CATHEPSIN B, CATHEPSIN C AND ARYLAMIDASE IN RABBIT CORNEA

The 3 peptide hydrolases cathepsin B, cathepsin C and arylamidase have been assayed in rabbit cornea with the use of fluorigenic derivatives of β‐naphthylamine. The optimal reaction conditions and kinetic properties are described. The assay procedures which are simple and very sensitive can be used for studying the release of these enzymes from corneal cells, and further their eventual role in the pathological events following the influence of external agents.

[1]  G. Volden,et al.  CHARACTERIZATION AND QUANTIFICATION OF ACID PHOSPHATASE AND GLYCOSIDE HYDROLASES IN RABBIT CORNEA , 1982, Acta ophthalmologica.

[2]  P. Thune,et al.  Acid hydrolases in blister fluid , 1979, The British journal of dermatology.

[3]  G. Volden Acid hydrolases in blister fluid , 1978, The British journal of dermatology.

[4]  A. Barrett,et al.  Lysis and killing of bacteria by lysosomal proteinases , 1976, Infection and immunity.

[5]  E. Davidson,et al.  Fractionation of the rat liver enzymes that hydrolyze benzoyl-arginine-2-naphthylamide. , 1975, Biochimica et biophysica acta.

[6]  K. Otto,et al.  Improved purification of cathepsin B1 and cathepsin B2. , 1975, Biochimica et biophysica acta.

[7]  A. Barrett,et al.  Cathepsin B1. A lysosomal enzyme that degrades native collagen. , 1974, The Biochemical journal.

[8]  Portland Press Ltd Human cathepsin B1. Purification and some properties of the enzyme , 1973 .

[9]  J. Dingle,et al.  Cathepsins BI and D. Action on human cartilage proteoglycans. , 1973, Biochimica et biophysica acta.

[10]  A. Barrett Human cathepsin B1. Purification and some properties of the enzyme. , 1973, The Biochemical journal.

[11]  A. Tappel,et al.  Cathepsins D, A and B, and the effect of pH in the pathway of protein hydrolysis. , 1971, Biochimica et biophysica acta.

[12]  C. Dohlman,et al.  Peptidase in the cornea. , 1969, Experimental eye research.

[13]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[14]  Y. Aoki,et al.  LYSOSOMAL ENZYMES , 2017 .

[15]  K. Otto [On a new cathepsin. Purification from bovine spleen, properties, as well as comparison with cathepsin B]. , 1967, Hoppe-Seyler's Zeitschrift fur physiologische Chemie.

[16]  J. Bouma,et al.  Intracellular distribution of cathepsin B and cathepsin C in rat liver. , 1966, Biochimica et biophysica acta.