Enzyme-free amplified SERS immunoassay for the ultrasensitive detection of disease biomarkers.

We developed a novel enzyme-free amplified SERS immunoassay by combining silver nanoparticle (AgNP)-linked immunoreaction and SERS transduction for the detection of disease biomarkers. As a proof of concept, our method was successfully illustrated with the disease biomarker α-fetoprotein with the detection limit of 3.3  ×  10-13 g mL-1 and a double-blind experiment consisting of tens of serum samples was performed to confirm its reliability.

[1]  S. Gray,et al.  Self-assembled large Au nanoparticle arrays with regular hot spots for SERS. , 2011, Small.

[2]  Minghui Yang,et al.  A label-free electrochemical immunosensor for the detection of cancer biomarker α-fetoprotein (AFP) based on hydroxyapatite induced redox current , 2016 .

[3]  X. Qu,et al.  Cancer biomarker detection: recent achievements and challenges. , 2015, Chemical Society reviews.

[4]  Chao Ma,et al.  Detection of α-fetoprotein with an ultrasensitive electrochemiluminescence paper device based on green-luminescent nitrogen-doped graphene quantum dots , 2015 .

[5]  X. Bi,et al.  Silver-catalysed reactions of alkynes: recent advances. , 2015, Chemical Society reviews.

[6]  S. Sim,et al.  A Nanoplasmonic Biosensor for Ultrasensitive Detection of Alzheimer's Disease Biomarker Using a Chaotropic Agent. , 2019, ACS sensors.

[7]  Xiangwei Zhao,et al.  Vertical flow assays based on core-shell SERS nanotags for multiplex prostate cancer biomarker detection. , 2019, The Analyst.

[8]  Zhilin Yang,et al.  Probing the Location of 3D Hot Spots in Gold Nanoparticle Films Using Surface-Enhanced Raman Spectroscopy. , 2019, Analytical chemistry.

[9]  Ping Liu,et al.  High-performance fluorescence-encoded magnetic microbeads as microfluidic protein chip supports for AFP detection. , 2016, Analytica chimica acta.

[10]  Qingyun Liu,et al.  A novel strategy for amplified probing versatile biomolecules through a photoswitchable biocatalytic cascade , 2018, Sensors and Actuators B: Chemical.

[11]  Yiping Chen,et al.  Manganese dioxide nanoparticle-based colorimetric immunoassay for the detection of alpha-fetoprotein , 2017, Microchimica Acta.

[12]  Hongyang Wang,et al.  Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum , 2016, Theranostics.

[13]  S. A. Kandeal,et al.  Assessment Techniques to Detect Aspergillus fumigatus in Different Samples of Immunosuppressed Male Western Albino Rats , 2014, Jundishapur journal of microbiology.

[14]  Gang Liu,et al.  High-sensitivity nanosensors for biomarker detection. , 2012, Chemical Society reviews.

[15]  Paramita Karfa,et al.  A battle between spherical and cube-shaped Ag/AgCl nanoparticle modified imprinted polymer to achieve femtogram detection of alpha-feto protein. , 2016, Journal of materials chemistry. B.

[16]  Xiaolin Fu,et al.  Chemiluminescence enzyme immunoassay using magnetic nanoparticles for detection of neuron specific enolase in human serum. , 2012, Analytica chimica acta.

[17]  H. Tian,et al.  Sensitive detection of protein biomarkers using silver nanoparticles enhanced immunofluorescence assay , 2017, Theranostics.

[18]  Zhongze Gu,et al.  Quantitative and ultrasensitive detection of multiplex cardiac biomarkers in lateral flow assay with core-shell SERS nanotags. , 2018, Biosensors & bioelectronics.

[19]  Namhyun Choi,et al.  Simultaneous Detection of Dual Prostate Specific Antigens Using Surface-Enhanced Raman Scattering-Based Immunoassay for Accurate Diagnosis of Prostate Cancer. , 2017, ACS nano.

[20]  Shuvashis Dey,et al.  Electrohydrodynamic-Induced SERS Immunoassay for Extensive Multiplexed Biomarker Sensing. , 2017, Small.

[21]  Jing Zhu,et al.  Cascade Signal Amplification Based on Copper Nanoparticle-Reported Rolling Circle Amplification for Ultrasensitive Electrochemical Detection of the Prostate Cancer Biomarker. , 2016, ACS applied materials & interfaces.

[22]  Kenichi Yoshida,et al.  Evaluation of electromagnetic enhancement of surface enhanced hyper Raman scattering using plasmonic properties of binary active sites in single Ag nanoaggregates. , 2009, The Journal of chemical physics.

[23]  Yong-bok Choi,et al.  Comparisons of ELISA and Western blot assays for detection of autophagy flux , 2017, Data in brief.

[24]  Feifei Zhang,et al.  A graphene oxide-based label-free electrochemical aptasensor for the detection of alpha-fetoprotein. , 2018, Biosensors & bioelectronics.

[25]  Julian Moger,et al.  Clinical applications of infrared and Raman spectroscopy: state of play and future challenges. , 2018, The Analyst.

[26]  M. Tokeshi,et al.  Ultrasensitive detection of disease biomarkers using an immuno-wall device with enzymatic amplification. , 2019, The Analyst.

[27]  Z. Shen,et al.  Highly ordered arrays of particle-in-bowl plasmonic nanostructures for surface-enhanced raman scattering. , 2012, Small.

[28]  Y. Long,et al.  Alcohol Dehydrogenase-Catalyzed Gold Nanoparticle Seed-Mediated Growth Allows Reliable Detection of Disease Biomarkers with the Naked Eye. , 2015, Analytical chemistry.

[29]  Y. Long,et al.  Redox-Mediated Indirect Fluorescence Immunoassay for the Detection of Disease Biomarkers Using Dopamine-Functionalized Quantum Dots. , 2016, Analytical chemistry.

[30]  Y. Kondo,et al.  Significant biomarkers for the management of hepatocellular carcinoma , 2015, Clinical Journal of Gastroenterology.

[31]  S. S. Sinha,et al.  Nanoarchitecture Based SERS for Biomolecular Fingerprinting and Label-Free Disease Markers Diagnosis , 2016, Accounts of chemical research.

[32]  Nianqiang Wu,et al.  A Hierarchically Ordered Array of Silver‐Nanorod Bundles for Surface‐Enhanced Raman Scattering Detection of Phenolic Pollutants , 2016, Advanced materials.

[33]  D. Tang,et al.  In situ synthesis of fluorescent polydopamine nanoparticles coupled with enzyme-controlled dissolution of MnO2 nanoflakes for a sensitive immunoassay of cancer biomarkers. , 2017, Journal of materials chemistry. B.

[34]  Taihong Wang,et al.  Positive potential operation of a cathodic electrogenerated chemiluminescence immunosensor based on luminol and graphene for cancer biomarker detection. , 2011, Analytical chemistry.

[35]  Chao Tian,et al.  Cellular imaging by targeted assembly of hot-spot SERS and photoacoustic nanoprobes using split-fluorescent protein scaffolds , 2018, Nature Communications.

[36]  Rashida Akter,et al.  Amplified electrochemical detection of a cancer biomarker by enhanced precipitation using horseradish peroxidase attached on carbon nanotubes. , 2012, Analytical chemistry.