Portable multichannel fiber optic biosensor for field detection

A compact, portable fiber optic biosensor is developed that enables monitoring of up to four fiber optic probes simultaneously. The sensor employs a novel optical fiber bundle jumper for exciting and col- lecting fluorescence emission from the evanescent wave fiber optic probes. A single fiber in the center of the bundle couples laser excitation into the sensor probe, while the surrounding fibers collect the returning fluorescent emission light. This design requires no beamsplitter, enabling the detection optics and control circuitry to be reduced to a 436 in. circuit card. Four of these cards are integrated into a single portable system. Results from detection assays for hazardous biological agents and an environmental pollutant are shown. © 1997 Society of Photo-Optical Instrumen- tation Engineers. (S0091-3286(97)01404-9)

[1]  Frances S. Ligler,et al.  On-site detection of TNT with a portable fiber optic biosensor , 1997 .

[2]  G.P. Anderson,et al.  Development of an evanescent wave fiber optic biosensor , 1994, IEEE Engineering in Medicine and Biology Magazine.

[3]  J M Calvert,et al.  Use of thiol-terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces. , 1989, Analytical biochemistry.

[4]  Frances S. Ligler,et al.  Fluorometer and tapered fiber optic probes for sensing in the evanscent wave , 1992 .

[5]  Frances S. Ligler,et al.  Fiber-Optic Biosensor for the Detection of Hazardous Materials , 1993 .

[6]  Dietrich Marcuse,et al.  Launching light into fiber cores from sources located in the cladding , 1988 .

[7]  Frances S. Ligler,et al.  A fiber optic biosensor: combination tapered fibers designed for improved signal acquisition , 1993 .

[8]  C. J. Lewis,et al.  Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. , 1993, Bioconjugate chemistry.

[9]  F. Ligler,et al.  Quantitating staphylococcal enterotoxin B in diverse media using a portable fiber-optic biosensor. , 1996, Analytical biochemistry.

[10]  G. Stewart.  Optical Waveguide Theory , 1983, Handbook of Laser Technology and Applications.

[11]  David W. Conrad,et al.  Detection of TNT in Water Using an Evanescent Wave Fiber-Optic Biosensor , 1995 .

[12]  Frances S. Ligler,et al.  Use of cyanine dyes with evanescent wave fiber optic biosensors , 1994, Optics & Photonics.

[13]  Elric W. Saaski,et al.  Multimode evanescent wave-based sensors: enhancement strategies , 1995, Pacific Northwest Fiber Optic Sensor.