Serological detection of variable region (Vh) subgroups of Ig heavy chains

Serological test systems were established for determining the heavy- chain variable region (Vh) subgroups of immunoglobulin heavy chains. Myeloma proteins with known Vh subgroups based on amino acid sequence were utilized as the primary basis of reference for analysis by hemagglutination and hemagglutination inhibition. Good agreement between the chemical and serological typing was obtained and nonoverlapping systems established for the three major Vh subgroups. In a survey of 167 myeloma proteins, all except two were exclusively positive in one of the three systems. The two exceptions may represent a fourth subgroup. There was an overall incidence ratio of 1:2:3 for VhI:VhII:VhIII subgroups. Some differences in the overall ratios were encountered within the immunoglobulin classes. Certain advantages of the serological typing antisera were discussed with special emphasis on their use for studies of Vh antigens on the membranes of lymphocytes.

[1]  Ø. Førre,et al.  Restriction of Human Immune Antibodies to Heavy‐Chain Variable Subgroups , 1976, Scandinavian journal of immunology.

[2]  C. Milstein,et al.  N‐terminal amino acid sequence of a human delta‐chain myeloma protein , 1976, European journal of immunology.

[3]  Ø. Førre,et al.  Antibody Activity of Heavy and Light Chains and Recombined IgG of Human IgG Anti‐D , 1976, Scandinavian journal of immunology.

[4]  A. Moulin,et al.  Chemical typing of human immunoglobulin VH subgroups. , 1975, Immunochemistry.

[5]  H. Binz,et al.  Idiotype‐Positive T Lymphocytess , 1975 .

[6]  J. D. Capra,et al.  Distribution and association of heavy and light chain variable region subgroups among human IgA immunoglobulins. , 1975, Journal of immunology.

[7]  J. Kehoe,et al.  Hypervariable regions, idiotypy, and the antibody-combining site. , 1975, Advances in immunology.

[8]  T. Kindt,et al.  Rabbit immunoglobulin allotypes: structure, immunology, and genetics. , 1975, Advances in immunology.

[9]  H. Binz,et al.  Idiotype-positive T lymphocytes. , 1975, Scandinavian journal of immunology.

[10]  A. Solomon,et al.  Immunochemical classification of nonisolated light chains of immunoglobulins. , 1974, Journal of immunology.

[11]  J. D. Capra,et al.  Structure of antibodies with shared idiotypy: the complete sequence of the heavy chain variable regions of two immunoglobulin M anti-gamma globulins. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[12]  F. Putnam,et al.  The switch point in mu heavy chains of human IgM immunoglobulins. , 1974, Biochemistry.

[13]  K. Sletten,et al.  Immunochemical Detection of the VKIV Subgroup , 1974, Scandinavian journal of immunology.

[14]  J. Kehoe,et al.  Variable region sequences of five human immunoglobulin heavy chains of the VH3 subgroup: definitive identification of four heavy chain hypervariable regions. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[15]  R. Winchester,et al.  SIMILARITIES IN THE LIGHT CHAINS OF ANTI-γ-GLOBULINS SHOWING CROSS-IDIOTYPIC SPECIFICITIES , 1974, The Journal of experimental medicine.

[16]  A. Shimizu,et al.  Complete Amino Acid Sequence of the Mu Heavy Chain of a Human IgM Immunoglobulin , 1973, Science.

[17]  J. Gally.  Structure of Immunoglobulins , 1973 .

[18]  H. Kunkel,et al.  Human immunoglobulins: classes, subclasses, genetic variants, and idiotypes. , 1973, Advances in immunology.

[19]  J. Natvig,et al.  Three New Fragments, F(ab)2, F(c)2, and Fab/c, Obtained by Papain Proteolysis of Normal Human IgG. , 1972, Scandinavian journal of immunology.

[20]  R. Williams,et al.  Light chain sequences of human IgM cold agglutinins (variable-region subgroups amino-acid sequence-kappa light chain-N-terminal). , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[21]  H. P. Treffers.  The Genetic Markers of Human Immunoglobulins , 1971, The Yale Journal of Biology and Medicine.

[22]  M. Turner,et al.  Localization of Gm markers to different molecular regions of the Fc fragment. , 1971, Clinical and experimental immunology.

[23]  E. Osserman,et al.  Subgroup-specific antigenic marker on immunoglobulin lambda chains: identification of three subtypes of the variable region. , 1970, Journal of immunology.

[24]  A. Shimizu,et al.  Three Variable-Gene Pools common to IgM, IgG and IgA Immunoglobulins , 1970, Nature.

[25]  N. Hogg,et al.  The amino acid sequences of the Fd fragments of two human gamma-1 heavy chains. , 1970, The Biochemical journal.

[26]  L. Rivat,et al.  [Genetic markers of human immunoglobulins]. , 1968, L' Annee biologique.

[27]  H. Fudenberg,et al.  Chromic chloride: a coupling reagent for passive hemagglutination reactions. , 1967, Journal of immunology.

[28]  H. Kunkel,et al.  Individual Antigenic Specificity of Isolated Antibodies , 1963, Science.

[29]  H. Kunkel,et al.  Zone Electrophoresis in a Starch Supporting Medium , 1952, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.