Tuneable and robust long range surface plasmon resonance for biosensing applications

Abstract A multilayered biosensing architecture based on long range surface plasmons (LRSPs) is reported. LRSPs originate from the coupling of surface plasmons on the opposite sides of a thin metal film embedded in a symmetrical refractive index environment. With respect to regular SPs, LRSPs are characterized by extended electromagnetic field profiles and lower losses, making them of high interest in biosensing, especially for large biological entities. LRSPs-supporting layer structures are typically prepared by using fluoropolymers with refractive indices close to that of water. Unfortunately, fluoropolymers have low surface energies which can translate into poor adhesion to substrates and sub-optimal properties of coatings with surface plasmon resonance-active metal layers such as gold. In this work, a multilayered fluoropolymer structure with tuneable average refractive index is described and used to adjust the penetration depth of LRSP from the sensor surface. The proposed methodology also provides a simple solution to increase the adhesion of LRSP-supporting structures to glass substrates. Towards taking full advantage of long range surface plasmon resonance sensors, a novel approach based on the plasma-polymerization of allylamine is also described to improve the quality of gold layers on fluoropolymers such as Teflon AF. Through these advancements, long range surface plasmon resonance sensors were fabricated with figures of merit as high as 466 RIU −1 . The remarkable performance of these sensors combined with their high stability is expected to foster applications of LRSPR in biosensing.

[1]  Jirí Homola,et al.  Quantitative and simultaneous detection of four foodborne bacterial pathogens with a multi-channel SPR sensor. , 2006, Biosensors & bioelectronics.

[2]  Joseph Maria Kumar Irudayaraj,et al.  Rapid detection of Salmonella enteritidis and Escherichia coli using surface plasmon resonance biosensor , 2006 .

[3]  Michihiro Hide,et al.  Real-time analysis of ligand-induced cell surface and intracellular reactions of living mast cells using a surface plasmon resonance-based biosensor. , 2002, Analytical biochemistry.

[4]  Ismail Hakki Boyaci,et al.  Rapid and label‐free bacteria detection by surface plasmon resonance (SPR) biosensors , 2009, Biotechnology journal.

[5]  D. Sarid.  Long-Range Surface-Plasma Waves on Very Thin Metal Films , 1981 .

[6]  Radan Slavik,et al.  Advanced biosensing using simultaneous excitation of short and long range surface plasmons , 2006 .

[7]  Milan Vala,et al.  Long-range surface plasmons for sensitive detection of bacterial analytes , 2009 .

[8]  Hidenori Suzuki,et al.  Surface plasmon resonance biosensor detects the downstream events of active PKCbeta in antigen-stimulated mast cells. , 2008, Biosensors & bioelectronics.

[9]  Yi Wang,et al.  Bacterial pathogen surface plasmon resonance biosensor advanced by long range surface plasmons and magnetic nanoparticle assays. , 2012, Analytical chemistry.

[10]  K. S. Siow,et al.  Plasma Methods for the Generation of Chemically Reactive Surfaces for Biomolecule Immobilization and Cell Colonization ‐ A Review , 2006 .

[11]  J. Homola,et al.  Spectroscopy of Bragg-scattered surface plasmons for characterization of thin biomolecular films. , 2007, Optics letters.

[12]  Yi Wang,et al.  Prostate specific antigen biosensor based on long range surface plasmon-enhanced fluorescence spectroscopy and dextran hydrogel binding matrix. , 2009, Analytical chemistry.

[13]  N. Halas,et al.  Tailoring plasmonic substrates for surface enhanced spectroscopies. , 2008, Chemical Society reviews.

[14]  W. Knoll,et al.  Optimization of layer structure supporting long range surface plasmons for surface plasmon-enhanced fluorescence spectroscopy biosensors , 2010 .

[15]  W. Knoll,et al.  Long range surface plasmon and hydrogel optical waveguide field-enhanced fluorescence biosensor with 3D hydrogel binding matrix: on the role of diffusion mass transfer. , 2010, Biosensors & bioelectronics.

[16]  Benjamin Thierry,et al.  Reactive epoxy-functionalized thin films by a pulsed plasma polymerization process. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[17]  J. R. Sambles,et al.  Optical characterisation of gold using surface plasmon-polaritons , 1987 .

[18]  J. Homola.  Surface plasmon resonance sensors for detection of chemical and biological species. , 2008, Chemical reviews.

[19]  Radan Slavik,et al.  Ultrahigh resolution long range surface plasmon-based sensor , 2007 .

[20]  H. Raether.  Surface Plasmons on Smooth and Rough Surfaces and on Gratings , 1988 .

[21]  W. Knoll,et al.  Long Range Surface Plasmons for Observation of Biomolecular Binding Events at Metallic Surfaces , 2007 .

[22]  Wolfgang Knoll,et al.  Biosensors based on surface plasmon-enhanced fluorescence spectroscopy (Review) , 2008, Biointerphases.

[23]  R. Corn,et al.  Long-range surface plasmon resonance imaging for bioaffinity sensors. , 2005, Analytical chemistry.

[24]  C Bechinger,et al.  Imaging of cell/substrate contacts of living cells with surface plasmon resonance microscopy. , 1999, Biophysical journal.

[25]  R. Frontiera,et al.  SERS: Materials, applications, and the future , 2012 .

[26]  Jiří Homola,et al.  Long-range surface plasmons for high-resolution surface plasmon resonance sensors , 2001 .

[27]  Van Os.  Surface modification by plasma polymerization: film deposition, tailoring of surface properties and biocompatibility , 2000 .

[28]  Shaoyi Jiang,et al.  Interactions of alginate-producing and -deficient Pseudomonas aeruginosa with zwitterionic polymers. , 2012, Biomaterials.

[29]  P. Berini.  Long-range surface plasmon polaritons , 2009 .