GFP expression in the mammary gland for imaging of mammary tumor cells in transgenic mice.

To examine the behavior of tumor cells in tumors developing directly from mammary tissue in transgenic models, we have evaluated transgenic mice expressing green fluorescent protein (GFP). Using the mouse mammary virus promoter (MMTV) to directly drive expression of GFP, we find low levels of fluorescence in the mammary and salivary glands of transgenic animals. Using MMTV-Cre or WAP-Cre in combination with the Cre-activatable CAG-CAT-EGFP construct, we find stronger expression of GFP that is still tissue specific. These animals provide a range of expression of GFP that is suitable for analysis of transgenic mammary tumors and metastases in vivo at the single cell level of resolution.

[1]  J. Zavadil,et al.  Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. , 2002, Cancer research.

[2]  P. Seeburg,et al.  Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studies , 2001, Progress in Neurobiology.

[3]  J. Miyazaki,et al.  Usefulness of double gene construct for rapid identification of transgenic mice exhibiting tissue‐specific gene expression , 2001, Molecular reproduction and development.

[4]  L. Hennighausen,et al.  Spatial and temporal expression of the Cre gene under the control of the MMTV-LTR in different lines of transgenic mice , 2001, Transgenic Research.

[5]  John N. Hutchinson,et al.  Transgenic mouse models of human breast cancer , 2000, Oncogene.

[6]  Robert M Hoffman,et al.  Green fluorescent protein imaging of tumor cells in mice. , 2002, Lab animal.

[7]  M. Moran,et al.  Grb2 and Shc Adapter Proteins Play Distinct Roles in Neu (ErbB-2)-Induced Mammary Tumorigenesis: Implications for Human Breast Cancer , 2001, Molecular and Cellular Biology.

[8]  Andras Nagy,et al.  The color of mice: in the light of GFP-variant reporters , 2001, Histochemistry and Cell Biology.

[9]  Elise C. Kohn,et al.  The microenvironment of the tumour–host interface , 2001, Nature.

[10]  Tomoko Nakanishi,et al.  ‘Green mice’ as a source of ubiquitous green cells , 1997, FEBS letters.

[11]  William A Mohler,et al.  Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues. , 2002, Biophysical journal.

[12]  G. Naumov,et al.  Persistence of solitary mammary carcinoma cells in a secondary site: a possible contributor to dormancy. , 2002, Cancer research.

[13]  L. Hennighausen,et al.  Cre-mediated gene deletion in the mammary gland. , 1997, Nucleic acids research.

[14]  J. Segall,et al.  Imaging of cancer invasion and metastasis using green fluorescent protein. , 2000, European journal of cancer.

[15]  Andrew V. Nguyen,et al.  Colony-Stimulating Factor 1 Promotes Progression of Mammary Tumors to Malignancy , 2001, The Journal of experimental medicine.

[16]  J. Miyazaki,et al.  A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre‐mediated recombination , 2000, FEBS letters.

[17]  H. Gabbert,et al.  Mechanisms of tumor metastasis: cell biological aspects and clinical implications , 2000, Journal of Cancer Research and Clinical Oncology.

[18]  R. Cardiff,et al.  Mammary Disease Mice Model Premalignant Polyoma Middle-T Transgenic Updated Version , 2001 .

[19]  J. Segall,et al.  Lamellipodia in invasion. , 2001, Seminars in cancer biology.

[20]  Christopher W. Wong,et al.  Apoptosis: an early event in metastatic inefficiency. , 2001, Cancer research.

[21]  J. Segall,et al.  A critical step in metastasis: in vivo analysis of intravasation at the primary tumor. , 2000, Cancer research.