Multipurpose electrode with different enzyme systems bound to collagen films

Abstract Selective, multipurpose electrodes have been developed from the previously described glucose electrode based on amperometric detection of hydrogen peroxide. Several single or multi-enzyme systems, including galactose oxidase, cholesterol oxidase, glucoamylase with glucose oxidase, and invertase with glucose oxidase, can be covalently bound to collagen membranes and attached to a platinum anode for monitoring the hydrogen peroxide generated. The probes allow fast and sensitive measurements of galactose, free cholesterol and maltose. Analogous electrodes are convenient for the assay of sucrose and lactose, with lower sensitivity. For disaccharide measurements, a comparative study of membranes produced by random co-immobilization, stacking of membranes and asymmetric coupling is reported. Asymmetric coupling improved the electrode performances in every case. One enzyme membrane is readily replaced by another in the electrode construction, and the sensors can be used for hundreds of assays.

[1]  L. C. Clark,et al.  ELECTRODE SYSTEMS FOR CONTINUOUS MONITORING IN CARDIOVASCULAR SURGERY , 1962 .

[2]  P. Coulet,et al.  A mild method of general use for covalent coupling of enzymes to chemically activated collagen films , 1974, Biotechnology and bioengineering.

[3]  J. Brillouet,et al.  Thin layer‐flow reactor with amyloglucosidase bound to collagen membranes , 1976 .

[4]  P. Coulet,et al.  Asymmetrical Coupling of Enzymatic Systems on Collagen Membranes Application to Multienzyme Electrodes , 1979 .

[5]  I. Karube,et al.  Enzyme electrode for free cholesterol. , 1977, Biotechnology and bioengineering.

[6]  D. Thomas,et al.  Magnetic enzyme membranes as active elements of electrochemical sensors. Lactose, saccharose, maltose bienzyme electrodes , 1975, FEBS Letters.

[7]  G. Guilbault,et al.  Amperometric determination of total cholesterol in serum, with use of immobilized cholesterol ester hydrolase and cholesterol oxidase. , 1977, Clinical chemistry.

[8]  B. Horecker,et al.  The D-galactose oxidase of Polyporus circinatus. , 1962, The Journal of biological chemistry.

[9]  G. Lubrano,et al.  Amperometric enzyme electrodes: Part III. Alcohol oxidase , 1974 .

[10]  I. Silman,et al.  Water-insoluble derivatives of enzymes, antigens, and antibodies. , 1966, Annual review of biochemistry.

[11]  P. Coulet,et al.  Enzyme Electrode with Collagen-Immobilized Cholesterol Oxidase for the Microdetermination of Free Cholesterol , 1979 .

[12]  P. Taylor,et al.  Design, construction, and applications of a galactose selective electrode. , 1977, Analytical chemistry.

[13]  I. Karube,et al.  Enzyme electrode for sucrose. , 1976, Biotechnology and bioengineering.

[14]  Robert Sternberg,et al.  ENZYME COLLAGEN MEMBRANE FOR ELECTROCHEMICAL DETERMINATION OF GLUCOSE , 1979 .

[15]  M. H. Sadar,et al.  Preparation and analytical uses of immobilized enzymes , 1979 .