Freeze-fracture of lipids and model membrane systems.

Model membrane systems are used extensively in all aspects of membrane research, and freeze-fracture is the preeminent procedure for directly visualizing local structure in these lipid dispersions. Here we describe in detail the formation of liposomes and how freeze-fracture is routinely employed as a complementary technique to biophysical and biochemical procedures in the characterization of multilamellar vesicles (most commonly known as liposomes) and unilamellar vesicles. Many preparative procedures exist for the formation of multi- and unilamellar vesicles. Examples of each system are given and their properties as well as freeze-fracture morphology are discussed. The detection of lipid-phase transitions is considered, in particular, with emphasis on the application of freeze-fracture to the study of lipid polymorphism. We briefly discuss the fracturing of apolar lipids which do not adopt bilayer structures but which can be stabilized into microemulsions by a phospholipid monolayer. Finally, a critical assessment is made of filipin as a morphological marker for cholesterol domains in the plane of the bilayer.

[1]  P. Hanawalt,et al.  Isolation of DNA replication complexes from uninfected and adenovirus-infected HeLa cells. , 1971, Journal of molecular biology.

[2]  H. Robenek,et al.  Detection of microdomains in biomembranes. An appraisal of recent developments in freeze-fracture cytochemistry. , 1983, Biochimica et biophysica acta.

[3]  T. Reese,et al.  Phospholipid bilayer deformations associated with interbilayer contact and fusion , 1981, Nature.

[4]  A. Verkleij,et al.  Propane jet‐freezing: A valid ultra‐rapid freezing method for the preservation of temperature dependent lipid phases , 1981, Journal of microscopy.

[5]  B. de Kruyff,et al.  The function of sterols in membranes. , 1976, Biochimica et biophysica acta.

[6]  S. Gruner,et al.  Cation-dependent segregation phenomena and phase behavior in model membrane systems containing phosphatidylserine: influence of cholesterol and acyl chain composition. , 1984, Biochemistry.

[7]  L. Mayer,et al.  Vesicles of variable sizes produced by a rapid extrusion procedure. , 1986, Biochimica et biophysica acta.

[8]  D. Deamer,et al.  Permeability of lipid bilayers to water and ionic solutes. , 1986, Chemistry and physics of lipids.

[9]  B. Menco A survey of ultra‐rapid cryofixation methods with particular emphasis on applications to freeze‐fracturing, freeze‐etching, and freeze‐substitution , 1986 .

[10]  P. Quinn,et al.  Formation of inverted lipid micelles in aqueous dispersions of mixed sn-3-galactosyldiacylglycerols induced by heat and ethylene glycol. , 1982, Biochimica et biophysica acta.

[11]  G. Ginsburg,et al.  Microemulsions of phospholipids and cholesterol esters. Protein-free models of low density lipoprotein. , 1982, The Journal of biological chemistry.

[12]  F. Szoka,et al.  Comparative properties and methods of preparation of lipid vesicles (liposomes). , 1980, Annual review of biophysics and bioengineering.

[13]  S. Gruner,et al.  Polymorphic phase behavior of unsaturated lysophosphatidylethanolamines: a 31P NMR and X-ray diffraction study. , 1986, Biochemistry.

[14]  A. Verkleij,et al.  Lipidic intramembranous particles. , 1984, Nature.

[15]  A. Verkleij,et al.  Size determination of sonicated vesicles by freeze‐fracture electron microscopy, using the spray‐freezing method , 1980 .

[16]  S. Singer,et al.  The Fluid Mosaic Model of the Structure of Cell Membranes , 1972, Science.

[17]  A. Bangham,et al.  Diffusion of univalent ions across the lamellae of swollen phospholipids. , 1965, Journal of molecular biology.

[18]  L. Staehelin,et al.  Effects of reconstitution method on the structural organization of isolated chloroplast membrane lipids , 1984 .

[19]  B. van Deurs,et al.  Filipin as a cholesterol probe. II. Filipin-cholesterol interaction in red blood cell membranes. , 1984, European journal of cell biology.

[20]  T. P. Stewart,et al.  Membrane fusion through point defects in bilayers. , 1981, Science.

