Fibroblast contraction of a collagen-GAG matrix.

Contractile cells, found in wounded or diseased tissues, are associated with the formation of scar tissue. The complexity of contraction in vivo has led to the development of models of contraction by cells in vitro. In this work, a device was developed which quantitatively measured the contractile force developed by fibroblasts seeded into a collagen-glycosaminoglycan porous matrix in vitro. This device differed from most of those previously described in that it directly transferred cellular contractile force to the force transducer (a cantilever beam) and that it used a porous matrix rather than a collagen gel. The data for the increase in contractile force with time were fit to a mathematical equation using two fitting parameters. Data were then compared using the fitting parameters and the cell density. A study of the effect of cell density on the contractile force resulted in a linearly proportional relationship. Subsequent normalization of force by cell density or number resulted in a value of contractile force per cell, 1 nN, that was independent of cell density. The time for the contractile force to develop was also independent of cell density. These results suggest that, in this system, cells develop contractile force individually, irrespective of the force generated by surrounding cells.

[1]  E Bell,et al.  Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[2]  M Eastwood,et al.  Tensional homeostasis in dermal fibroblasts: Mechanical responses to mechanical loading in three‐dimensional substrates , 1998, Journal of cellular physiology.

[3]  Mark Eastwood,et al.  Quantitative analysis of collagen gel contractile forces generated by dermal fibroblasts and the relationship to cell morphology , 1996, Journal of cellular physiology.

[4]  Ioannis V. Yannas,et al.  Biologically Active Analogues of the Extracellular Matrix: Artificial Skin and Nerves† , 1990 .

[5]  M. Spector,et al.  Tendon cell contraction of collagen-GAG matrices in vitro: effect of cross-linking. , 2000, Biomaterials.

[6]  Richard A.F. Clark,et al.  The Molecular and Cellular Biology of Wound Repair , 2012, Springer US.

[7]  M Eastwood,et al.  A culture force monitor for measurement of contraction forces generated in human dermal fibroblast cultures: evidence for cell-matrix mechanical signalling. , 1994, Biochimica et biophysica acta.

[8]  M S Kolodney,et al.  Isometric contraction by fibroblasts and endothelial cells in tissue culture: a quantitative study , 1992, The Journal of cell biology.

[9]  I. Yannas,et al.  Design of an artificial skin. II. Control of chemical composition. , 1980, Journal of biomedical materials research.

[10]  O. Petersen,et al.  A function for filamentous alpha-smooth muscle actin: retardation of motility in fibroblasts , 1996, The Journal of cell biology.

[11]  A. Desmoulière,et al.  The Role of the Myofibroblast in Wound Healing and Fibrocontractive Diseases , 1988 .

[12]  P. Agache,et al.  A mechanical study of tense collagen lattices , 1996 .

[13]  A. Desmoulière,et al.  α-Smooth muscle actin is expressed in a subpopulation of cultured and cloned fibroblasts and is modulated by γ-interferon☆ , 1992 .

[14]  L. Schmidt,et al.  Activation of a muscle-specific actin gene promoter in serum-stimulated fibroblasts. , 1992, Molecular biology of the cell.

[15]  Edward Y Lee,et al.  Synthesis and characterization of a model extracellular matrix that induces partial regeneration of adult mammalian skin. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[16]  A. Harris,et al.  Silicone rubber substrata: a new wrinkle in the study of cell locomotion. , 1980, Science.

[17]  H. D. Cavanagh,et al.  An in vitro force measurement assay to study the early mechanical interaction between corneal fibroblasts and collagen matrix. , 1997, Experimental cell research.

[18]  M. Spector,et al.  Meniscus cells seeded in type I and type II collagen-GAG matrices in vitro. , 1999, Biomaterials.

[19]  J. Lévêque,et al.  Measurement of mechanical forces generated by skin fibroblasts embedded in a three-dimensional collagen gel. , 1991, The Journal of investigative dermatology.

[20]  M Eastwood,et al.  Balanced mechanical forces and microtubule contribution to fibroblast contraction , 1996, Journal of cellular physiology.

[21]  Frederick Grinnell,et al.  Fibroblasts, myofibroblasts, and wound contraction , 1994, The Journal of cell biology.