Production of a penicillin-like antibiotic by Trichophyton mentagrophytes on an agar-based medium containing skin keratin as the major nutrient.

A culture medium composed of powdered human plantar callus, synthetic sweat and agar, was used to study the synthesis of a penicillin-like antibiotic by a strain of Trichophyton mentagrophytes grown in vitro. Series of 5 mm agar plug samples, taken radially across single colonies of the dermatophyte, were bioassayed for antibiotic, so that a profile of antibiotic concentration could be prepared. It was found that in colonies of 11 d and older, grown at 33°, antibiotic concentration was highest in the area of the colony edge.

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