INVESTIGATION OF TWO MODELS OF TRINITROBENZENESULFONIC ACID-INDUCED COLITIS IN RATS

PURPOSE: Тhe aim of this study was to comparatively evaluate rat experimental colitis resulting from administration of two different doses of trinitrobenzenesulfonic acid (TNBS) dissolved in different volumes of two ethanol concentrations. METHODS: TNBS was applied in the colon by a soft cannula at a depth of about 10 cm from anus. The first group (TNBS20) was treated with 20 mg TNBS dissolved in 0.4 ml of 40% ethanol solution. The second group (TNBS10) was treated with 10 mg TNBS dissolved in 0.25 ml of 50% ethanol solution. Тhe animals were sacrificed 24 hours after the treatment and the colon was examined macroscopically and histopathologically. RESULTS: There were no significant differences regarding the degree of hyperemia, total area of injury and thickening of the colon wall. The higher TNBS dose produced significantly more pronounced shortening of the colon and larger necrotic area. Histopathological evaluation showed similar degree of injuries in the two models. CONCLUSION: The two investigated models of TNBS-induced colitis were comparable according to the overall macroscopic and microscopic criteria for colitis evaluation.