Rapid measurement of impedance changes associated with developmental phases in Artemia cysts

A rapidly prototyped microfluidic device made of glass microscope slides and polydimethylsiloxane (PDMS) was developed to characterize impedance changes during the developmental stages of Artemia cysts. The device was designed to measure the impedance of a single cyst. The device performance was tested by measuring the impedance of Artemia cysts until they hatched. The measured impedance corresponded to physiological changes as the cyst developed.

[1]  T. MacRae.  Stress tolerance during diapause and quiescence of the brine shrimp, Artemia , 2015, Cell Stress and Chaperones.

[2]  N. Gostling,et al.  ONTOGENY AND TAPHONOMY: AN EXPERIMENTAL TAPHONOMY STUDY OF THE DEVELOPMENT OF THE BRINE SHRIMP ARTEMIA SALINA , 2009 .

[3]  Junbo Wang,et al.  Microfluidic Impedance Flow Cytometry Enabling High-Throughput Single-Cell Electrical Property Characterization , 2015, International journal of molecular sciences.

[4]  J. Clegg.  Protein Stability in Artemia Embryos During Prolonged Anoxia , 2007, The Biological Bulletin.

[5]  Luis Alvarez,et al.  Identification of mesenchymal stem cell differentiation state using dual-micropore microfluidic impedance flow cytometry , 2016 .

[6]  S. Gawad,et al.  Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing. , 2001, Lab on a chip.

[7]  Nicole Bock,et al.  Label-free whole blood cell differentiation based on multiple frequency AC impedance and light scattering analysis in a micro flow cytometer. , 2016, Lab on a chip.

[8]  Patrick Sorgeloos,et al.  Manual on the production and use of live food for aquaculture , 1996 .

[9]  S. Gawad,et al.  Impedance spectroscopy flow cytometry: On‐chip label‐free cell differentiation , 2005, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[10]  G. Careri,et al.  Dielectric properties of Artemia cysts at low water contents. Evidence for a percolative transition. , 1989, Biophysical Journal.

[11]  S. Hand,et al.  Physiological strategies during animal diapause: lessons from brine shrimp and annual killifish , 2015, The Journal of Experimental Biology.

[12]  Hongkai Wu,et al.  Recent Developments in Microfluidics for Cell Studies , 2014, Advanced materials.

[13]  T. Turner,et al.  Effect of submicrosecond electric fields on microorganisms: experiments and applications , 1995, Photonics West.