1α,25-dihydroxyvitamin D3 stimulated alkaline phosphatase activity in cultured pig kidney epithelial LLC-PK1 cells

The effects of 1α,25-dihydroxyvitamin D 3 (1.25 (OH) 2 D 3 ) and human growth hormone (hGH) on the activity of alkaline phosphatase (ALP) were evaluated in pig kidney LLC-PK1 cultured cells. 1.25(OH) 2 D 3 (10 -9 -10 -6 mol L -1 ) and hGH (10 -7 mol L -1 ) increased ALP activity in these cells while no hormonal effect was detected on two other enzyme activities : γ-glutamyl transferase and acid phosphatase. ALP activity was maximally increased after 4.5-6 h incubation with both hormones The hormonal induction of ALP activity was prevented by a pretreatment of cells with actinomycin D. Thus, 1.25 (OH) 2 D 3 and may be hGH could stimulate ALP activity via a transcription of some gene.