[21]  D. Friend,et al.  Influence of the membrane undercoat on filipin perturbation of the red blood cell membrane. , 1987, Experimental cell research.

[22]  F. Szoka,et al.  Preparation of liposomes of defined size distribution by extrusion through polycarbonate membranes. , 1979, Biochimica et biophysica acta.

[23]  S. Gruner,et al.  Lipid polymorphism: the molecular basis of nonbilayer phases. , 1985, Annual review of biophysics and biophysical chemistry.

[24]  S M Gruner,et al.  Novel multilayered lipid vesicles: comparison of physical characteristics of multilamellar liposomes and stable plurilamellar vesicles. , 1985, Biochemistry.

[25]  H. Mcconnell,et al.  Multiple phase equilibria in binary mixtures of phospholipids. , 1978, Biochimica et biophysica acta.

[26]  P. Ververgaert,et al.  Spray-freezing of liposomes. , 1973, Biochimica et biophysica acta.

[27]  Rodman G. Miller Do ‘lipidic particles’ represent intermembrane attachment sites? , 1980, Nature.

[28]  M. Sheetz,et al.  Effect of sonication on the structure of lecithin bilayers. , 1972, Biochemistry.

[29]  R. Miller,et al.  The use and abuse of filipin to localize cholesterol in membranes. , 1984, Cell biology international reports.

[30]  Lipid polymorphism and the functional roles of lipids in biological membranes. , 1979 .

[31]  J. Davis,et al.  The influence of membrane proteins on lipid dynamics. , 1986, Chemistry and physics of lipids.

[32]  K. Jacobson,et al.  Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles. , 1975, Biochimica et biophysica acta.

[33]  M. Bally,et al.  Generation of multilamellar and unilamellar phospholipid vesicles , 1986 .

[34]  P. Cullis,et al.  Effects of divalent cations and pH on phosphatidylserine model membranes: a 31P NMR study. , 1980, Biochemical and biophysical research communications.

[35]  J. Bolard How do the polyene macrolide antibiotics affect the cellular membrane properties? , 1986, Biochimica et biophysica acta.

[36]  G. Lindblom,et al.  Reversed cubic phase with membrane glucolipids from Acholeplasma laidlawii. 1H, 2H, and diffusion nuclear magnetic resonance measurements. , 1981, Biochemistry.

[37]  B. van Deurs,et al.  Filipin as a cholesterol probe. I. Morphology of filipin-cholesterol interaction in lipid model systems. , 1984, European journal of cell biology.

[38]  L. Mayer,et al.  Solute distributions and trapping efficiencies observed in freeze-thawed multilamellar vesicles. , 1985, Biochimica et biophysica acta.

[39]  D. Friend,et al.  Fusion of phospholipid vesicles arrested by quick-freezing. The question of lipidic particles as intermediates in membrane fusion. , 1982, Biochimica et biophysica acta.

[40]  Charles Tanford,et al.  The Hydrophobic Effect: Formation of Micelles and Biological Membranes , 1991 .

[41]  L. Mayer,et al.  Detection of protein-free lipoprotein analogues with an apolar lipid core by freeze-etch electron microscopy. , 1987, Biochimica et biophysica acta.

[42]  R. Rand Interacting phospholipid bilayers: measured forces and induced structural changes. , 1981, Annual review of biophysics and bioengineering.

[43]  M. Bally,et al.  Production of large unilamellar vesicles by a rapid extrusion procedure: characterization of size distribution, trapped volume and ability to maintain a membrane potential. , 1985, Biochimica et biophysica acta.

[44]  B. Lentz,et al.  Phase behavior of large unilamellar vesicles composed of synthetic phospholipids. , 1984, Biochemistry.

[45]  A. Verkleij,et al.  Phase transitions of phospholipid bilayers and membranes of Acholeplasma laidlawii B visualized by freeze fracturing electron microscopy. , 1972, Biochimica et biophysica acta.

[46]  P. Quinn,et al.  The structure and thermotropic properties of pure 1,2-diacylgalactosylglycerols in aqueous systems. , 1981, Biochimica et biophysica acta.

[47]  A. Baracca,et al.  Dynamics of biological membranes. , 1988, Annali dell'Istituto superiore di sanita.