Chip‐Based Cell Electrofusion

Cell fusion is a rapid developing cytobiological method utilizing engineering approaches to fuse two or more cells into one hybrid. The hybrid cell can be further cultivated into a new species or a cell-engineering product. After many years of development, cell fusion is becoming a powerful tool for biological, medical and agricultural research. At present, electrofusion is widely used to achieve cell fusion due to its high efficiency and maneuverability. On-chip cell electrofusion is receiving more and more attention as a result of its superior performance. This review will discuss the basic theory of chip-based cell electrofusion, the pairing method, low- and high-throughput cell electrofusion, and the possible ways to overcome the major limitations in existing electrofusion methods.

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