Burkholderia sordidicola sp. nov., isolated from the white-rot fungus Phanerochaete sordida.

Two bacterial strains associated with the white-rot fungus Phanerochaete sordida were subjected to taxonomic investigation. The isolates, designated KCTC 12081(T) and KCTC 12082, were Gram-negative, non-motile, non-spore-forming and ovoid to rod-shaped. The strains contained major amounts of hexadecanoic acid, cyclo-heptadecanoic acid and omega-7-cis-octadecenoic acid in their cell envelopes. Strain KCTC 12081(T) contained ubiquinone-8 as the major isoprenoid quinone and the G+C content of its genomic DNA was 61.3 mol%. Morphological and chemotaxonomic properties of the strains were consistent with classification in the genus BURKHOLDERIA: In a comparison of 16S rDNA sequence, KCTC 12081(T) shared 100 % similarity with KCTC 12082 and both strains formed a distinct phylogenetic lineage within the genus BURKHOLDERIA: The two strains were also differentiated from other species of this genus by fatty acid composition and phenotypic properties. DNA-DNA relatedness data further supported the separation of the new isolates from closely related species. It is therefore proposed that strains KCTC 12081(T) (=JCM 11778(T)) and KCTC 12082 be recognized as a novel species, for which the name Burkholderia sordidicola sp. nov. is proposed.

[1]  M. P. Cummings.  PHYLIP (Phylogeny Inference Package) , 2004 .

[2]  P. Vandamme,et al.  Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. , 2001, International journal of systematic and evolutionary microbiology.

[3]  P. Vandamme,et al.  Burkholderia fungorum sp. nov. and Burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples. , 2001, International journal of systematic and evolutionary microbiology.

[4]  R. Amann,et al.  Combined Use of 16S Ribosomal DNA and 16S rRNA To Study the Bacterial Community of Polychlorinated Biphenyl-Polluted Soil , 2001, Applied and Environmental Microbiology.

[5]  T. Cajthaml,et al.  Irpex lacteus, a white rot fungus applicable to water and soil bioremediation , 2000, Applied Microbiology and Biotechnology.

[6]  Y. Lim,et al.  The Aphyllophorales of Mungyong Saejae , 2000 .

[7]  D. M. Ward,et al.  Effect of Model Sorptive Phases on Phenanthrene Biodegradation: Molecular Analysis of Enrichments and Isolates Suggests Selection Based on Bioavailability , 2000, Applied and Environmental Microbiology.

[8]  V. Buonocore,et al.  Laccase from the white-rot fungus Trametes trogii , 1998, Applied Microbiology and Biotechnology.

[9]  P. Vandamme,et al.  Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. , 1997, International journal of systematic bacteriology.

[10]  E. Falsen,et al.  Bacteria Are Omnipresent on Phanerochaete chrysosporium Burdsall , 1996, Applied and environmental microbiology.

[11]  T. Heulin,et al.  Polyphasic Taxonomy in the Genus Burkholderia Leading to an Emended Description of the Genus and Proposition of Burkholderia vietnamiensis sp. nov. for N2-Fixing Isolates from Rice in Vietnam , 1995 .

[12]  R. Kondo,et al.  In Vitro Bleaching of Hardwood Kraft Pulp by Extracellular Enzymes Excreted from White Rot Fungi in a Cultivation System Using a Membrane Filter , 1994, Applied and environmental microbiology.

[13]  Takayuki Ezaki,et al.  Proposal of Burkholderia gen. nov. and Transfer of Seven Species of the Genus Pseudomonas Homology Group II to the New Genus, with the Type Species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. , 1992, Microbiology and immunology.

[14]  T. Kent Kirk,et al.  Sensitivity to and Degradation of Pentachlorophenol by Phanerochaete spp , 1990, Applied and environmental microbiology.

[15]  K. Hjortstam,et al.  The Corticiaceae of North Europe , 1977 .

[16]  P. Doty,et al.  Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. , 1962, Journal of molecular biology.

[17]  J. Parke,et al.  Diversity of the Burkholderia cepacia complex and implications for risk assessment of biological control strains. , 2001, Annual review of phytopathology.

[18]  Y. Yamada.  Identification of coenzyme Q (ubiquinone) homologs , 1998 .

[19]  D. Lane.  16S/23S rRNA sequencing , 1991 .

[20]  N. Palleroni.  Genus I. Pseudomonas Migula 1894 , 1984 .