The polycistronic mRNA of the Zymomonas mobilis glf-zwf-edd-glk operon is subject to complex transcript processing

The full-length 6.14-kb polycistronic glf-zwf-edd-glk mRNA from Zymomonas mobilis appears to be processed by endonucleolytic cleavage, resulting in the formation of several discrete transcripts. Northern analysis and transcript mapping revealed that the processed transcripts correspond to functional mono-, di-, or tricistronic messages. The relative abundance of the gene-specific, functional messages was measured. Expression of zwf and edd correlated well with functional message levels. Disproportionally high levels of the glk-specific mRNAs might compensate for the instability of glucokinase by allowing increased translation. The relative abundance of the discrete transcripts was shown to be a function of their respective decay rates. Northern analysis of the fate of the 6.14-kb transcript after inhibition of transcription by rifampin showed that the abundance of shorter, more stable transcripts increased at the expense of longer, less stable transcripts. This is suggestive of endonucleolytic mRNA processing. The most abundant 5' and 3' transcript ends were found to lie within secondary structures that probably impart stability to the most abundant mRNAs.

[1]  J. Liu,et al.  The Zymomonas mobilis glf, zwf, edd, and glk genes form an operon: localization of the promoter and identification of a conserved sequence in the regulatory region , 1992, Journal of bacteriology.

[2]  J. Liu,et al.  Cloning, characterization and expression of the Zymononas mobilis eda gene that encodes 2‐keto‐3‐deoxy‐6‐phosphogluconate aldolase of the Entner‐Doudoroff pathway , 1991, Molecular microbiology.

[3]  R K Scopes,et al.  Gel electrophoretic analysis of Zymomonas mobilis glycolytic and fermentative enzymes: identification of alcohol dehydrogenase II as a stress protein , 1991, Journal of bacteriology.

[4]  J. Belasco,et al.  Structure and function of a bacterial mRNA stabilizer: analysis of the 5' untranslated region of ompA mRNA , 1991, Journal of bacteriology.

[5]  D. Kennell,et al.  RNase I*, a form of RNase I, and mRNA degradation in Escherichia coli , 1991, Journal of bacteriology.

[6]  L. Lindahl,et al.  Intermediates in the degradation of mRNA from the lactose operon of Escherichia coli. , 1991, Nucleic acids research.

[7]  T. Conway,et al.  Cloning, characterization, and nucleotide sequence analysis of a Zymomonas mobilis phosphoglucose isomerase gene that is subject to carbon source-dependent regulation , 1991, Journal of bacteriology.

[8]  G. Mackie Specific endonucleolytic cleavage of the mRNA for ribosomal protein S20 of Escherichia coli requires the product of the ams gene in vivo and in vitro , 1991, Journal of bacteriology.

[9]  L. Ingram,et al.  Segmental message stabilization as a mechanism for differential expression from the Zymomonas mobilis gap operon , 1991, Journal of bacteriology.

[10]  T. Conway,et al.  Sequence and genetic organization of a Zymomonas mobilis gene cluster that encodes several enzymes of glucose metabolism , 1990, Journal of bacteriology.

[11]  G. Mackie Stabilization of the 3' one-third of Escherichia coli ribosomal protein S20 mRNA in mutants lacking polynucleotide phosphorylase , 1989, Journal of bacteriology.

[12]  G. Brawerman mRNA decay: Finding the right targets , 1989, Cell.

[13]  D. Kennell,et al.  Evidence for endonucleolytic cleavages in decay of lacZ and lacI mRNAs , 1988, Journal of bacteriology.

[14]  Ö. Melefors,et al.  Site-specific endonucleolytic cleavages and the regulation of stability of E. coli ompA mRNA , 1988, Cell.

[15]  L. Viikari,et al.  CARBOHYDRATE METABOLISM IN ZYMOMONAS , 1988 .

[16]  L. Ingram,et al.  Glycolytic flux in Zymomonas mobilis: enzyme and metabolite levels during batch fermentation , 1987, Journal of bacteriology.

[17]  F. Neidhardt,et al.  Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology , 1987 .

[18]  D. Kennell CHAPTER 4 – The Instability of Messenger RNA in Bacteria , 1986 .

[19]  R. Scopes,et al.  Simultaneous purification and characterization of glucokinase, fructokinase and glucose-6-phosphate dehydrogenase from Zymomonas mobilis. , 1985, The Biochemical journal.

[20]  R. Scopes,et al.  Use of differential dye-ligand chromatography with affinity elution for enzyme purification: 6-phosphogluconate dehydratase from Zymomonas mobilis. , 1984, Analytical biochemistry.

[21]  J. Devereux,et al.  A comprehensive set of sequence analysis programs for the VAX , 1984, Nucleic Acids Res..

[22]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .