Growth enhancement of transgenic mice expressing human insulin-like growth factor I.

A line of transgenic mice carrying a chimeric gene composed of human insulin-like growth factor I (IGF-I) coding sequences fused to the mouse metallothionein I promoter was generated to study the effects of chronically elevated exposure to IGF-I. Mice in this line overexpress IGF-I in most tissues studied and have circulating IGF-I levels 1.5 times the normal value. This results in a growth response manifested by a 1.3-fold increase in weight as a result of selective organomegaly without an apparent increase in skeletal growth. In addition, expression of the endogenous GH and IGF-I genes is inhibited. These results are consistent with IGF-I playing an important role in the control of somatic growth.

[1]  R. Palmiter,et al.  Expression of insulin-like growth factor I in transgenic mice with elevated levels of growth hormone is correlated with growth. , 1988, Endocrinology.

[2]  E. Froesch,et al.  Recombinant human insulin-like growth factor I stimulates growth and has distinct effects on organ size in hypophysectomized rats. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[3]  L. Mathews,et al.  Regulation of insulin-like growth factor messenger ribonucleic acid in rat growth plate by growth hormone. , 1988, Endocrinology.

[4]  K. Hall,et al.  The Effects of Biosynthetic Insulin-Like Growth Factor-1 Supplementation on Somatic Growth, Maturation, and Erythropoiesis on the Neonatal Rat , 1988, Pediatric Research.

[5]  R. Palmiter,et al.  Introns increase transcriptional efficiency in transgenic mice. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[6]  R. Steiner,et al.  The effect of hypophysectomy and growth hormone administration on pre-prosomatostatin messenger ribonucleic acid in the periventricular nucleus of the rat hypothalamus. , 1988, Endocrinology.

[7]  A. Lindahl,et al.  Mechanism of the stimulatory effect of growth hormone on longitudinal bone growth. , 1987, Endocrine reviews.

[8]  A. Lindahl,et al.  Differential effects of growth hormone and insulin-like growth factor I on colony formation of epiphyseal chondrocytes in suspension culture in rats of different ages. , 1987, Endocrinology.

[9]  R. Steiner,et al.  Reduced preprosomatostatin messenger ribonucleic acid in the periventricular nucleus of hypophysectomized rats determined by quantitative in situ hybridization. , 1987, Endocrinology.

[10]  A. Hoffman,et al.  The growth hormone (GH)-releasing hormone (GHRH)-GH-somatomedin axis: evidence for rapid inhibition of GHRH-elicited GH release by insulin-like growth factors I and II. , 1987, Endocrinology.

[11]  V. Han,et al.  Characterization of somatomedin/insulin-like growth factor receptors and correlation with biologic action in cultured neonatal rat astroglial cells , 1987, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[12]  L. Underwood,et al.  [21] Estimation of tissue concentrations of somatomedin C/insulin-like growth factor I , 1987 .

[13]  R. Clark,et al.  Recombinant human insulin-like growth factor: testing the somatomedin hypothesis in hypophysectomized rats. , 1987, The Journal of endocrinology.

[14]  R. Palmiter,et al.  Regulation of insulin-like growth factor I gene expression by growth hormone. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[15]  S. M. Russell,et al.  Evidence suggesting that the direct growth-promoting effect of growth hormone on cartilage in vivo is mediated by local production of somatomedin. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[16]  J. L. Brande,et al.  Biosynthetic Somatomedin C (SM-C/IGF-I) Increases the Length and Weight of Snell Dwarf Mice , 1986, Pediatric Research.

[17]  A. Dahlström,et al.  Regulation by growth hormone of number of chondrocytes containing IGF-I in rat growth plate. , 1986, Science.

[18]  D. Porte,et al.  Identification of binding sites for an insulin-like growth factor (IGF-I) in the median eminence of the rat brain by quantitative autoradiography. , 1986, Endocrinology.

[19]  R. Horikawa,et al.  Insulin-like growth factor I stimulates growth in normal growing rats. , 1986, European journal of pharmacology.

