Ultrastructural and Immunohistochemical Studies of Transplanted Canine Lung Carcinoma Cell to Severe Combined Immunodeficiency Mice (STUDI ULTRASTRUKTUR DAN IMUNOHISTOKIMIA TRANSPLANTASI SEL KANKER PARU-PARU ANJING PADA MENCIT SEVERE COMBINED IMMUNODEFF

Primary lung cancers, or tumors originating in the lung, are relatively uncommon in dogs. The objective of this study was to describe the canine lung carcinoma that serially transplanted into severe combined immunodeficiency (SCID) mice, in order to established cell line from this tumor cell. Morphology and characteristic of this canine lung carcinoma in SCID mice by histopathological and ultrastructural examinations with metastatic lesion in lung were also examined. Histopathologically, the tumor mass were consisted of cuboidal to columnar cells with papillary pattern, uniform in size, the nuclei were often variable in size, and some cells have vacuole on their cytoplasms. Glandular forms were predominant with lobulated pattern, ductal pattern with papillary injected into tube-like structure were also encountered. Mitotic figures commonly found with inflammatory reaction were sometimes present in the interstitium and lumen gland. Ultrastructural analysis of the tumor cells showed round to oval cells with one or more prominent nucleoli. The cells possessed numerous mitochondria, smooth endoplasmic reticulum, and individual cells which were interconnected via desmosomes. Tonofilament characterize by long cytoplasmic material was encountered. Positive reaction of the round to oval tumor cells to anti keratin antibody confirmed that their epithelial cell nature. Lung metastatic lesions were found in SCID mice after transplantation and this phenomenon indicated that canine lung carcinoma is tumorigenic to SCID mice.