Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.

[1]  Thomas C Evans,et al.  Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes. , 2015, BioTechniques.

[2]  Malik Peiris,et al.  Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia , 2020, Clinical chemistry.

[3]  A. M. Leontovich,et al.  The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2 , 2020, Nature Microbiology.

[4]  P. Vollmar,et al.  Transmission of 2019-nCoV Infection from an Asymptomatic Contact in Germany , 2020, The New England journal of medicine.

[5]  Yuntao Wu.  Compensation of ACE2 Function for Possible Clinical Management of 2019-nCoV-Induced Acute Lung Injury , 2020, Virologica Sinica.

[6]  K. Yuen,et al.  Clinical Characteristics of Coronavirus Disease 2019 in China , 2020, The New England journal of medicine.

[7]  E. Holmes,et al.  A new coronavirus associated with human respiratory disease in China , 2020, Nature.

[8]  E. Holmes,et al.  Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding , 2020, The Lancet.

[9]  R. Chiu,et al.  Racing Towards the Development of Diagnostics for a Novel Coronavirus (2019-nCoV) , 2020, Clinical chemistry.

[10]  G. Gao,et al.  A Novel Coronavirus from Patients with Pneumonia in China, 2019 , 2020, The New England journal of medicine.

[11]  Shibo Jiang,et al.  The First Disease X is Caused by a Highly Transmissible Acute Respiratory Syndrome Coronavirus , 2020, Virologica Sinica.

[12]  Jing Zhao,et al.  Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus–Infected Pneumonia , 2020, The New England journal of medicine.

[13]  G. Leung,et al.  Nowcasting and forecasting the potential domestic and international spread of the 2019-nCoV outbreak originating in Wuhan, China: a modelling study , 2020, The Lancet.

[14]  X. Xia,et al.  A Mismatch-Tolerant Reverse Transcription Loop-Mediated Isothermal Amplification Method and Its Application on Simultaneous Detection of All Four Serotype of Dengue Viruses , 2019, Front. Microbiol..

[15]  Chiyu Zhang,et al.  A Mismatch-tolerant RT-LAMP Method for Molecular Diagnosis of Highly Variable Viruses. , 2019, Bio-protocol.

[16]  Kai Zhao,et al.  A pneumonia outbreak associated with a new coronavirus of probable bat origin , 2020, Nature.