[20]  A. Lindahl,et al.  Growth hormone potentiates colony formation of epiphyseal chondrocytes in suspension culture. , 1986, Endocrinology.

[21]  D. K. Didier,et al.  Organization and sequence of the human insulin-like growth factor I gene. Alternative RNA processing produces two insulin-like growth factor I precursor peptides. , 1986, The Journal of biological chemistry.

[22]  S. Melmed,et al.  Autoregulation of pituitary growth hormone messenger ribonucleic acid levels in rats bearing transplantable mammosomatotrophic pituitary tumors. , 1986, Endocrinology.

[23]  S. Melmed,et al.  Insulin-like growth factor I action on rat anterior pituitary cells: suppression of growth hormone secretion and messenger ribonucleic acid levels. , 1986, Endocrinology.

[24]  P. Rotwein,et al.  Two insulin-like growth factor I messenger RNAs are expressed in human liver. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[25]  D. Gerhard,et al.  Isolation of the human insulin-like growth factor genes: insulin-like growth factor II and insulin genes are contiguous. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[26]  R. Palmiter,et al.  Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[27]  E. Spencer,et al.  Local injections of human or rat growth hormone or of purified human somatomedin-C stimulate unilateral tibial epiphyseal growth in hypophysectomized rats. , 1985, Endocrinology.

[28]  R. Palmiter,et al.  Specific expression of an elastase–human growth hormone fusion gene in pancreatic acinar cells of transgenic mice , 1985, Nature.

[29]  R. Palmiter,et al.  Use of gene transfer to increase animal growth. , 1985, Cold Spring Harbor symposia on quantitative biology.

[30]  A. Stiles,et al.  Tissue concentrations of somatomedin C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[31]  G. Bell,et al.  Sequence of a cDNA clone encoding human preproinsulin-like growth factor II , 1984, Nature.

[32]  R. Palmiter,et al.  Metallothionein-human GH fusion genes stimulate growth of mice. , 1983, Science.

[33]  R. Palmiter,et al.  A practical approach for quantitating specific mRNAs by solution hybridization. , 1983, Analytical biochemistry.

[34]  B. Posner,et al.  Insulin-like growth factors: a role in growth hormone negative feedback and body weight regulation via brain. , 1983, Science.

[35]  J. Jansson,et al.  Sites of action of testosterone and estradiol on longitudinal bone growth. , 1983, The American journal of physiology.

[36]  S. van Buul-Offers,et al.  The influence of growth hormone, somatomedins, prolactin and thyroxine on the morphology of the proximal tibial epiphysis and growth plate of Snell dwarf mice. , 1983, Growth.

[37]  Michael G. Rosenfeld,et al.  Dramatic growth of mice that develop from eggs microinjected with metallothionein–growth hormone fusion genes , 1982, Nature.

[38]  W. Jelkmann,et al.  A new candidate for the regulation of erythropoiesis , 1982, FEBS letters.

[39]  W. Vale,et al.  Characterization of a growth hormone-releasing factor from a human pancreatic islet tumour , 1982, Nature.

[40]  J. Jansson,et al.  Growth hormone stimulates longitudinal bone growth directly. , 1982, Science.

[41]  E. Schoenle,et al.  Insulin-like growth factor I stimulates growth in hypophysectomized rats , 1982, Nature.

[42]  R L Hintz,et al.  Somatomedin-C mediates growth hormone negative feedback by effects on both the hypothalamus and the pituitary. , 1981, Science.

[43]  N. Ling,et al.  Hypothalamic Polypeptide That Inhibits the Secretion of Immunoreactive Pituitary Growth Hormone , 1973, Science.

[44]  W. Daughaday,et al.  Somatomedin: Proposed Designation for Sulphation Factor , 1972, Nature.

[45]  J. Tanner,et al.  A longitudinal study of the growth of the black-hooded rat: methods of measurement and rates of growth for skull, limbs, pelvis, nose-rump and tail lengths. , 1970, Journal of anatomy.