Synthesis, characterization, and anticancer properties of rare earth complexes with Schiff base and o-phenanthroline

Abstract Five novel ternary complexes of the rare earth ions with o-phenanthroline(Phen) and Schiff base salicylaldehyde L-phenylalanine(KHL) were synthesized in ethanol. Their compositions were characterized by elemental analysis, molar conductance, 1 H NMR, FT-IR, and Raman spectra. The formulas of the complexes were verified to be RE(L)(Phen)Cl(H 2 O) (RE=La 3+ , Ce 3+ , Nd 3+ , Er 3+ , and Gd 3+ ; L=Schiff base salicylaldehyde L-phenylalanine; phen=o-phenanthroline). Methyl thiazolyl tetrazolium (MTT) colorimetry and flow cytometry were used to test the anticancer effect of the complexes with K562 tumor cell. The research showed that the complexes could inhibit K562 tumor cell¢s growth, generation, and induce apoptosis. The inhibition ratio was accelerated by increasing the dosage, and it had significant positive correlation with the medication dosage.

[1]  N. Fox,et al.  Chemosensitivity testing of fresh leukaemia cells using the MTT colorimetric assay , 1989, British journal of haematology.

[2]  J. Hayashi,et al.  Rapid uptake of tyrphostin into A431 human epidermoid cells is followed by delayed inhibition of epidermal growth factor (EGF)-stimulated EGF receptor tyrosine kinase activity , 1991, Molecular and cellular biology.

[3]  C. Faaland,et al.  Inhibition of epidermal growth factor-stimulated EGF receptor tyrosine kinase activity in A431 human epidermoid carcinoma cells by polyamines. , 1995, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.

[4]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[5]  K. Nakamoto.  Infrared and Raman Spectra of Inorganic and Coordination Compounds , 1978 .

[6]  Xia Wu,et al.  Progress of Spectral Probes for Nucleic Acids , 2003 .

[7]  B. Yan,et al.  A novel quaternary dinuclear luminescent terbium complex Tb2(phth)2(Hphth)2(phen)2(H2O)4: hydrothermal synthesis, crystal structure and photophysics , 2004 .

[8]  Hong Li,et al.  Synthesis, crystal structure and DNA cleavage activities of copper(II) complexes with asymmetric tridentate ligands. , 2004, Journal of inorganic biochemistry.

[9]  J. Richter,et al.  A cDNA clone for a polyadenylated RNA-binding protein of Xenopus laevis oocytes hybridizes to four developmentally regulated mRNAs , 1985, Molecular and cellular biology.

[10]  W. Horrocks,et al.  Lanthanide ions as redox probes of long-range electron transfer in proteins , 1997 .

[11]  T. Thomas,et al.  Regulation of cyclin B1 by estradiol and polyamines in MCF-7 breast cancer cells. , 1994, Cancer research.

[12]  W. Geary.  The use of conductivity measurements in organic solvents for the characterisation of coordination compounds , 1971 .

[13]  M. Devereux,et al.  Synthesis, X-ray crystal structures and biomimetic and anticancer activities of novel copper(II)benzoate complexes incorporating 2-(4'-thiazolyl)benzimidazole (thiabendazole), 2-(2-pyridyl)benzimidazole and 1,10-phenanthroline as chelating nitrogen donor ligands. , 2007, Journal of inorganic biochemistry.