Magnetic manipulation and multimodal imaging for single cell direct mechanosensing

Wilson, Robert L. M.S.B.M.E., Purdue University, May 2015. Magnetic Manipulation and Multimodal Imaging for Single Cell Direct Mechanosensing. Major Professor: Corey P. Neu. The study of internal mechanics of single cells is paramount to understand mechanisms of mechanoregulation. External loading and cell-mediated force generation result in changes in cell shape, rheology, and the deformation of subcellular structures such as the nucleus. Moreover, alterations in the processes that regulate these responses have been further correlated to specific pathologies. Cellular deformation is often studied through application of forces in the environment of the cell, relying on strain and stress transfer through focal adhesions and the cytoskeletal system. However, the transfer of these external forces to internal mechanics can introduce uncertainties in the interpretation of subcellular responses. Our group has focused on minimally-invasive techniques for the study of internal mechanical perturbation and mechanobiology measures. We have been particularly interested in multimodal imaging methods that combine and leverage nanoscale spatial localization, visualization, biophysical and physico-chemical analysis

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