Kinetics of the incorporation of tropoelastin into elastic fibers in embryonic chick aorta.

Matrix-free cells isolated by enzymic digestion of chick embryo aortas were labeled with [14C]proline for 20 to 60 min and the kinetics of the secretion of tropoelastin were followed by chasing the label and assaying [14C]tropoelastin in the cells and in the medium. The results indicated that secretion of tropoelastin followed the kinetics of a single first order process with a half time of 60 min. In parallel experiments tissue explants of chick embryo aortas were labeled with [14C]proline and the kinetics for the incorporation of tropoelastin into elastic fibers were followed by chasing the label and assaying the soluble [14C]tropoelastin and insoluble [14C]elastin in tissues. It was found that the incorporation of tropoelastin into elastic fibers also followed a single first order process with a half time of 85 min, similar to the secretion of tropoelastin from cells. In further studies, antibodies directed against tropoelastin were utilized to isolate soluble [14C]elastin components in the tissues after 0 to 4 hr chase of the 14C label. The results demonstrated that all soluble elastin components were recovered as monomeric tropoelastin and no soluble oligomeric elastin could be detected. These results are consistent with the proposition that elastic fiber growth occurs by addition of individual tropoelastin molecules to existing fibers and that oligomers of elastin were not intermediates in the process.

[1]  J. Foster,et al.  Elastin biosynthesis in chick embryonic lung tissue. Comparison to chick aortic elastin. , 1981, Biochemistry.

[2]  J. Foster,et al.  Translation of chick aortic elastin messenger ribonucleic acid. Comparison to elastin synthesis in chick aorta organ culture. , 1980, Biochemistry.

[3]  W. Kao,et al.  Kinetics for the secretion of nonhelical procollagen by freshly isolated tendon cells. , 1979, The Journal of biological chemistry.

[4]  J. Rosenbloom,et al.  Isolation and translation of elastin mRNA from chick aorta. , 1979, Biochemical and biophysical research communications.

[5]  D. Prockop,et al.  Synthesis of an elastin component of molecular weight about 70,000 by polysomes from chick embryo aortas. , 1978, Archives of biochemistry and biophysics.

[6]  R A Berg,et al.  Kinetics for the secretion of procollagen by freshly isolated tendon cells. , 1977, The Journal of biological chemistry.

[7]  D. Prockop,et al.  Synthesis of elastin in aortas from chick embryos. Conversion of newly secreted elastin to cross-linked elastin without apparent proteolysis of the molecule. , 1977, Biochemistry.

[8]  B. Olsen,et al.  Interchain disulfide bonds at the COOH-terminal end of procollagen synthesized by matrix-free cells from chick embryonic tendon and cartilage. , 1976, Archives of biochemistry and biophysics.

[9]  J. Rosenbloom,et al.  Inhibition of proline hydroxylation does not inhibit secretion of tropoelastin by chick aorta cells , 1976, FEBS letters.

[10]  J. Rosenbloom,et al.  Biosynthesis and secretion of tropoelastin by chick aorta cells. , 1976, Biochemical and biophysical research communications.

[11]  J. Uitto,et al.  Synthesis of elastin and procallagen by cells from embryonic aorta. Differences in the role of hydroxyproline and the effects of proline analogs on the secretion of the two proteins. , 1976, Archives of biochemistry and biophysics.

[12]  R. Page,et al.  Demonstration of a precursor-product relationship between soluble and cross-linked elastin, and the biosynthesis of the desmosines in vitro. , 1976, The Journal of biological chemistry.

[13]  R. Shapiro,et al.  Isolation of soluble elastin from lathyritic chicks. Comparison to tropoelastin from copper deficient pigs. , 1975, Biochemistry.

[14]  M. Haniu,et al.  Chick tropoelastin. Isolation and partial chemical characterization. , 1975, Biochemical and biophysical research communications.

[15]  L. Sandberg,et al.  A new method for purification of mature elastin. , 1975, Analytical biochemistry.

[16]  L. Sandberg,et al.  Tropoelastin purification: improvements using enzyme inhibitors. , 1975, Analytical biochemistry.

[17]  B. Sykes,et al.  Salt-soluble elastin from lathyritic chicks. , 1974, The Biochemical journal.

[18]  D. W. Smith,et al.  Synthesis of soluble elastin by aortic medial cells in culture. , 1974, Biochemical and biophysical research communications.

[19]  D. W. Smith,et al.  Preparation and properties of salt-soluble elastin. , 1972, The Journal of biological chemistry.

[20]  J. King,et al.  Polypeptides of the tail fibres of bacteriophage T4. , 1971, Journal of molecular biology.

[21]  G. Martin,et al.  Cross-linking of collagen and elastin. Properties of lysyl oxidase. , 1970, Biochemistry.

[22]  D. W. Smith,et al.  The purification and partial characterization of a soluble elastin-like protein from copper-deficient porcine aorta. , 1969, Biochemistry.

[23]  E. Dempsey,et al.  The structure and chemical characterization of elastic fibers as reveled by elastase and by electron microscopy , 1952, The Anatomical record.

[24]  W. Kao,et al.  Demonstration by immunofluorescence that the same cells from chick embryo aortas synthesize elastin and collagen types I and III. , 1980, Artery.

[25]  P. Jones,et al.  Glycoprotein, elastin, and collagen secretion by rat smooth muscle cells. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[26]  L. Fessler,et al.  Biosynthesis of procollagen. , 1978, Annual review of biochemistry.

[27]  J. Uitto,et al.  Intracellular Steps in the Biosynthesis of Collagen , 1976 .