Callus Induction and Plant Regeneration from Node Explants of Ziziphus Spina- Christi

Ziziphus spina -christi (L.) ecologically and economically important due to its tolerance to drought and salinity. The purpose of this study was developing a standardized protocol to induce a high frequency of callus in Z. spina -christi for breeding programs with the final objective of plantlet regeneration from node explants. Node explants of aseptic seedlings, were cultured on MS medium containing the hormone, such as 2, 4-D, NAA, TDZ and BAP. For regeneration, different levels of the PGRs including BAP, TDZ and Kin were applied. Also rooting of seedlings obtained from 1/2MS medium, were performed in the presence of IBA and NAA. Results showed that culture medium containing 2, 4-D and TDZ at concentrations of 0.5 and 1 mg/l ( ) has the highest amount of callus induction. Most of the node explants cultured on BAP medium were producing direct shoot at callusing phase. 5 mg/l of Kin and 1 mg/l of TDZ respectively, had the highest average shoot with numerical average of 84.8, 95.8 but the shoot produced by TDZ had an unusual shape but applying Kin, direct normal shoots were produced. The value of root produced by the hormone IBA more than NAA at all concentrations. KeywordsCallus Induction; Growth Regulators; Node Explants; Ziziphus Spina -Christi

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