoue,et al.  THE BIOCHEMISTRY OF ANIMAL CELLS. I. THE EFFECT OF CORTICOTEROIDS ON LEAKAGE OF ENZYMES FROM DISPERSED RAT LIVER CELLS. , 1964, The Journal of biological chemistry.

[35]  H. Lardy,et al.  Cellular oxidative phosphorylation. 3. Measurement in chemically modified cells. , 1967, Biochemistry.

[36]  D. Maclennan,et al.  Studies on the electron transfer system. LXVI. Effect of diphosphopyridine nucleotide deficiency on respiration, respiratory control, and phosphorylation in mitochondria. , 1966, The Journal of biological chemistry.